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Chapter 12.5 : Matrix-Assisted Laser Desorption Ionization Time of Flight (MALDI-TOF) for Microorganism Identification

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Matrix-Assisted Laser Desorption Ionization Time of Flight (MALDI-TOF) for Microorganism Identification, Page 1 of 2

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Abstract:

Identification of bacteria and fungi in the clinical microbiology laboratory has traditionally relied on a combination of phenotypic characteristics, including Gram stain results, colony morphology, growth characteristics, and biochemical reactions. More recently, sequencing of marker genes with both conserved and variable regions, such as 16S and 18S rRNA, has enhanced the laboratory’s capacity to accurately identify microorganisms. Many of these identification methodologies are both time- and labor-intensive, while others, such as sequencing, are quite costly. Therefore, more rapid and cost-effective methods for routine identification of microorganisms are desired.

Citation: Leber A. 2016. Matrix-Assisted Laser Desorption Ionization Time of Flight (MALDI-TOF) for Microorganism Identification, p 12.5.1-12.5.13. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch12.5
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References

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1. Singhal N, Kumar M, Kanaujia PK, Virdi JS. 2015. MALDI-TOF mass spectrometry: an emerging technology for microbial identification and diagnosis. Front Microbiol 6:791. doi: 10.3389/fmicb.2015.00791.
2. Patel R. 2015. MALDI-TOF MS for the diagnosis of infectious diseases. Clin Chem 61:100111.
3. bioMérieux, Inc. 2013. VITEK MS Workflow User Manual Clinical US Version. bioMérieux, Inc., Marcy-l’Étoile, France
4. bioMérieux, Inc. K124067 Decision Summary. bioMérieux, Inc., Marcy-l’Étoile, France
5. Bruker Daltonics Inc. K142677 Substantial Equivalence Determination Decision Memorandum. Bruker Daltonics Inc., Billerica, MA.
6. Bruker Daltonics Inc. K130831 Substantial Equivalence Determination Decision Summary. Bruker Daltonics Inc., Billerica, MA.
7. Valentine N, Wunschel S, Wunschel D, Petersen C, Wahl K. 2005. Effect of culture conditions on microorganism identification by matrix-assisted laser desorption ionization mass spectrometry. Appl Environ Microbiol 71:5864.
8. Ilina EN. 2009. Direct matrix-assisted laser desorption-ionisation (MALDI) mass-spectrometry bacteria profiling for identifying and characterizing pathogens. Acta Naturae 1:115120.
9. Verroken A, Janssens M, Berhin C, Bogaerts P, Huang TD, Wauters G, Glupczynski Y. 2010. Evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of nocardia species. J Clin Microbiol 48:40154021.
10. El Khechine A, Couderc C, Flaudrops C, Raoult D, Drancourt M. 2011. Matrix-assisted laser desorption/ionization time-offlight mass spectrometry identification of mycobacteria in routine clinical practice. PLoS One 6:e24720. doi: 10.1371/journal.pone.0024720.
11. Stevenson LG, Drake SK, Shea YR, Zelazny AM, Murray PR. 2010. Evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of clinically important yeast species. J Clin Microbiol 48:34823486.
12. Theel ES, Schmitt BH, Hall L, Cunningham SA, Walchak RC, Patel R, Wengenack NL. 2012. Formic acid-based direct, on-plate testing of yeast and Corynebacterium species by Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol 50:30933095.
13. Lau AF, Drake SK, Calhoun LB, Henderson CM, Zelazny AM. 2013. Development of a clinically comprehensive database and a simple procedure for identification of molds from solid media by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol 51:828834.
14. Bizzini A, Durussel C, Bille J, Greub G, Prod’hom G. 2010. Performance of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of bacterial strains routinely isolated in a clinical microbiology laboratory. J Clin Microbiol 48:15491554.
15. Pavlovic M, Konrad R, Iwobi AN, Sing A, Busch U, Huber I. 2012. A dual approach employing MALDI-TOF MS and real-time PCR for fast species identification within the Enterobacter cloacae complex. FEMS Microbiol Lett 328:4653.
16. Fehlberg LC, Andrade LH, Assis DM, Pereira RH, Gales AC, Marques EA. 2013. Performance of MALDI-ToF MS for species identification of Burkholderia cepacia complex clinical isolates. Diagn Microbiol Infect Dis 77:126128.
17. Seng P, Drancourt M, Gouriet F, La Scola B, Fournier PE, Rolain JM, Raoult D. 2009. Ongoing revolution in bacteriology: routine identification of bacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Clin Infect Dis 49:543551.
18. van Veen SQ, Claas EC, Kuijper EJ. 2010. High-throughput identification of bacteria and yeast by matrix-assisted laser desorption ionization-time of flight mass spectrometry in conventional medical microbiology laboratories. J Clin Microbiol 48:900907.
19. Association of Public Health Laboratories. 2015. Evaluation of Biothreat Agent Growth with Three Matrix Assisted Laser Desorption/Ionization Time of Flight (MALDI TOF) Mass Spectrometry Preparation Methods. Association of Public Health Laboratories, Silver Spring, MD.

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