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Chapter 12.5 : Matrix-Assisted Laser Desorption Ionization Time of Flight (MALDI-TOF) for Microorganism Identification
Matrix-Assisted Laser Desorption Ionization Time of Flight (MALDI-TOF) for Microorganism Identification, Page 1 of 2< Previous page Next page > /docserver/preview/fulltext/10.1128/9781555818814/9781555818814_Chap12.5-1.gif /docserver/preview/fulltext/10.1128/9781555818814/9781555818814_Chap12.5-2.gif
Identification of bacteria and fungi in the clinical microbiology laboratory has traditionally relied on a combination of phenotypic characteristics, including Gram stain results, colony morphology, growth characteristics, and biochemical reactions. More recently, sequencing of marker genes with both conserved and variable regions, such as 16S and 18S rRNA, has enhanced the laboratory’s capacity to accurately identify microorganisms. Many of these identification methodologies are both time- and labor-intensive, while others, such as sequencing, are quite costly. Therefore, more rapid and cost-effective methods for routine identification of microorganisms are desired.