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Chapter 3.12 : Urine Cultures
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Urinary tract infections (UTIs) are extremely common. They account for 8 million visits to physicians’ offices and over 100,000 hospital admissions per year ( 1 ), with an estimated cost of $1.6 billion in the United States alone ( 2 ).
(a) Method of streaking urine for colony count using a 1-μl loop; (b) method of streaking urine for colony count using either a 10- or 1-μl loop; (c) spreader method of streaking urine for colony count after inoculation with either a 10- or 1-μl volume; (d) method of streaking 10 μl of urine using drip method for count and quadrant streaking for isolation; (e) MAC-CNA biplate with 10 μl of urine streaked in quadrants (EMB and PEA are other options).
Flowchart for forward pipetting (adapted from reference 8 with permission).
Flowchart for reverse pipetting (adapted from reference 8 with permission).
Colorimetric Quantitative Loop Calibration
Urinary microbiota a
Summary of chromogenic agar and pigmentation of colonies a
Summary of bacteriological culture systems a
Protocol for workup of urine cultures
Reporting of isolates with minimal testing a
Calibration methods for volume-dispensing instruments
Commercial pipettor calibration systems and related products
Suggested calibration schedule for microbiology laboratory pipettors
Z value for distilled water as a function of temperature and pressure a
Theoretical absorbance values for test solutions
Suggested tolerance limits for microbiology pipettors