1887

Chapter 4.10 : Use of Matrix-Assisted Laser Desorption Ionization–Time of Flight for the Identification of Anaerobic Bacteria

MyBook is a cheap paperback edition of the original book and will be sold at uniform, low price.

Ebook: Choose a downloadable PDF or ePub file. Chapter is a downloadable PDF file. File must be downloaded within 48 hours of purchase

Buy this Chapter
Digital (?) $30.00

Preview this chapter:
Zoom in
Zoomout

Use of Matrix-Assisted Laser Desorption Ionization–Time of Flight for the Identification of Anaerobic Bacteria, Page 1 of 2

| /docserver/preview/fulltext/10.1128/9781555818814/9781555818814_Chap4.10-1.gif /docserver/preview/fulltext/10.1128/9781555818814/9781555818814_Chap4.10-2.gif

Abstract:

The use of matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) for the identification of aerobic bacteria has begun to take hold in many clinical laboratories. A large amount of data has been published about how well it has performed for most aerobic organisms. There were a few articles early on about its use for the identification of anaerobic bacteria that suggested it needed improvements before it could be implemented in clinical laboratories; however, more recently, there have been at least 12 publications that demonstrate the potential for using MALDI-TOF for the successful identification of anaerobes as well, as has already been demonstrated for aerobes and other microbes. In this procedure, there will be a brief introduction into the principles of MALDI as they relate to the identification of bacteria, mention of the systems that have been or should be cleared by the US Food and Drug Administration (FDA) for use in clinical laboratories in the future, a summary of some of the work that has been done in the area of anaerobic bacterial identification, and a potential scheme for how it might be used routinely in the clinical laboratory for the identification of anaerobes. This procedure will not, however, describe the actual procedures for performing a MALDI-TOF in the clinical laboratory. Both of the products’ manufacturers and distributors, which have been cleared or are awaiting FDA clearance in the United States, have great technical teams and ample literature that can provide those procedures and suggestions for the use of MALDI in the clinical laboratory.

Citation: Hall G. 2016. Use of Matrix-Assisted Laser Desorption Ionization–Time of Flight for the Identification of Anaerobic Bacteria, p 4.10.1-4.10.5. In Leber A (ed), Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch4.10
Highlighted Text: Show | Hide
Loading full text...

Full text loading...

References

/content/book/10.1128/9781555818814.chap4.10
1. Anhalt JP, Fenselau JP. 1975. Identification of bacteria using mass spectrometry. Anal Chem 47:219225.
2. Biswas S, Rolain JM. 2013. Use of MALDITOF mass spectrometry for identification of bacteria that are difficult to culture. J Microbiol Methods 92:1424.
3. Croxatto A, Prod’hom G, Greub G. 2012. Applications of MALDI-TOF mass spectrometry in clinical diagnostic microbiology. FEMS Microbiol Rev 36:380407.
4. Bizzini A, Greub G. 2010. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry, a revolution in clinical microbial identification. Clin Microbiol Infect 16:16141619.
5. Cherkaoui A, Hibbs J, Emonet S, Tangomo M, Girard M, Francois P, Schrenzel J. 2010. Comparison of two matrix-assisted laser desorption ionization-time of flight mass spectrometry methods with conventional phenotypic identification for routine identification of bacteria to the species level. J Clin Microbiol 48:11691675.
6. Culebras E, Rodriguez-Avial I, Betriu C, Gomez M, Picazo JJ. 2012. Rapid identification of clinical isolates of Bacteroides species by matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry. Anaerobe 18:163165.
7. Jurtesen US, Holm A, Knudsen E, Andersen LB, Jensen TG, Kemp M, Skov MN, Hansen BG, Moller JK. 2011. Species identification of clinical isolates of anaerobic bacteria: a comparison of two matrix-assisted laser desorption ionization-time of flight mass spectrometry systems. J Clin Microbiol 49:43154318.
8. Veloo ACM, Knoester M, Degener JE, Kuijper EJ. 2011. Comparison of two matrix-assisted laser desorption ionization-time of flight mass spectrometry methods for the identification of clinically relevant anaerobic bacteria. Clin Microbiol Infect 17:15011706.
9. Fedorko DP, Drake SK, Murray PR. 2012. Identification of clinical isolates of anaerobic bacteria using matrix-assisted laser desorption ionization-time of flight mass spectrometry. Eur J Clin Microbiol 31:22572262.
10. Veloo ACM, Erhard M, Welker M, Welling GW, Degener JE. 2011. Identification of gram positive anaerobic cocci by MALDITOF mass spectrometry. Syst Appl Microbiol 34:5862.
11. Meex C, Neuville F, Descy J, Huynen P, Hayette MP, DeMol P, Melin P. 2012. Direct identification of bacteria from BacT/Alert anaerobic positive blood cultures by MALDITOF MS: MALDI Sepsityper kit versus an in-house saponin method for bacterial extraction. J Med Microbiol 61:15111516.
12. LaScola B, Fournier PE, Raoult D. 2011. Burden of emerging anaerobes in the MALDI-TOF and 16S rRNA gene sequencing era. Anaerobe 17:106112.
13. Fournier R, Wallet F, Grandbastien B, Dubreuil L, Courcol R, Neut C, Dessein R. 2012. Chemical extraction versus direct smear for MALDI-TOF mass spectrometry identification of anaerobe bacteria. Anaerobe 18:294297.
14. Nagy E, Becker S, Kostrzewa M, Barta N, Urban E. 2012. The value of MALDI-TOF MS for the identification of clinically relevant anaerobic bacteria in routine laboratories. J Med Microbiol 61:13931400.
15. Schmitt BH, Cunningham SA, Dailey AL, Gustafson DR, Patel R. 2013. Identification of anaerobic bacteria by Bruker Biotyper Matrix-assisted laser desorption ionization-time of flight mass spectrometry with on-plate formic acid preparation. J Clin Microbiol 51:782786.
16. Wybo I, Soetens O, DeBel A, Echahidi F, Vancutsem E, Vandoorslaer K, Pierard D. 2012. Species identification of clinical Prevotella isolates by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol 50:14151418.
17. Dols JA, Smit PW, Kort R, Reid G, Schuren FH, Tempelman H, Bontekoe TR, Korporaal H, Boon ME. 2011. Microarray-based identification of clinically relevant vaginal bacteria in relation to bacterial vaginosis. Am J Obstet Gyn 204:305.e17.
18. Lin YT, Vaneechoutte M, Huang AH, Teng LJ, Chen HM, Su SL, Chang TC. 2010. Identification of clinically important anaerobic bacteria by an oligonucleotide array. J Clin Microbiol 48:12831290.
19. Papaparaskevas J, Mela V, Houhoula DP, Pantazatou A, Petrikkos GL, Tsakris A. 2013. Comparative evaluation of conventional and real-time PCR assays for detecting Bacteroides fragilis in clinical samples. J Clin Microbiol 51:15931595.
20. Tong J, Liu C, Summanen P, Xu H, Finegold SM. 2011. Application of quantitative real-time PCR for rapid identification of Bacteroides fragilis group and related organisms in human wound samples. Anaerobe 17:6468.
21. Woo PCY, Fung AM, Lau SK, Hon E, Yuen KY. 2002. Diagnosis of pelvic actinomycosis by 16S rRNA gene sequencing and its clinical significance. Diagn Mcrobiol Infect Dis 43:113118.
22. Woo PCY, Teng JLL, Yeung JMY, Tse H, Lau SKP, Yuen KY. 2011. Automated identification of medically important bacteria by 16S rRNA gene sequencing using a novel comprehensive database, 16SpathDB. J Clin Microbiol 49:17991809.
23. Yeoman CJ, Thomas SM, Miller ME, Ulanov AV, Torralba M, Lucas S, Gillis M, Cregger M, Gomez A, Ho M, Leigh SR, Stumpf R, Creedon DJ, Smith MA, Weisbaum JS, Nelson KE, Wilson BA, White BA. 2013. A multi-omic systems-based approach reveals metabolic markers of bacterial vaginosis and insight into the disease. PLOS One 8:e56111.

Tables

Generic image for table
Table 4.10–1

Publications on use of MALDI-TOF for the identification of anaerobes

Citation: Hall G. 2016. Use of Matrix-Assisted Laser Desorption Ionization–Time of Flight for the Identification of Anaerobic Bacteria, p 4.10.1-4.10.5. In Leber A (ed), Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch4.10

This is a required field
Please enter a valid email address
Please check the format of the address you have entered.
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error