1887

Chapter 4.2 : Collection, Transport, and Processing of Clinical Specimens for Anaerobic Culture

MyBook is a cheap paperback edition of the original book and will be sold at uniform, low price.

Ebook: Choose a downloadable PDF or ePub file. Chapter is a downloadable PDF file. File must be downloaded within 48 hours of purchase

Buy this Chapter
Digital (?) $30.00

Preview this chapter:
Zoom in
Zoomout

Collection, Transport, and Processing of Clinical Specimens for Anaerobic Culture, Page 1 of 2

| /docserver/preview/fulltext/10.1128/9781555818814/9781555818814_Chap4.2-1.gif /docserver/preview/fulltext/10.1128/9781555818814/9781555818814_Chap4.2-2.gif

Abstract:

Proper collection of specimens to avoid contamination with organisms of the normal microbiota and prompt transport to the laboratory for processing are essential. The isolation of anaerobic bacteria from appropriately collected clinical samples and reporting of that information as quickly as possible are extremely important for the clinician to be able to implement appropriate therapeutics. Gram stains are needed to provide a rapid analysis of the appropriateness of the sample and rapid information about potential pathogens, and they are essential for the correlation to what grows out in the laboratory.

Citation: Hall G, Holden J. 2016. Collection, Transport, and Processing of Clinical Specimens for Anaerobic Culture, p 4.2.1-4.2.8. In Leber A (ed), Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch4.2
Highlighted Text: Show | Hide
Loading full text...

Full text loading...

References

/content/book/10.1128/9781555818814.chap4.2
1. Baron E, Thomson RB. 2011. Specimen collection, transport, and processing: bacteriology, p 228271. In Versalovic J, Carroll KC, Funke G, Jorgensen JH, Landry ML, Warnock DW (ed), Manual of Clinical Microbiology, 10th ed. ASM Press, Washington, DC.
2. Jousimies-Somer HR, Summanen P, Citron DM, Baron EJ, Wexler HM, Finegold SM. 2002. Wadsworth Anaerobic Bacteriology Manual, 6th ed. Star Publishing Co., Belmont, CA.
3. Miller JM. 1999. A Guide to Specimen Management in Clinical Microbiology, 2nd ed. ASM Press, Washington, DC.
4. Citron DM, Warren YA, Hudspeth MK, Goldstein EJC. 2000. Survival of aerobic and anaerobic bacteria in purulent clinical specimens maintained in the Copan Venturi Transystem and Becton Dickinson Port-A-Cul transport systems. J Clin Microbiol 38:892894.
5. Hindiyeh M, Acevedo V, Carroll KC. 2001. Comparison of three transport systems (Starplex StarSwab II, the new Copan ViPak Amies agar gel collection and transport swabs, and BBL Port-A-Cul) for maintenance of anaerobic and fastidious aerobic organisms. J Clin Microbiol 39:377380.
6. Van Horn KG, Audette CD, Tucker KA, Sebeck D. 2008. Comparison of 3 swab transport systems for direct release and recovery of aerobic and anaerobic bacteria. Diagn Microbiol Infect Dis 62:471473.
7. Stoner KA, Rabe LK, Austin MN, Meyn LA, Hillier SL. 2008. Quantitative survival of aerobic and anaerobic microorganisms in Port-A-Cul and Copan Transport Systems. J Clin Microbiol 46:27392744.
8. Van Horn KG, Audette CD, Sebeck D, Tucker KA. 2008. Comparison of the Copan ESwab System with two Amies agar swab transport systems for maintenance of microorganism viability. J Clin Microbiol 46:16551458.
9. Pellizzer G, Strazzabosco M, Presi S, Furlan F, Lora L, Benedetti P, Bonato M, Erle G, de Lalla F. 2001. Deep tissue biopsy vs. superficial swab culture monitoring in the microbiological assessment of limb-threatening diabetic foot infection. Diabet Med 18:822827.
10. Dowd SE, Sun Y, Secor PR, Rhoads DD, Wolcott BM, James GA, Wolcott RD. 2008. Survey of bacterial diversity in chronic wounds using pyrosequencing, DGGE, and full ribosome shotgun sequencing. BMC Microbiol 8:43.
11. Dowd SE, Wolcott RD, Sun Y, McKeehan T, Smith E, Rhoads D. 2008. Polymicrobial nature of chronic diabetic foot ulcer biofilm infections determined using bacterial tag encoded FLX amplicon pyrosequencing (bTEFAP). PLoS ONE 10:e3326.
12. Kessler L, Piemont Y, Ortega F, Lesens O, Boeri C, Averous C, Meyer R, Hansmann Y, Christmann D, Gaudias J, Pinget M. 2006. Comparison of microbiological results of needle puncture vs. superficial swab in infected diabetic foot ulcer with osteomyelitis. Diabet Med 23:99102.
13. Senneville E, Melliez H, Beltrand E, Legout L, Valette M, Cazaubiel M, Cordonnier M, Caillaux M, Yazdanpanah Y, Mouton Y. 2005. Culture of percutaneous bone biopsy specimens for diagnosis of diabetic foot osteomyelitis: concordance with ulcer swab cultures. Clin Infect Dis 42:5762.
14. Baron EJ, Vaïsänen ML, McTeague M, Strong CA, Norman D, Finegold SM. 1993. Comparison of the Accu-CulShure system and a swab placed in a B-D Port-a-Cul tube for specimen collection and transport. Clin Infect Dis 16(Suppl 4):S325S327.
15. Westhoff C. 2007. Intrauterine devices and colonization or infection with Actinomyces. Contraception 75:S48S50.
16. Quercia R, Bani Sadr F, Cortez A, Arlet G, Pialoux G. 2006. Genital tract actinomycosis caused by Actinomyces israelii. Med Mal Infect 36:393395.
17. Wu SM, Burns EM, Pottinger PS, Margaret AS, Rakeman JL, Matsen FA, Cookson BT. 2001. Optimization of periprosthetic culture for diagnosis of Propionibacterium acnes prosthetic joint infection. J Clin Microbiol 49:24902495.
18. Trampuz A, Piper KE, Jacobson MJ, Hanssen AD, Unni KK, Osmon DR, Mandrekar JN, Cockerill FR, Steckelberg JM, Greenleaf JF, Patel R. 2007. Sonication of removed hip and knee prostheses for diagnosis of infection. N Engl J Med 357:654663.
19. Valle CD, Parvizi J, Bauer TW, DiCesare PE, Evand RP, Segreti J, Spangehl M, Watters WC, Keith M, Turkelson CM, Wies JI, Sluka P, Hitchcock K. 2010. Diagnosis of periprosthetic joint infections of the hip and knee. J Am Acad Orthop Surg 18:760770.
20. Finegold SM, George WL. 1989. Anaerobic Infections in Humans. Academic Press, Inc., San Diego, CA.
21. Forbes BA, Sahm DF, Weissfeld AS (ed). 2007. Bailey and Scott’s Diagnostic Bacteriology, 12th ed. Mosby Elsevier, St. Louis, MO.
22. Mangels JI. 1994. Anaerobic transport systems: are they necessary? Clin Microbiol Newsl 16:101104.

Tables

Generic image for table
Table 4.2–1

Acceptable specimens for anaerobic culture

Citation: Hall G, Holden J. 2016. Collection, Transport, and Processing of Clinical Specimens for Anaerobic Culture, p 4.2.1-4.2.8. In Leber A (ed), Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch4.2
Generic image for table
Table 4.2–2

Anaerobic specimen trarisport devices

Citation: Hall G, Holden J. 2016. Collection, Transport, and Processing of Clinical Specimens for Anaerobic Culture, p 4.2.1-4.2.8. In Leber A (ed), Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch4.2
Generic image for table
Table 4.2–3

Suggested transport times for certain specimen volumes and collection methods

Citation: Hall G, Holden J. 2016. Collection, Transport, and Processing of Clinical Specimens for Anaerobic Culture, p 4.2.1-4.2.8. In Leber A (ed), Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch4.2
Generic image for table
Untitled

Citation: Hall G, Holden J. 2016. Collection, Transport, and Processing of Clinical Specimens for Anaerobic Culture, p 4.2.1-4.2.8. In Leber A (ed), Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch4.2
Generic image for table
Untitled

Citation: Hall G, Holden J. 2016. Collection, Transport, and Processing of Clinical Specimens for Anaerobic Culture, p 4.2.1-4.2.8. In Leber A (ed), Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch4.2
Generic image for table
Untitled

Citation: Hall G, Holden J. 2016. Collection, Transport, and Processing of Clinical Specimens for Anaerobic Culture, p 4.2.1-4.2.8. In Leber A (ed), Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch4.2
Generic image for table
Untitled

Citation: Hall G, Holden J. 2016. Collection, Transport, and Processing of Clinical Specimens for Anaerobic Culture, p 4.2.1-4.2.8. In Leber A (ed), Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch4.2
Generic image for table
Untitled

Citation: Hall G, Holden J. 2016. Collection, Transport, and Processing of Clinical Specimens for Anaerobic Culture, p 4.2.1-4.2.8. In Leber A (ed), Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch4.2

This is a required field
Please enter a valid email address
Please check the format of the address you have entered.
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error