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Chapter 4.3 : Culture Media for Anaerobes

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Culture Media for Anaerobes, Page 1 of 2

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Abstract:

The choice of media for use in the anaerobic bacteriology laboratory is important for the success of anaerobic bacteriology. The media must contain appropriate nutrients and supplements needed by clinically significant anaerobes. A combination of enriched, nonselective, selective, and differential media should be used for the initial processing, isolation, and presumptive identification of anaerobic bacteria from clinical specimens ( Fig. 4.3–1 and Tables 4.3–1 and 4.3–2 ) ( ). Anaerobes have a wide range of nutritional needs; most, however, require hemin and vitamin K. Some studies suggest that freshly prepared, properly stored, highly enriched media are essential for recovery of anaerobes (5, ), while another study has shown that pre-reduced anaerobically sterilized (PRAS) media best support the growth of anaerobes ( ). Recent studies have suggested that using media containing oxyrase (Oxyrase, Inc., Ohio) may be another alternative ( ). Media that have been exposed to air contain oxidized products that may delay or inhibit the growth of many anaerobes. The ideal media for use in anaerobic bacteriology, therefore, are those that have had limited exposure to oxygen.

Citation: Hall G, Mangels J. 2016. Culture Media for Anaerobes, p 4.3.1-4.3.10. In Leber A (ed), Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch4.3
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Figures

Image of Figure 4.3–1
Figure 4.3–1

Flowchart for processing primary anaerobic culture plates. anaBAP, anaerobic blood agar plate; CAP, chocolate agar plates; RBA, rabbit blood agar.

Citation: Hall G, Mangels J. 2016. Culture Media for Anaerobes, p 4.3.1-4.3.10. In Leber A (ed), Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch4.3
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References

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1. Atlas RM, Snyder JW. 2011. Reagents, stains, and media: bacteriology, p 272303. In Versalovic J, Carroll KC, Funke G, Jorgensen JH, Landry ML, Warnock DW (ed), Manual of Clinical Microbiology, 10th ed. ASM Press, Washington, DC.
2. Dowell VRJr, Lombard GL, Thompson FS, Armfield AY. 1977. Media for Isolation, Characterization, and Identification of Obligately Anaerobic Bacteria. CDC laboratory manual. Centers for Disease Control and Prevention, Atlanta, GA.
3. Forbes BA, Sahm DF, Weissfeld AS (ed). 2007. Bailey and Scott’s Diagnostic Microbiology, 12th ed. Mosby Elsevier, St. Louis, MO.
4. Jousimies-Somer HR, Summanen P, Citron DM, Baron EJ, Wexler HM, Finegold SM. 2002. Wadsworth Anaerobic Bacteriology Manual, 6th ed. Star Publishing Co., Belmont, CA.
5. Hanson CW, Martin WJ. 1976. Evaluation of enrichment, storage, and age of blood agar medium in relation to its ability to support growth of anaerobic bacteria. J Clin Microbiol 4:394399.
6. Murray PR. 1978. Growth of clinical isolates of anaerobic bacteria on agar media: effects of media composition, storage conditions, and reduction under anaerobic conditions. J Clin Microbiol 8:708714.
7. Mangels JI, Douglas BP. 1989. Comparison of four commercial brucella agar media for growth of anaerobic organisms. J Clin Microbiol 27:22682271.
8. Thurston M, Maida D, Gannon C. 2000. Oxyrase cell-membrane preparations simplify cultivation of anaerobic bacteria. Lab Med 31:509512.
9. Wiggs L, Cavallaro J, Miller M. 1998. Evaluation of oxyrase OxyPlate anaerobe incubation system, abstr. C-449. Abstr 98th Gen Meet Am Soc Microbiol American Society for Microbiology, Washington, DC.
10. Finegold SM, Sugihara PT, Sutter VL. 1971. Use of selective media for isolation of anaerobes, p 99108. In Shapton DA, Board RG (ed), Isolation of Anaerobes. Academic Press, Inc., London, England.
11. Livingston SJ, Kominos SD, Yee RB. 1978. New medium for selection and presumptive identification of the Bacteroides fragilis group. J Clin Microbiol 7:448453.
12. Deschler EK, Thompson PP, Kowalski RP. 2012. Evaluation of the new OxyPlate ™ Anaerobic system for the isolation of ocular anaerobic bacteria. Int J Ophthalmol 5:582585.

Tables

Generic image for table
Table 4.3–1

Anaerobic media and their uses

Citation: Hall G, Mangels J. 2016. Culture Media for Anaerobes, p 4.3.1-4.3.10. In Leber A (ed), Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch4.3
Generic image for table
Table 4.3–2

Additional media for use in cultivating or selecting for specific anaerobes

Citation: Hall G, Mangels J. 2016. Culture Media for Anaerobes, p 4.3.1-4.3.10. In Leber A (ed), Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch4.3
Generic image for table
Table 4.3–3

Recommended primary medium setup

Citation: Hall G, Mangels J. 2016. Culture Media for Anaerobes, p 4.3.1-4.3.10. In Leber A (ed), Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch4.3

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