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Chapter 4.4 : Incubation Techniques for Anaerobic Bacteriology Specimens

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Incubation Techniques for Anaerobic Bacteriology Specimens, Page 1 of 2

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Abstract:

This procedure describes the various environmental methods and incubation conditions used for anaerobic bacteriology specimens once a properly selected, collected, and transported specimen arrives in the laboratory. Ideally, the specimen is processed immediately on arrival in the laboratory and incubated under anaerobic conditions to prevent further exposure to oxygen. Refer to procedure 4.2 for collection and processing procedures and to procedure 4.3 for anaerobic media and for inoculation techniques.

Citation: Hall G, Mangels J. 2016. Incubation Techniques for Anaerobic Bacteriology Specimens, p 4.4.1-4.4.4. In Leber A (ed), Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch4.4
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References

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1. Brazier JS, Smith SA. 1989. Evaluation of the Anoxomat: a new technique for anaerobic and microaerophilic clinical bacteriology. J Clin Pathol 42:640645.
2. Shahin M, Jamal W, Verghese T, Rotimi VO. 2003. Comparative evaluation of anoxomat and conventional anaerobic GasPak jar systems for the isolation of anaerobic bacteria. Med Princ Pract 12:8186.
3. Summanen PH, McTeague M, Väisänen ML, Strong CA, Finegold SM. 1999. Comparison of recovery of anaerobic bacteria using the Anoxomat, anaerobic chamber, and GasPak jar systems. Anaerobe 5:59
4. Baron EJ. 2011. Approaches to identification of anaerobic bacteria, p 799802. In Versalovic J, Carroll KC, Funke G, Jorgensen JH, Landry ML, Warnock DW (ed), Manual of Clinical Microbiology, 10th ed. ASM Press, Washington, DC.
5. Engelkirk PG, Duben-Engelkirk J, Dowell VRJr. 1992. Principles and Practice of Clinical Anaerobic Bacteriology. Star Publishing Co., Belmont, CA.
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8. Jousimies-Somer HR, Summanen P, Citron DM, Baron EJ, Wexler HM, Finegold SM. 2002. Wadsworth Anaerobic Bacteriology Manual, 6th ed. Star Publishing Co., Belmont, CA.

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