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Chapter 4.5 : Examination of Primary Culture Plates for Anaerobic Bacteria

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Examination of Primary Culture Plates for Anaerobic Bacteria, Page 1 of 2

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Abstract:

The goal of processing primary culture plates is to isolate significant anaerobic organisms from the original specimen for identification and to perform susceptibility testing when it is indicated. Comparison of culture results with what was seen on the original Gram stain of the specimen is critical. When evaluating culture plates, all morphotypes observed in the original Gram stain should match with the aerobes and anaerobes recovered.

Citation: Hall G, Byrd L. 2016. Examination of Primary Culture Plates for Anaerobic Bacteria, p 4.5.1-4.5.6. In Leber A (ed), Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch4.5
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Figures

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Figure 4.5–1

Procedure for the examination of primary culture plates for anaerobes. Symbols: *, use any other suitable enriched primary media that contain vitamin K and hemin and that allow good growth of anaerobes; †, use EYA if clostridia are suspected from Gram stain or from nature of clinical specimen. KVLB, kanamycin-vancomycinlaked blood agar.

Citation: Hall G, Byrd L. 2016. Examination of Primary Culture Plates for Anaerobic Bacteria, p 4.5.1-4.5.6. In Leber A (ed), Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch4.5
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References

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1. Dowell VR. 1989. Procedures for Isolation and Characterization of Anaerobic Bacteria. Centers for Disease Control and Prevention, Atlanta, GA.
2. Engelkirk PG, Duben-Engelkirk J, Dowell VR. 1992. Principles and Practice of Clinical Anaerobic Bacteriology. Star Publishing Co., Belmont, CA.
3. Forbes BA, Sahm DF, Weissfeld AS (ed). 2007. Bailey and Scott’s Diagnostic Bacteriology, 12th ed. Mosby Elsevier, St. Louis, MO.
4. Holdeman LV, Cato EP, Moore WEC. 1977. Anaerobe Laboratory Manual, 4th ed, p 24, 122, 149. Virginia Polytechnic Institute and State University, Blacksburg, VA.
5. Jousimies-Somer HR, Summanen P, Citron DM, Baron EJ, Wexler HM, Finegold SM. 2002. Wadsworth-KTL Anaerobic Bacteriology Manual, 6th ed. Star Publishing Co., Belmont, CA.
6. Mangels JL, Cox ME, Lindberg LH. 1984. Methanol fixation—an alternative to heat fixation of smears before staining. Diagn Microbiol Infect Dis 2:129137.
7. Jorgensen JH, Pfaller MA, Carroll KC, Funke G, Landry ML, Richter SS, Warnock DW. 2015. Manual of Clinical Microbiology, 11th ed. ASM Press, Washington, DC.

Tables

Generic image for table
Table 4.5–1

Anaerobic organism clues from primary culture plates, and use of supplemental media

Citation: Hall G, Byrd L. 2016. Examination of Primary Culture Plates for Anaerobic Bacteria, p 4.5.1-4.5.6. In Leber A (ed), Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch4.5
Generic image for table
Table 4.5–2

Special-potency antimicrobial agent disks for the identification of anaerobic bacteria

Citation: Hall G, Byrd L. 2016. Examination of Primary Culture Plates for Anaerobic Bacteria, p 4.5.1-4.5.6. In Leber A (ed), Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch4.5

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