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Chapter 4.6 : Rapid Disk, Spot Tests, and Other Rapid or Primary Methods for the Identification of Anaerobes

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Rapid Disk, Spot Tests, and Other Rapid or Primary Methods for the Identification of Anaerobes, Page 1 of 2

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Abstract:

Rapid disk, spot tests, and other methods described in this procedure provide a cost-effective system for the identification of anaerobes. Many of the tests described cost less than $0.50 each to perform ( Appendix 4.6–1 in procedure 4.6.13 for a summary of tests used for the rapid identification of anaerobes).

Citation: Hall G, Summanen P. 2016. Rapid Disk, Spot Tests, and Other Rapid or Primary Methods for the Identification of Anaerobes, p 4.6.1.1-4.6.13.5. In Leber A (ed), Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch4.6
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Figures

Image of Figure 4.6.3–1
Figure 4.6.3–1

Flowchart for nitrate disk reduction test.

Citation: Hall G, Summanen P. 2016. Rapid Disk, Spot Tests, and Other Rapid or Primary Methods for the Identification of Anaerobes, p 4.6.1.1-4.6.13.5. In Leber A (ed), Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch4.6
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References

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1. Engelkirk PG, Duben-Engelkirk J, Dowell VRJr. 1992. Principles and Practice of Clinical Anaerobic Bacteriology. Star Publishing Co., Belmont, CA.
2. Jousimies-Somer HR, Summanen P, Citron DM, Baron EJ, Wexler HM, Finegold SM. 2002. Wadsworth-KTL Anaerobic Bacteriology Manual, 6th ed. Star Publishing Co., Belmont, CA.
3. Lombard GL, Dowell VRJr. 1983. Comparison of three reagents for detecting indole production by anaerobic bacteria in microtest systems. J Clin Microbiol 18:609613.
4. MacFaddin JF. 2000. Biochemical Tests for Identification of Medical Bacteria, 3rd ed. Lippincott Williams & Wilkins, Philadelphia, PA.
5. Engelkirk PG, Duben-Engelkirk J, Dowell VRJr. 1992. Principles and Practice of Clinical Anaerobic Bacteriology. Star Publishing Co., Belmont, CA.
6. Jousimies-Somer HR, Summanen P, Citron DM, Baron EJ, Wexler HM, Finegold SM. 2002. Wadsworth-KTL Anaerobic Bacteriology Manual, 6th ed. Star Publishing Co., Belmont, CA.
7. MacFaddin JF. 2000. Biochemical Tests for Identification of Medical Bacteria, 3rd ed. Lippincott Williams & Wilkins, Philadelphia, PA.
8. Engelkirk PG, Duben-Engelkirk J, Dowell VRJr. 1992. Principles and Practice of Clinical Anaerobic Bacteriology. Star Publishing Co., Belmont, CA.
9. Jousimies-Somer HR, Summanen P, Citron DM, Baron EJ, Wexler HM, Finegold SM. 2002. Wadsworth-KTL Anaerobic Bacteriology Manual, 6th ed. Star Publishing Co., Belmont, CA.
10. MacFaddin JF. 2000. Biochemical Tests for Identification of Medical Bacteria, 3rd ed. Lippincott Williams & Wilkins, Philadelphia, PA.
11. Engelkirk PG, Duben-Engelkirk J, Dowell VRJr. 1992. Principles and Practice of Clinical Anaerobic Bacteriology. Star Publishing Co., Belmont, CA.
12. Jousimies-Somer HR, Summanen P, Citron DM, Baron EJ, Wexler HM, Finegold SM. 2002. Wadsworth-KTL Anaerobic Bacteriology Manual, 6th ed. Star Publishing Co., Belmont, CA.
13. MacFaddin JF. 2000. Biochemical Tests for Identification of Medical Bacteria, 3rd ed. Lippincott Williams & Wilkins, Philadelphia, PA.
14. Engelkirk PG, Duben-Engelkirk J, Dowell VRJr. 1992. Principles and Practice of Clinical Anaerobic Bacteriology. Star Publishing Co., Belmont, CA.
15. Jousimies-Somer HR, Summanen P, Citron DM, Baron EJ, Wexler HM, Finegold SM. 2002. Wadsworth-KTL Anaerobic Bacteriology Manual, 6th ed. Star Publishing Co., Belmont, CA.
16. MacFaddin JF. 2000. Biochemical Tests for Identification of Medical Bacteria, 3rd ed. Lippincott Williams & Wilkins, Philadelphia, PA.
17. Wideman PA, Vargo VL, Citrobaum D, Finegold SM. 1976. Evaluation of the sodium polyanethol sulfonate disk test for the identification of Peptostreptococcus anaerobius. J Clin Microbiol 4:330333.
18. Engelkirk PG, Duben-Engelkirk J, Dowell VRJr. 1992. Principles and Practice of Clinical Anaerobic Bacteriology. Star Publishing Co., Belmont, CA.
19. Jousimies-Somer HR, Summanen P, Citron DM, Baron EJ, Wexler HM, Finegold SM. 2002. Wadsworth-KTL Anaerobic Bacteriology Manual, 6th ed. Star Publishing Co., Belmont, CA.
20. MacFaddin JF. 2000. Biochemical Tests for Identification of Medical Bacteria, 3rd ed. Lippincott Williams & Wilkins, Philadelphia, PA.
21. Jousimies-Somer HR, Summanen P, Citron DM, Baron EJ, Wexler HM, Finegold SM. 2002. Wadsworth-KTL Anaerobic Bacteriology Manual, 6th ed. Star Publishing Co., Belmont, CA.
22. Shah HN, Bonnett R, Mateen B, Williams RAD. 1979. The porphyrin pigmentation of subspecies of Bacteroides melaninogenicus. Biochem J 180:4550.
23. Slots J, Reynolds HS. 1982. Long-wave UV light fluorescence for identification of black-pigmented Bacteroides spp. J Clin Microbiol 16:11481151.
24. Engelkirk PG, Duben-Engelkirk J, Dowell VRJr. 1992. Principles and Practice of Clinical Anaerobic Bacteriology. Star Publishing Co., Belmont, CA.
25. Jousimies-Somer HR, Summanen P, Citron DM, Baron EJ, Wexler HM, Finegold SM. 2002. Wadsworth-KTL Anaerobic Bacteriology Manual, 6th ed. Star Publishing Co., Belmont, CA.
26. MacFaddin JF. 2000. Biochemical Tests for Identification of Medical Bacteria, 3rd ed. Lippincott Williams & Wilkins, Philadelphia, PA.
27. Engelkirk PG, Duben-Engelkirk J, Dowell VRJr. 1992. Principles and Practice of Clinical Anaerobic Bacteriology. Star Publishing Co., Belmont, CA.
28. Jousimies-Somer HR, Summanen P, Citron DM, Baron EJ, Wexler HM, Finegold SM. 2002. Wadsworth-KTL Anaerobic Bacteriology Manual, 6th ed. Star Publishing Co., Belmont, CA.
29. MacFaddin JF. 2000. Biochemical Tests for Identification of Medical Bacteria, 3rd ed. Lippincott Williams & Wilkins, Philadelphia, PA.
30. Engelkirk PG, Duben-Engelkirk J, Dowell VRJr. 1992. Principles and Practice of Clinical Anaerobic Bacteriology. Star Publishing Co., Belmont, CA.
31. Jousimies-Somer HR, Summanen P, Citron DM, Baron EJ, Wexler HM, Finegold SM. 2002. Wadsworth-KTL Anaerobic Bacteriology Manual, 6th ed. Star Publishing Co., Belmont, CA.
32. MacFaddin JF. 2000. Biochemical Tests for Identification of Medical Bacteria, 3rd ed. Lippincott Williams & Wilkins, Philadelphia, PA.
33. Shah HN, Bonnett R, Mateen B, Williams RAD. 1979. The porphyrin pigmentation of subspecies of Bacteroides melaninogenicus. Biochem J 180:4550.
34. Engelkirk PG, Duben-Engelkirk J, Dowell VRJr. 1992. Principles and Practice of Clinical Anaerobic Bacteriology. Star Publishing Co., Belmont, CA.
35. Jousimies-Somer HR, Summanen P, Citron DM, Baron EJ, Wexler HM, Finegold SM. 2002. Wadsworth-KTL Anaerobic Bacteriology Manual, 6th ed. Star Publishing Co., Belmont, CA.
36. MacFaddin JF. 2000. Biochemical Tests for Identification of Medical Bacteria, 3rd ed. Lippincott Williams & Wilkins, Philadelphia, PA.

Tables

Generic image for table
Table 4.6.5–1

Identification by means of special-potency antimicrobial agent disks

Citation: Hall G, Summanen P. 2016. Rapid Disk, Spot Tests, and Other Rapid or Primary Methods for the Identification of Anaerobes, p 4.6.1.1-4.6.13.5. In Leber A (ed), Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch4.6
Generic image for table
Table 4.6.8–1

Fluorescence of anaerobic bacteria

Citation: Hall G, Summanen P. 2016. Rapid Disk, Spot Tests, and Other Rapid or Primary Methods for the Identification of Anaerobes, p 4.6.1.1-4.6.13.5. In Leber A (ed), Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch4.6
Generic image for table
Summary of tests used for the rapid identification of anaerobesa

Summary of tests used for the rapid identification of anaerobes

Citation: Hall G, Summanen P. 2016. Rapid Disk, Spot Tests, and Other Rapid or Primary Methods for the Identification of Anaerobes, p 4.6.1.1-4.6.13.5. In Leber A (ed), Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch4.6

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