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Chapter 5.1 : Disk Diffusion Test

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Abstract:

A standardized inoculum of bacteria is swabbed onto the surface of a Mueller-Hinton agar (MHA) plate. Filter paper disks impregnated with antimicrobial agents are placed on the agar. After overnight incubation, the diameter of the zone of inhibition is measured around each disk. By referring to the tables in the CLSI standard ( ), a qualitative report of susceptible, intermediate, or resistant is obtained.

Citation: Leber A. 2016. Disk Diffusion Test, p 5.1.1-5.1.16. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch5.1
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Disk Diffusion Susceptibility Testing Quality Control

Disk Diffusion Susceptibility Testing Quality Control

Citation: Leber A. 2016. Disk Diffusion Test, p 5.1.1-5.1.16. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch5.1
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References

/content/book/10.1128/9781555818814.chap5.1
1. CLSI. 2015. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Fifth Informational Supplement. CLSI document M100-S25. CLSI, Wayne, PA.
2. CLSI. 2015. Performance Standards for Antimicrobial Disk Susceptibility Tests. Approved standard, 12th ed. CLSI document M02-A12. CLSI, Wayne, PA.
3. CLSI. 2015. Methods for Dilution and Disk Susceptibility Testing of Infrequently Isolated or Fastidious Bacteria. Approved guideline, 3rd ed. CLSI document M45-A3. CLSI, Wayne, PA.
4. Barry AL, Coyle MD, Thornsberry C, Gerlach EH, Hawkinson RW. 1979. Methods of measuring zones of inhibition with Bauer-Kirby disk susceptibility test. J Clin Microbiol 10:885889.
5. Bauer AW, Kirby WMM, Sherris JC, Turck M. 1966. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 45:493496.
6. Jorgensen JH, Pfaller MA (ed). 2015. Manual of Clinical Microbiology, 11th ed, p 11691389. ASM Press, Washington, DC.
1. CLSI. 2015. Performance Standards for Antimicrobial Disk Susceptibility Tests. Approved standard, 12th ed. CLSI document M02-A12. CLSI, Wayne, PA.
2. CLSI. 2015. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Fifth Informational Supplement. CLSI document M100-S25. CLSI, Wayne, PA.
1. CLSI. 2015. Performance Standards for Antimicrobial Disk Susceptibility Tests. Approved standard, 12th ed. CLSI document M02-A12. CLSI, Wayne, PA.
2. CLSI. 2015. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Fifth Informational Supplement. CLSI document M100-S25. CLSI, Wayne, PA.
1. CLSI. 2015. Performance Standards for Antimicrobial Disk Susceptibility Tests. Approved standard, 12th ed. CLSI document M02-A12. CLSI, Wayne, PA.
2. CLSI. 2015. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Fifth Informational Supplement. CLSI document M100-S25. CLSI, Wayne, PA.
1. CLSI. 2015. Performance Standards for Antimicrobial Disk Susceptibility Tests. Approved standard, 12th ed. CLSI document M02-A12. CLSI, Wayne, PA.
2. CLSI. 2015. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Fifth Informational Supplement. CLSI document M100-S25. CLSI, Wayne, PA.
1. CLSI. 2015. Performance Standards for Antimicrobial Disk Susceptibility Tests. Approved standard, 12th ed. CLSI document M02-A12. CLSI, Wayne, PA.
2. CLSI. 2015. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Fifth Informational Supplement. CLSI document M100-S25. CLSI, Wayne, PA.
1. CLSI. 2015. Performance Standards for Antimicrobial Disk Susceptibility Tests. Approved standard, 12th ed. CLSI document M02-A12. CLSI, Wayne, PA.
2. CLSI. 2015. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Fifth Informational Supplement. CLSI document M100-S25. CLSI, Wayne, PA.

Tables

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Untitled

Citation: Leber A. 2016. Disk Diffusion Test, p 5.1.1-5.1.16. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch5.1
Generic image for table
Untitled

Citation: Leber A. 2016. Disk Diffusion Test, p 5.1.1-5.1.16. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch5.1
Generic image for table
Quick reference list for performing disk diffusion testsa

Quick reference list for performing disk diffusion tests

Citation: Leber A. 2016. Disk Diffusion Test, p 5.1.1-5.1.16. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch5.1

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