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Chapter 5.11 : Agar Screen Test To Detect Vancomycin Resistance in spp.

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Agar Screen Test To Detect Vancomycin Resistance in spp., Page 1 of 2

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Abstract:

The vancomycin agar screen is used to detect vancomycin-resistant enterococcal colonies that have been isolated from clinical or surveillance cultures. A standard number of bacteria is inoculated onto BHI agar containing 6 μg of vancomycin per ml. Following incubation, the appearance of growth indicates that the enterococcal isolate is likely to be resistant to vancomycin. MIC and species identification tests are subsequently required to determine if the isolate is a true vancomycin-resistant enterococcus (VRE) ( ).

Citation: Leber A. 2016. Agar Screen Test To Detect Vancomycin Resistance in spp., p 5.11.1-5.11.4. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch5.11
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Enterococcus Vancomycin Agar Screen Test QC

Vancomycin Agar Screen Test QC

Citation: Leber A. 2016. Agar Screen Test To Detect Vancomycin Resistance in spp., p 5.11.1-5.11.4. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch5.11
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References

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1. CLSI. 2015. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically, 10th ed. Approved standard M07-A10. CLSI, Wayne, PA.
2. Swenson JM, Patel JB, Jorgensen JH. 2011. Special phenotypic methods for detecting antimicrobial resistance, p 1155–1179. In Versalovic J, Carroll KC, Funke G, Jorgensen JH, Landry ML, Warnock DW (ed), Manual of Clinical Microbiology, 10th ed. ASM Press, Washington, DC.
3. Sujatha S, Praharaj I. 19 June 2012. Glycopeptide resistance in gram-positive cocci: a review. Interdiscip Perspect Infect Dis doi:10.1155/2012/781679.
4. Jenney A, Franklin C, Liolios L, Spelman D. 2000. Enterococcus durans VanB. J Antimicrob Chemother 46:515.
5. Jenkins SG, Schuetz AN. 2012. Current concepts in laboratory testing to guide antimicrobial therapy. Mayo Clin Proc 87:290308.
6. Hindler JF. 2010. Agar screen test to detect vancomycin resistance in Enterococcus spp. In Garcia LS (ed), Clinical Microbiology Procedures Handbook, 3 ed. ASM Press, Washington, DC.
7. Cetinkaya Y, Falk P, Mayhall CG. 2000. Vancomycin-resistant enterococci. Clin Microbiol Rev 13:686707.

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