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Chapter 5.2 : B M T

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Abstract:

The broth microdilution MIC method is used to measure (semiquantitatively) the activity of an antimicrobial agent against a bacterial isolate. A sterile multiwell plastic tray containing various concentrations of antimicrobial agents is inoculated with a standardized number of test bacteria. After overnight incubation at 35 ± 2°C, the MIC is determined by observing the lowest concentration of an antimicrobial agent which will inhibit visible growth of the bacterium. MICs obtained are interpreted as susceptible, intermediate, or resistant, based on the criteria listed in the CLSI MIC standard ( ).

Citation: Leber A. 2016. B M T, p 5.2.1.1-5.2.2.10. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch5.2
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Broth Microdilution QC

Broth Microdilution QC

Citation: Leber A. 2016. B M T, p 5.2.1.1-5.2.2.10. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch5.2
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Anaerobe Broth Microdilution QC

Anaerobe Broth Microdilution QC

Citation: Leber A. 2016. B M T, p 5.2.1.1-5.2.2.10. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch5.2
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References

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1. CLSI. 2015. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically, 10th ed. Approved standard M07-A10. CLSI, Wayne, PA.
2. CLSI. 2015. Performance Standards for Antimicrobial Susceptibility Testing, 25th ed. Informational supplement M100-S25. CLSI, Wayne, PA.
3. CLSI. 2015. Methods for Dilution and Disk Susceptibility Testing of Infrequently Isolated or Fastidious Bacteria, 3rd ed. Approved guideline M45-A3. CLSI, Wayne, PA.
4. Hindler JF, Tamashiro L. 2010. Broth microdilution MIC test. In Garcia LS (ed), Clinical Microbiology Procedures Handbook, 3 ed. ASM Press, Washington, DC.
5. Barry AL, Braun LE. 1981. Reader error in determining minimal inhibitory concentrations with microdilution susceptibility test panels. J Clin Microbiol 13:228230.
6. ISO. 2006. ISO 20776-1:2006. Clinical Laboratory Testing and In Vitro Diagnostic Test Systems—Susceptibility Testing of Infectious Agents and Evaluation of Performance of Antimicrobial Susceptibility Test Devices—Part 1: Reference Method for Testing the In Vitro Activity of Antimicrobial Agents against Rapidly Growing Aerobic Bacteria Involved in Infectious Diseases. ISO, Geneva, Switzerland.
7. Jorgensen JH, Ferraro MJ. 2000. Antimicrobial susceptibility testing: special needs for fastidious organisms and difficult-to-detect resistance mechanisms. Clin Infect Dis 30:799808.
8. Jorgensen JH, Hindler JF. 2007. New consensus guidelines from the Clinical and Laboratory Standards Institute for antimicrobial susceptibility testing of infrequently isolated or fastidious bacteria. Clin Infect Dis 44:280286.
9. Jorgensen JH Turnidge JD. 2015. Susceptibility test methods: dilution and disk diffusing methods, p 1253–1273. In Jorgensen JH, Pfaller M, Carroll KC, Funke G, Landry ML, Richter SS, Warnock DW (ed), Manual of Clinical Microbiology, 11th ed. ASM Press, Washington, DC.
1. CLSI. 2015. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically, 10th ed. Approved standard M07-A10. CLSI, Wayne, PA.
2. CLSI. 2015. Performance Standards for Antimicrobial Susceptibility Testing, 25th ed. Informational supplement M100-S25. CLSI, Wayne, PA.
3. CLSI. 2015. Methods for Antimicrobial Dilution and Disk Susceptibility Testing of Infrequently Isolated or Fastidious Bacteria, 3rd ed. Approved guideline M45-A3. CLSI, Wayne, PA.
1. CLSI. 2015. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically, 10th ed. Approved standard M07-A10. CLSI, Wayne, PA.
2. CLSI. 2015. Performance Standards for Antimicrobial Susceptibility Testing, 25th ed. Informational supplement M100-S25. CLSI, Wayne, PA.
1. CLSI. 2015. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically, 10th ed. Approved standard M07-A10. CLSI, Wayne, PA.
2. CLSI. 2015. Performance Standards for Antimicrobial Susceptibility Testing, 25th ed. Informational supplement M100-S25. CLSI, Wayne, PA.
3. CLSI. 2015. Methods for Antimicrobial Dilution and Disk Susceptibility Testing of Infrequently Isolated or Fastidious Bacteria, 3rd ed. Approved guideline M45-A3. CLSI, Wayne, PA.
1. CLSI. 2015. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically, 10th ed. Approved standard M7-A10. CLSI, Wayne, PA.
2. CLSI. 2015. Performance Standards for Antimicrobial Susceptibility Testing, 25th ed. Informational supplement M100-S25. CLSI, Wayne, PA.
1. CLSI. 2015. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically, 10th ed. Approved standard M07-A10. CLSI, Wayne, PA.
1. CLSI. 2015. Performance Standards for Antimicrobial Susceptibility Testing, 25th ed. Informational supplement M100-S25. CLSI, Wayne, PA.
2. CLSI. 2012. Methods for Antimicrobial Susceptibility Testing of Anaerobic Bacteria. Approved standard M11-A8. CLSI, Wayne, PA.
3. Roe-Carpenter D. 2010. Broth microdilution MIC test for anaerobic bacteria. In Garcia LS (ed), Clinical Microbiology Procedures Handbook, 3 ed. ASM Press, Washington, DC.
24. Schuetz AN, Hecht DW. 2015. Susceptibility test methods: anaerobic bacteria, p 1342–1355. In Jorgensen JH, Pfaller MA, Carroll KC, Funke G, Landry ML, Richter SS, Warnock DW (ed), Manual of Clinical Microbiology, 11th ed. ASM Press, Washington, DC.
25. Finegold SM, George WL. 1989. Anaerobic Infections in Humans. Academic Press, Inc, San Diego, CA.
26. Goldstein EJ, Citron DM, Goldman PJ, Goldman RJ. 2008. National hospital survey of anaerobic culture and susceptibility methods: III. Anaerobe 14:6872.
27. Holdeman LV, Cato EP, Moore WEC (ed). 1987. Anaerobe Laboratory Manual, 4th ed. Virginia Polytechnic Institute and State University, Blacksburg, VA.
28. Jousimies-Somer HR, Summanen P, Citron DM, Baron EJ, Wexler HM, Finegold SM. 2002. Wadsworth-KTL Anaerobic Bacteriology Manual, 6th ed. Star Publishing Co, Belmont, CA.
1. Jousimies-Somer HR, Summanen P, Citron DM, Baron EJ, Wexler HM, Finegold SM. 2002. Wadsworth-KTL Anaerobic Bacteriology Manual, 6th ed. Star Publishing Co, Belmont, CA.
2. CLSI. 2012. Methods for Antimicrobial Susceptibility Testing of Anaerobic Bacteria. Approved standard M11-A8. CLSI, Wayne, PA.

Tables

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Citation: Leber A. 2016. B M T, p 5.2.1.1-5.2.2.10. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch5.2
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Untitled

Citation: Leber A. 2016. B M T, p 5.2.1.1-5.2.2.10. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch5.2
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Quick reference list for performing broth microdilution MIC tests (1, 2)a

Quick reference list for performing broth microdilution MIC tests ( )

Citation: Leber A. 2016. B M T, p 5.2.1.1-5.2.2.10. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch5.2
Generic image for table
Untitled

Citation: Leber A. 2016. B M T, p 5.2.1.1-5.2.2.10. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch5.2
Generic image for table
Untitled

Citation: Leber A. 2016. B M T, p 5.2.1.1-5.2.2.10. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch5.2
Generic image for table
Untitled

Citation: Leber A. 2016. B M T, p 5.2.1.1-5.2.2.10. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch5.2

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