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Chapter 5.20 : P R M R U A S T

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P R M R U A S T, Page 1 of 2

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Abstract:

McFarland turbidity standards are used to standardize the approximate number of bacteria in a liquid suspension by visually comparing the turbidity of a test suspension with the turbidity of a McFarland standard. McFarland standards are prepared by adding barium chloride to sulfuric acid to obtain a barium sulfate precipitate. By adjusting the volumes of these two reagents, standards of varying degrees of turbidity can be prepared to represent several different concentrations of bacteria. The standard most commonly used in the clinical microbiology laboratory for routine antimicrobial susceptibility tests ( ) is 0.5, which represents 1.5 × 10 (generally, range is 1.0 × 10 to 2.0 × 10) bacteria/ml. McFarland standards are commercially available from several sources. As an alternative to the traditional barium sulfate standards, McFarland standards prepared from latex particles have recently become commercially available. This procedure is based on material presented in the previous edition of this manual ( ).

Citation: Leber A. 2016. P R M R U A S T, p 5.20.1.1-5.20.3.10. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch5.20
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References

/content/book/10.1128/9781555818814.chap5.20
1. CLSI. 2015. Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard, 12th ed. CLSI document M02-A12. CLSI, Wayne, PA.
2. CLSI. 2015. Methods for Dilution Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard, 10th ed. CLSI document M07-A10. CLSI, Wayne, PA.
3. Hindler JF, Hochstein L, Howell A. 2010. McFarland standards, p 5.14.1.1–5.14.1.4. In Garcia LS (ed), Clinical Microbiology Procedures Handbook, 3 ed. ASM Press, Washington, DC.
4. Barry AL. 1976. The Antimicrobic Susceptibility Test: Principles and Practices, p 7274. Lea and Febiger, Philadelphia, PA.
5. McFarland J. 1907. Nephelometer: an instrument for estimating the number of bacteria in suspensions used for calculating the opsonic index and for vaccines. JAMA 14:11761178.
6. Washington JA, Warren E, Karlson AG. 1972. Stability of barium sulfate turbidity standards. Appl Microbiol 24:1013.
1. CLSI. 2015. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard, 10th ed. CLSI document M07-A10. CLSI, Wayne, PA.
2. CLSI. 2015. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Fifth Informational Supplement. CLSI document M100-S25. CLSI, Wayne, PA.
3. Hindler JF, Hochstein L, Howell A. 2010. Antimicrobial stock solutions, p 5.14.2.1–5.14.2.7. In Garcia LS (ed), Clinical Microbiology Procedures Handbook, 3 ed. ASM Press, Washington, DC.
4. Shungu DL. 1996. Chemical and physical properties of antibiotics: preparation and control of antibiotic susceptibility disks and other devices containing antibiotics, p 766–792. In Lorian V (ed), Antibiotics in Laboratory Medicine, 4th ed. The Williams and Wilkins Co, Baltimore, MD.
1. Costilow RN. 1981. Biophysical factors in growth, p 67–69. In Gerhardt P (ed), Manual of Methods for General Bacteriology. American Society for Microbiology, Washington, DC.
1. CLSI. 2015. Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard, 12th ed. CLSI document M02-A12. CLSI, Wayne, PA.
2. CLSI. 2015. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard, 10th ed. CLSI document M07-A10. CLSI, Wayne, PA.
3. Hindler JF, Hochstein L, Howell A. 2010. Preparation of agar and broth media used in routine antimicrobial susceptibility tests, p 5.14.3.1–5.14.3.10. In Garcia LS (ed), Clinical Microbiology Procedures Handbook, 3 ed. ASM Press, Washington, DC.
4. CLSI. 2015. Protocols for Evaluating Dehydrated Mueller-Hinton Agar; Approved Standard, 2nd ed. CLSI document M06-A2. CLSI, Wayne, PA.
5. NCCLS. 2001. Evaluation of Lots of Mueller-Hinton Broth for Antimicrobial Susceptibility Testing; Proposed guideline. NCCLS document M32-P. NCCLS, Wayne, PA.
17. CLSI. 2015. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard, 10th ed. CLSI document M07-A10. CLSI, Wayne, PA.
1. CLSI. 2015. Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard, 12th ed. CLSI document M02-A12. CLSI, Wayne, PA.
2. CLSI. 2015. Methods for Dilution Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard, 10th ed. CLSI document M07-A10. CLSI, Wayne, PA.

Tables

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Citation: Leber A. 2016. P R M R U A S T, p 5.20.1.1-5.20.3.10. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch5.20
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Citation: Leber A. 2016. P R M R U A S T, p 5.20.1.1-5.20.3.10. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch5.20
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Untitled

Citation: Leber A. 2016. P R M R U A S T, p 5.20.1.1-5.20.3.10. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch5.20
Generic image for table
Untitled

Citation: Leber A. 2016. P R M R U A S T, p 5.20.1.1-5.20.3.10. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch5.20
Generic image for table
Untitled

Citation: Leber A. 2016. P R M R U A S T, p 5.20.1.1-5.20.3.10. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch5.20
Generic image for table
Untitled

Citation: Leber A. 2016. P R M R U A S T, p 5.20.1.1-5.20.3.10. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch5.20
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Antimicrobial Stock Solution QC

Antimicrobial Stock Solution QC

Citation: Leber A. 2016. P R M R U A S T, p 5.20.1.1-5.20.3.10. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch5.20
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Notes and QC parameters for mediaa

Notes and QC parameters for media

Citation: Leber A. 2016. P R M R U A S T, p 5.20.1.1-5.20.3.10. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch5.20

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