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Chapter 5.4 : A D MIC T

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Abstract:

The agar dilution MIC test is a quantitative method for the determination of activity of an antimicrobial agent against bacterial isolates. A series of agar plates, each containing a unique concentration of an antimicrobial agent, is inoculated with up to 36 isolates per plate. Following incubation using conditions appropriate for the test organisms, the MIC is determined by observing the lowest concentration of antimicrobial agent that inhibits visible growth of each test isolate. MICs obtained are interpreted as susceptible, intermediate, resistant, or nonsusceptible based on criteria defined by the CLSI ( ).

Citation: Leber A. 2016. A D MIC T, p 5.4.1.1-5.4.2.12. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch5.4
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Agar Dilution QC

Agar Dilution QC

Citation: Leber A. 2016. A D MIC T, p 5.4.1.1-5.4.2.12. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch5.4
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Image of Agar Dilution MIC Worksheet
Agar Dilution MIC Worksheet

Agar Dilution MIC Worksheet

Citation: Leber A. 2016. A D MIC T, p 5.4.1.1-5.4.2.12. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch5.4
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Image of Agar Dilution QC
Agar Dilution QC

Agar Dilution QC

Citation: Leber A. 2016. A D MIC T, p 5.4.1.1-5.4.2.12. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch5.4
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Image of Agar Dilution MIC Worksheet
Agar Dilution MIC Worksheet

Agar Dilution MIC Worksheet

Citation: Leber A. 2016. A D MIC T, p 5.4.1.1-5.4.2.12. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch5.4
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References

/content/book/10.1128/9781555818814.chap5.4
1. CLSI. 2015. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-fifth Informational Supplement. CLSI M100-S25. CLSI, Wayne, PA.
2. CLSI. 2010. Methods for Antimicrobial Dilution and Disk Susceptibility Testing of Infrequently Isolated or Fastidious Bacteria; Approved Guideline – Second Edition. CLSI M45-A2. CLSI, Wayne, PA.
3. CLSI. 2015. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically; Approved Standard – Tenth Edition. CLSI M07-A10. CLSI, Wayne, PA.
4. CLSI. 2008. Development of In Vitro Susceptibility Testing Criteria and Quality Control Parameters; Approved Guideline – Third Edition. CLSI M23-A3. CLSI, Wayne, PA.
5. European Committee for Antimicrobial Susceptibility Testing. 2000. Determination of minimum inhibitory concentrations (MICs) of antibacterial agents by agar dilution. Clin Microbiol Infect 6:509515.
1. CLSI. 2015. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically; Approved Standard – Tenth Edition. CLSI M07-A10. CLSI, Wayne, PA.
2. CLSI. 2015. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-fifth Informational Supplement. CLSI M100-S25. CLSI, Wayne, PA.
3. CLSI. 2008. Development of In Vitro Susceptibility Testing Criteria and Quality Control Parameters; Approved Guideline – Third Edition. CLSI M23-A3. CLSI, Wayne, PA.
1. CLSI. 2015. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-fifth Informational Supplement. CLSI M100-S25. CLSI, Wayne, PA.
1. CLSI. 2010. Methods for Antimicrobial Dilution and Disk Susceptibility Testing of Infrequently Isolated or Fastidious Bacteria; Approved Guideline – Second Edition. CLSI M45-A2. CLSI, Wayne, PA.
1. CLSI. 2015. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically; Approved Standard – Tenth Edition. CLSI M07-A10. CLSI, Wayne, PA.
1. CLSI. 2015. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-fifth Informational Supplement. CLSI M100-S25. CLSI, Wayne, PA.
2. CLSI. 2012. Methods for Antimicrobial Susceptibility Testing of Anaerobic Bacteria; Approved Standard – Eighth Edition. Approved standard M11-A8. CLSI, Wayne, PA.
3. Roe-Carpenter D. 2010. Agar dilution MIC test for anaerobic bacteria. In Garcia LS (ed), Clinical Microbiology Procedures Handbook, 3rd ed. ASM Press, Washington, DC.
4. Jousimies-Somer HR, Summanen P, Citron DM, Baron EJ, Wexler HM, Finegold SM. 2002. Wadsworth-KTL Anaerobic Bacteriology Manual, 6th ed. Star Publishing Co., Belmont, CA.
16. Citron DM, Hecht DW. 2011. Susceptibility test methods: anaerobic bacteria, p 1204–1214. In Versalovic J, Jorgensen JH, Landry ML, Warnock DW (ed), Manual of Clinical Microbiology, 10th ed. ASM Press, Washington, DC.
17. Finegold SM, George WL. 1989. Anaerobic Infections in Humans. Academic Press, Inc., San Diego, CA.
18. Holdeman LV, Cato EP, Moore WEC (ed). 1977. Anaerobe Laboratory Manual, 4th ed. Virginia Polytechnic Institute and State University, Blacksburg, VA.
1. Jousimies-Somer HR, Summanen P, Citron DM, Baron EJ, Wexler HM, Finegold SM. 2002. Wadsworth-KTL Anaerobic Bacteriology Manual, 6th ed. Star Publishing Co., Belmont, CA.
2. CLSI. 2012. Methods for Antimicrobial Susceptibility Testing of Anaerobic Bacteria; Approved Standard – Eighth Edition. Approved standard M11-A8. CLSI, Wayne, PA.

Tables

Generic image for table
Untitled

Citation: Leber A. 2016. A D MIC T, p 5.4.1.1-5.4.2.12. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch5.4
Generic image for table
Quick Reference Guide for Performing Agar Dilution MIC Testsa

Quick Reference Guide for Performing Agar Dilution MIC Tests

Citation: Leber A. 2016. A D MIC T, p 5.4.1.1-5.4.2.12. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch5.4
Generic image for table
Volumes of components required for preparation of agar dilution plates when using round or square petri plates

Volumes of components required for preparation of agar dilution plates when using round or square petri plates

Citation: Leber A. 2016. A D MIC T, p 5.4.1.1-5.4.2.12. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch5.4
Generic image for table
Patterns for arrangement of isolates in replicator

Patterns for arrangement of isolates in replicator

Citation: Leber A. 2016. A D MIC T, p 5.4.1.1-5.4.2.12. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch5.4
Generic image for table
Preparation of antimicrobial dilutions from stock solutionsa

Preparation of antimicrobial dilutions from stock solutions

Citation: Leber A. 2016. A D MIC T, p 5.4.1.1-5.4.2.12. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch5.4
Generic image for table
Untitled

Citation: Leber A. 2016. A D MIC T, p 5.4.1.1-5.4.2.12. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch5.4
Generic image for table
Preparation of antimicrobial dilutions from stock solutionsa

Preparation of antimicrobial dilutions from stock solutions

Citation: Leber A. 2016. A D MIC T, p 5.4.1.1-5.4.2.12. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch5.4
Generic image for table
Volumes of components required for preparation of agar dilution plates when using round or square petri plates

Volumes of components required for preparation of agar dilution plates when using round or square petri plates

Citation: Leber A. 2016. A D MIC T, p 5.4.1.1-5.4.2.12. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch5.4
Generic image for table
Patterns for arrangement of isolates in replicator

Patterns for arrangement of isolates in replicator

Citation: Leber A. 2016. A D MIC T, p 5.4.1.1-5.4.2.12. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch5.4

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