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Chapter 5.6 : Oxacillin Salt-Agar Screen Test To Detect Oxacillin (Methicillin)-Resistant

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Oxacillin Salt-Agar Screen Test To Detect Oxacillin (Methicillin)-Resistant , Page 1 of 2

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Abstract:

A standard number of bacteria is inoculated onto Mueller-Hinton agar containing 6 μg of oxacillin per ml and 4% NaCl. Following overnight incubation, the appearance of growth indicates that the isolate is resistant to oxacillin, all other penicillins, carbapenems, beta-lactam–beta-lactamase inhibitor combinations, and cephems with the exception of ceftaroline ( ).

Citation: Leber A. 2016. Oxacillin Salt-Agar Screen Test To Detect Oxacillin (Methicillin)-Resistant , p 5.6.1-5.6.4. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch5.6
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Staphylococcus aureus Oxacillin Agar Screen Test QC

Oxacillin Agar Screen Test QC

Citation: Leber A. 2016. Oxacillin Salt-Agar Screen Test To Detect Oxacillin (Methicillin)-Resistant , p 5.6.1-5.6.4. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch5.6
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References

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1. CLSI. 2015. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically, 10th ed. Approved Standard. M07-A10. CLSI, Wayne, PA.
2. CLSI. 2015. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Fifth Informational Supplement. Approved standard M100-S25. CLSI, Wayne, PA.
3. Swenson JM, Williams PP, Killgore G, O’Hara CM, Tenover FC. 2001. Performance of eight methods, including two new rapid methods, for detection of oxacillin resistance in a challenge set of Staphylococcus aureus organisms. J Clin Microbiol 39:37853788.
4. Baddour MM, Abuelkheir MM, Fatani AJ. 2007. Comparison of mecA polymerase chain reaction with phenotypic methods for the detection of methicillin-resistant Staphylococcus aureus. Curr Microbiol 55:473479.
5. Bethel CD, Boonlayangoor S. 2010. Oxacillin Salt-Agar Screen Test To Detect Oxacillin (Methicillin)-Resistant Staphylococcus aureus. In Garcia LS (ed), Clinical Microbiology Procedures Handbook, 3 ed. ASM Press, Washington, DC.
6. Datta P, Gulati N, Rani VH, Bala K, Chandler J, Gupta V. 2011. Evaluation of various methods for the detection of methicillin-resistant Staphylococcus aureus strains and susceptibility patterns. J Med Microbiol 60:16131616.
7. Jenkins SG, Schuetz AN. 2012. Current concepts in laboratory testing to guide antimicrobial therapy. Mayo Clin Proc 87:290308.
8. Swenson JM, Lonsway D, McAllister S, Thompson A, Jevitt L, Zhu W, Patel JB. 2007. Detection of mecA-mediated resistance using reference and commercial testing methods in a collection of Staphylococcus aureus expressing borderline oxacillin MICs. Diagn Microbiol Infect Dis 58:3339.
9. Swenson JM, Spargo J, Tenover FC, Ferraro MJ. 2001. Optimal inoculation methods and quality control for the NCCLS oxacillin agar screen test for detection of oxacillin resistance in Staphylococcus aureus. J Clin Microbiol 39:37813784.
10. Yamada K, Inuzuka K, Tatsumi N, Sanzen I, Ohkura T, Okamoto A, Hasegawa T, Ohta M. 2010. Evaluation of selection media for the detection of borderline MRSA. J Infect Chemother 16(2):1924.

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