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Chapter 7.4 : Liquid Media Used for Isolation
The use of liquid medium for isolation of mycobacteria and drug susceptibility testing of Mycobacterium tuberculosis (MTB) was first introduced in 1980 as the BACTEC 460 radiometric system (Becton Dickinson Diagnostic Systems, Sparks, MD). This system used 14C-labeled palmitic acid for the detection of growth. Scores of publications validated an early detection of growth and an increase in the number of positive cultures with using this liquid medium. However, because of the concerns with the use of radioactive material and its disposal, a nonradiometric BACTEC MGIT 960 System was developed (Becton Dickinson Diagnostic Systems, Sparks, MD). The BACTEC 460 radiometric System was discontinued in August 2012.