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Chapter 7.7 : S T L M M

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Abstract:

The BACTEC MGIT 960 SIRE kit is a nonradiometric antimicrobial susceptibility (AST) system for testing from isolated culture. It provides susceptibility results for streptomycin (STR), isoniazid (INH), rifampin (RIF), and ethambutol (EMB) in approximately the same time frame as the BACTEC 460TB system. The BBL MGIT 7-ml mycobacteria growth indicator tube (MGIT tube) contains a modified Middlebrook 7H9 broth, which supports the growth and detection of mycobacteria. The MGIT tube contains a fluorescent compound embedded in silicone on the bottom of a 16- by 100-mm round-bottom tube. The fluorescent compound is sensitive to the presence of oxygen dissolved in the broth. The initial concentration of dissolved oxygen quenches the emission of fluorescence from the compound, and little fluorescence can be detected. Later, actively growing and respiring microorganisms consume the oxygen, which allows the compound to fluoresce.

Citation: Leber A. 2016. S T L M M, p 7.7.1.1-7.7.2.4. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch7.7
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References

/content/book/10.1128/9781555818814.chap7.7
1. Barenfanger J. 1993. Making your lab safe against multidrug resistant Mycobacterium tuberculosis. Clin Microbiol Newsl 15:7680.
2. Becton Dickinson Diagnostic Systems. Data on file.
3. CLSI. 2011. Susceptibility testing of Mycobacteria, Nocardia, and Other Aerobic Actinomycetes, 2nd ed., vol. 31, no. 5. M24-A2. CLSI, Wayne, PA.
4. Siddiqi SH, Rüsch-Gerdes S. 2006. MGIT Procedure Manual. Foundation for Innovative New Diagnostics (FIND), Geneva, Switzerland.
1. LaBombardi VJ, Lukowski C. 1997. Antitubercular susceptibility testing using the ESP culture system II, abstr Y75. Abstr Gen Meet Am Soc Microbiol. American Society for Microbiology, Washington, DC.
2. VJ. LaBombardi 2002. Comparison of the ESP and Bactec Systems for testing susceptibilities of Mycobacterium tuberculosis complex isolates to pyrazinamide. J Clin Microbiol 40:22382239
3. Thermo Fisher Scientific. 2013. VersaTREK Myco Susceptibility Kit, Package Insert. Thermo Fisher Scientific, Cleveland, OH.
4. Thermo Fisher Scientific. 2013. VersaTREK Myco PZA Kit, Package Insert. Thermo Fisher Scientific, Cleveland, OH.

Tables

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Citation: Leber A. 2016. S T L M M, p 7.7.1.1-7.7.2.4. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch7.7
Generic image for table
Untitled

Citation: Leber A. 2016. S T L M M, p 7.7.1.1-7.7.2.4. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch7.7
Generic image for table
Untitled

Citation: Leber A. 2016. S T L M M, p 7.7.1.1-7.7.2.4. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch7.7
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Table 7.7.2–1

Reagent formulations upon rehydration

Citation: Leber A. 2016. S T L M M, p 7.7.1.1-7.7.2.4. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch7.7
Generic image for table
Table 7.7.2–2

Drug concentrations

Citation: Leber A. 2016. S T L M M, p 7.7.1.1-7.7.2.4. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch7.7

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