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Chapter 7.8 : Susceptibility Testing by Agar Proportion Method for Complex

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Susceptibility Testing by Agar Proportion Method for Complex, Page 1 of 2

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Abstract:

Antimicrobial susceptibility testing should be performed on each patient’s initial complex isolate, in relapse or re-treatment cases, and when acquired drug resistance is suspected. The modified agar proportion method is used to determine the susceptibility of complex, indirectly from a pure culture. Quadrant plates are used that contain either Middlebrook 7H10 agar without antimicrobial agents (control plate) or 7H10 agar with antimicrobial agents at various concentrations (testing plates). Most of primary antituberculosis drugs (i.e., isoniazid [INH], ethambutol [EMB], and rifampin [RIF]) and second-line drugs can be tested with this method. Pyrazinamide (PZA), however, cannot be tested by this method.

Citation: Leber A. 2016. Susceptibility Testing by Agar Proportion Method for Complex, p 7.8.1-7.8.6. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch7.8
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References

/content/book/10.1128/9781555818814.chap7.8
1. CLSI. 2011. Susceptibility Testing of Mycobacteria, Nocardiae and Other Aerobic Actinomycetes; Approved Standard, 2nd ed. CLSI document M24-2A. CLSI, Wayne, PA.
2. Kent PT, Kubica GP. 1985. Public Health Mycobacteriology: A Guide for the Level III Laboratory. US Department of Health and Human Services, CDC, Atlanta, GA.
3. Cohn ML, Waggoner RF, McClatchy JK. 1968. The 7H11 medium for the cultivation of mycobacteria. Am Rev Respir Dis 98:295296.
4. World Health Organization. 2014. Companion Handbook to the WHO Guidelines for the Programmatic Management of Drug-Resistant Tuberculosis. WHO/HTM/TB/2014.11. World Health Organization, Geneva, Switzerland.

Tables

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Table 7.8–1

Recommended drug concentrations in Middlebrook 7H10 agar ( )

Citation: Leber A. 2016. Susceptibility Testing by Agar Proportion Method for Complex, p 7.8.1-7.8.6. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch7.8
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Guidelines for selection of the dilution of a specimen concentrate prior to inoculation of 7H10 medium for susceptibility testing using the direct method

Guidelines for selection of the dilution of a specimen concentrate prior to inoculation of 7H10 medium for susceptibility testing using the direct method

Citation: Leber A. 2016. Susceptibility Testing by Agar Proportion Method for Complex, p 7.8.1-7.8.6. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch7.8
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Untitled

Citation: Leber A. 2016. Susceptibility Testing by Agar Proportion Method for Complex, p 7.8.1-7.8.6. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch7.8

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