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Chapter 8.4 : Processing Specimens for Fungal Culture

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Processing Specimens for Fungal Culture, Page 1 of 2

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Abstract:

When a specimen is suspected to contain a fungal etiologic agent, it should be processed for fungal culture, regardless of direct microscopic findings. Recovery of fungal pathogens in culture provides definitive diagnosis of mycotic disease, identifies the etiologic agent of infection, and allows evaluation of susceptibility to antifungal agents. If there is insufficient material for both microscopy and culture, all of the specimen should be used for culture, since this is the more sensitive procedure for detection of fungi. Methods of specimen processing and culture are designed to retain the viability of the fungus and to obtain the maximum yield of organisms from clinical specimens. The choice of media for the isolation of fungi from clinical material is based primarily on the most likely species to be found in a particular site or under a recognized clinical condition. Selective media are included when other microorganisms, particularly bacteria, might also be present in the specimen. Specimens should be processed as soon as possible after receipt. Some specimens may require pretreatment prior to culture.

Citation: Leber A. 2016. Processing Specimens for Fungal Culture, p 8.4.1-8.4.6. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.4
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References

/content/book/10.1128/9781555818814.chap8.4
1. Snyder JW, RH. Atlas 2006. Handbook of Media for Clinical Microbiology, 2nd ed. CRC Press, Boca Raton, FL.
2. Evans EGV, MD. Richardson 1989. Medical Mycology–A Practical Approach. IRL Press at Oxford University Press, Oxford, United Kingdom.
3. MR. McGinnis 1980. Laboratory Handbook of Medical Mycology. Academic Press, Boca Raton, FL.
4. KL. McGowan 2011. Specimen collection, transport, and processing: mycology, p 1756–1766. In Versalovic J, Carroll KC, Funke G, Jorgensen JH, Landry ML, Warnock DW (ed), Manual of Clinical Microbiology, 10th ed, vol 2. ASM Press, Washington, DC.
5. Robinson BE, AA. Padhye 1988. Collection anbd transport of clinical specimens, p 11–32. In Wentworth BB (ed), Diagnostic Procedures for Mycotic and Parasitic Diseases, 7th ed. American Public Health Association, Washington, DC.
6. Snyder JW, Atlas RH, LaRocco MT. 2011. reagents, stains, and media: mycology, p 1767–1775. In Versalovic J, Carroll KC, Funke G, Jorgensen JH, Landry ML, Warnock DW (ed), Manual of Clinical Microbiology, 10th ed, vol 2. ASM Press, Washington, DC.

Tables

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Table 8.4–1

Media for the isolation and identification of fungi

Citation: Leber A. 2016. Processing Specimens for Fungal Culture, p 8.4.1-8.4.6. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.4

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