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Chapter 8.5 : Examination and Evaluation of Primary Cultures

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Examination and Evaluation of Primary Cultures, Page 1 of 2

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Abstract:

Citation: Leber A. 2016. Examination and Evaluation of Primary Cultures, p 8.5.1-8.5.10. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.5
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Figures

Image of Figure 8.5–1
Figure 8.5–1

Flowchart for evaluating a possible yeast species on primary culture. (A) Identification procedure when yeast cells are found in brain abscess or CSF specimen. See Fig. 8.3.1–1 for common yeast agents isolated from different body sites. (B) Identification procedure for yeasts when the specimen is not brain abscess or CSF.

Citation: Leber A. 2016. Examination and Evaluation of Primary Cultures, p 8.5.1-8.5.10. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.5
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Image of Figure 8.5–1a
Figure 8.5–1a

Flowchart for evaluating a possible yeast species on primary culture. (A) Identification procedure when yeast cells are found in brain abscess or CSF specimen. See Fig. 8.3.1–1 for common yeast agents isolated from different body sites. (B) Identification procedure for yeasts when the specimen is not brain abscess or CSF.

Citation: Leber A. 2016. Examination and Evaluation of Primary Cultures, p 8.5.1-8.5.10. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.5
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Image of Figure 8.5–2
Figure 8.5–2

Simple tests to determine genera of yeast cells seen on primary culture. (A and B) Identification procedures when colony color is white, cream, or tan. (C) Identification procedure when colony color is salmon, pink, or red. (D) Identification procedure when colony color is brown or black.

Citation: Leber A. 2016. Examination and Evaluation of Primary Cultures, p 8.5.1-8.5.10. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.5
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Download as Powerpoint

References

/content/book/10.1128/9781555818814.chap8.5
1. Odds FC, R. Bernaerts 1994. CHROMagar Candida a new differential isolation medium for presumptive identification of clinically important Candida species. J Clin Microbiol 32:19231929.
2. A-M Freydiere, Guinet R, P. Boiron 2001. Yeast identification in the clinical laboratory: phenotypic methods. Med Mycol 39:933.
3. L. Haley 1978. Laboratory Methods in Medical Mycology. U.S. Department of Health, Education, and Welfare, CDC publication no. 78-8361, p 94.U.S. Department of Health, Education, and Welfare, Washington, DC.
4. Haley LD, CS. Callaway 1978. Laboratory Methods in Medical Mycology. U.S. Department of Health, Education, and Welfare, CDC publication no. 78-8361, p 30. U.S. Department of Health, Education, and Welfare, Washington, DC.

Tables

Generic image for table
Table 8.5–1

General list of yeast species or genera based on colony color

Citation: Leber A. 2016. Examination and Evaluation of Primary Cultures, p 8.5.1-8.5.10. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.5
Generic image for table
Table 8.5–2

Summary of subsequent tests

Citation: Leber A. 2016. Examination and Evaluation of Primary Cultures, p 8.5.1-8.5.10. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.5

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