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Chapter 8.6 : Presumptive Identification Tests for Yeasts Isolated on Primary Culture

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Presumptive Identification Tests for Yeasts Isolated on Primary Culture, Page 1 of 2

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Abstract:

All of the tests described in this procedure ( Table 8.6–1 ) are considered presumptive because they do not test a characteristic of a species that is unique to that species. Some of the tests do have high specificity values, which would make the test sufficient for the purpose of medical management of some clinical situations (e.g., intertriginous candidiasis due to ) but insufficient for others (e.g., fungemia due to ). Presumptive tests also are generally restricted in the range of the species they identify. The results of two different physiological tests with high specificity for a particular species may be appropriate for identifying the species presumptively. However, there are instances when two different species elicit identical positive reactions in both tests. Mycologists and clinical microbiologists must be aware of these obfuscations. For example, by far the most frequently encountered sp. in the clinical setting, and are both germ tube positive and positive for the enzymes β-galactosaminidase and -proline aminopeptidase ( ).

Citation: Leber A. 2016. Presumptive Identification Tests for Yeasts Isolated on Primary Culture, p 8.6.1-8.6.10. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.6
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Figures

Image of Figure 8.6–1
Figure 8.6–1

Algorithm for identification of on primary culture. Follow specimen guidelines for significance for specimens submitted for routine bacteriology cultures. When a protocol requires screening tests or identification for yeasts, proceed as outlined in this diagram. If the yeast is from a sterile body fluid or blood, two tests must be positive to identify or (e.g., if the screen was positive, perform a germ tube test). Note that is a rare to occasional bloodstream pathogen (usually associated with catheters) depending on its endemic density. To discriminate between and use growth at 45°C (presumptive, as not all isolates of tolerate this temperature and not all isolates of are inhibited by it) or, more definitively, assimilation tests ( procedure 8.8). If yeasts are seen in a blood culture bottle, subculture the bottles to CHROMagar to check for purity. SGA, Sabouraud glucose agar; QNS, quantity not sufficient.

Citation: Leber A. 2016. Presumptive Identification Tests for Yeasts Isolated on Primary Culture, p 8.6.1-8.6.10. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.6
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Image of Figure 8.6–2
Figure 8.6–2

Identification of the most common clinically important species isolated from sterile body specimens. Algorithm assumes that CHROMagar was included in the primary set up media. Y, yes; N, no.

Citation: Leber A. 2016. Presumptive Identification Tests for Yeasts Isolated on Primary Culture, p 8.6.1-8.6.10. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.6
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References

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1. Spicer AD, KC. Hazen 1992. Rapid confirmation of Candida albicans identification by combination of two presumptive tests. Med Microbiol Lett 1:284289.
2. A-M Freydiere, Guinet R, P. Boiron 2001. Yeast identification in the clinical microbiology laboratory: phenotypical methods. Med Mycol 39:933.
3. Fenn JP, Billetdeaux E, Segal H, Skodack-Jones L, Padilla PE, Bale M, K. Carroll 1999. Comparison of four methodologies for rapid and cost-effective identification of Candida glabrata. J Clin Microbiol 37:33873389.
4. Carr-Scarborough Microbiologicals, Inc. 1998. Package insert no. 1423-1290. Carr-Scarborough Microbiologicals, Inc., Decatur, GA (C. albicans screen).
5. Hopfer RL, D. Gröschel 1975. Six-hour pigmentation test for the identification of Cryptococcus neoformans. J Clin Microbiol 2:9698.
6. Remel. 1997. Package insert. Rapid trehalose assimilation broth, TI no. 64856. Remel, Lenexa, KS.
7. Remel. 1998. Technical information bulletin TI no. 21128. Remel, Lenexa, KS (Caffeic acid screen).
1. Hopkins JM, GA. Land 1977. Rapid method for determining nitrate utilization by yeasts. J Clin Microbiol 5:497500.

Tables

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Table 8.6–1

Presumptive identification tests for yeasts on primary culture

Citation: Leber A. 2016. Presumptive Identification Tests for Yeasts Isolated on Primary Culture, p 8.6.1-8.6.10. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.6
Generic image for table
Untitled

Citation: Leber A. 2016. Presumptive Identification Tests for Yeasts Isolated on Primary Culture, p 8.6.1-8.6.10. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.6

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