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Chapter 8.9 : Mould Identification

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Mould Identification, Page 1 of 2

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Abstract:

“Mould” is an informal term signifying a fungus growing mostly or entirely in the form of diffuse filaments and usually producing an asexual reproductive state or a sexual state that is not a large, complex fruiting body. Most such fungi are recognized as asexual states or asexual species (the former have known sexual states, while the latter do not) related to various fungi in the phyla Ascomycota and Basidiomycota. In addition, members of the phylum Glomeromycota generally grow as moulds.

Citation: Leber A. 2016. Mould Identification, p 8.9.1-8.9.59. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.9
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Figures

Image of Figure 8.9–A1
Figure 8.9–A1

Sporangiophores and sporangia of (schematic).

Citation: Leber A. 2016. Mould Identification, p 8.9.1-8.9.59. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.9
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Image of Figure 8.9–A2
Figure 8.9–A2

Sporangiophores and rhizoids of (schematic).

Citation: Leber A. 2016. Mould Identification, p 8.9.1-8.9.59. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.9
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Image of Figure 8.9–A3
Figure 8.9–A3

Simple arthroconidia of

Citation: Leber A. 2016. Mould Identification, p 8.9.1-8.9.59. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.9
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Image of Figure 8.9–A4
Figure 8.9–A4

Alternate arthroconidia of (schematic).

Citation: Leber A. 2016. Mould Identification, p 8.9.1-8.9.59. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.9
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Image of Figure 8.9–A5
Figure 8.9–A5

Macroconidium (left) and microconidia (right) of (schematic).

Citation: Leber A. 2016. Mould Identification, p 8.9.1-8.9.59. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.9
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Image of Figure 8.9–A6
Figure 8.9–A6

Blastoconidia of (schematic).

Citation: Leber A. 2016. Mould Identification, p 8.9.1-8.9.59. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.9
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Image of Figure 8.9–A7
Figure 8.9–A7

Sympodially proliferating conidiogenous cell with blastoconidia of in rosette (schematic).

Citation: Leber A. 2016. Mould Identification, p 8.9.1-8.9.59. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.9
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Image of Figure 8.9–A8
Figure 8.9–A8

Phialide of (schematic).

Citation: Leber A. 2016. Mould Identification, p 8.9.1-8.9.59. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.9
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Image of Figure 8.9–A9
Figure 8.9–A9

structures.

Citation: Leber A. 2016. Mould Identification, p 8.9.1-8.9.59. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.9
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Image of Figure 8.9–A10
Figure 8.9–A10

Annellides of (schematic).

Citation: Leber A. 2016. Mould Identification, p 8.9.1-8.9.59. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.9
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Image of Figure 8.9–A11
Figure 8.9–A11

Pycnidia of face view and cross-section (schematic).

Citation: Leber A. 2016. Mould Identification, p 8.9.1-8.9.59. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.9
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Image of Figure 8.9–A12
Figure 8.9–A12

Ascoma structures of (one of several sexual states of spp.) (schematic) as seen at different magnifications (cleistothecium with asci, as seen at ×100; ascus with ascospores, as seen at ×400; released ascospores, as seen at ×1,000).

Citation: Leber A. 2016. Mould Identification, p 8.9.1-8.9.59. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.9
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Image of Figure 8.9–A13
Figure 8.9–A13

Cell division in a dikaryotic basidiomycetous hypha, showing development of a clamp connection.

Citation: Leber A. 2016. Mould Identification, p 8.9.1-8.9.59. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.9
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Image of Figure 8.9–A14
Figure 8.9–A14

Basidium and basidiospores.

Citation: Leber A. 2016. Mould Identification, p 8.9.1-8.9.59. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.9
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Image of Figure 8.9–A15
Figure 8.9–A15

Terminal chlamydospore.

Citation: Leber A. 2016. Mould Identification, p 8.9.1-8.9.59. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.9
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Image of Figure 8.9–A16
Figure 8.9–A16

Chain of symmetrical chlamydospores.

Citation: Leber A. 2016. Mould Identification, p 8.9.1-8.9.59. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.9
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Image of Figure 8.9–A17
Figure 8.9–A17

Chain of asymmetrical chlamydospores (cow’s teeth).

Citation: Leber A. 2016. Mould Identification, p 8.9.1-8.9.59. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.9
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Image of Figure 8.9–A18
Figure 8.9–A18

Pedicels (matchstick stalks) of (schematic).

Citation: Leber A. 2016. Mould Identification, p 8.9.1-8.9.59. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.9
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Image of Figure 8.9–A19
Figure 8.9–A19

Macroconidia of (schematic) with cytoplasmic compartments indicated by dotted lines.

Citation: Leber A. 2016. Mould Identification, p 8.9.1-8.9.59. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.9
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Image of Figure 8.9–A20
Figure 8.9–A20

Helical seta (spiral appendage) of (schematic).

Citation: Leber A. 2016. Mould Identification, p 8.9.1-8.9.59. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.9
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Image of Figure 8.9–A21
Figure 8.9–A21

Reflexive branching of (schematic).

Citation: Leber A. 2016. Mould Identification, p 8.9.1-8.9.59. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.9
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Image of Figure 8.9–A22
Figure 8.9–A22

Swollen nailhead hyphae of (schematic).

Citation: Leber A. 2016. Mould Identification, p 8.9.1-8.9.59. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.9
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Image of Figure 8.9–A23
Figure 8.9–A23

Hypha with pectinate branching as seen in (schematic).

Citation: Leber A. 2016. Mould Identification, p 8.9.1-8.9.59. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.9
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Image of Figure 8.9–A24
Figure 8.9–A24

Lateral hyphal “projection” or diverticulum of (schematic).

Citation: Leber A. 2016. Mould Identification, p 8.9.1-8.9.59. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.9
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References

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1. Aly R. 1994. Culture media for growing dermatophytes. J Am Acad Dermatol 31:S107S108.
2. Sigler L. 1997. Chrysosporium and molds resembling dermatophytes, p 261311. In Kane J, Summerbell RC, Sigler L, Krajden S, Land G (ed), Laboratory Handbook of Dermatophytes. Star Publishing, Belmont, CA.
3. Kane J, Summerbell RC, Sigler L, Krajden S, Land G. 1997. Laboratory Handbook of Dermatophytes, p 324. Star Publishing, Belmont, CA.
4. Summerbell RC, Kane J. 1997. Physiological and other special tests for identifying dermatophytes, p 4577. In Kane J, Summerbell RC, Sigler L, Krajden S, Land G (ed), Laboratory Handbook of Dermatophytes. Star Publishing, Belmont, CA.
5. Kaminsky GW. 1985. The routine use of modified Borelli’s lactrimel agar (MBLA). Mycopathologia 91:5759.

Tables

Generic image for table
Table 8.9–1

Definitions of terms used to describe morphological structures

Citation: Leber A. 2016. Mould Identification, p 8.9.1-8.9.59. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.9
Generic image for table
Table 8.9–2

Phenotypic characteristics useful for identification of Zygomycota

Citation: Leber A. 2016. Mould Identification, p 8.9.1-8.9.59. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.9
Generic image for table
Table 8.9–3

Description of terms for conidial ontogeny and reproductive structures of the Ascomycota

Citation: Leber A. 2016. Mould Identification, p 8.9.1-8.9.59. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.9
Generic image for table
Table 8.9–4

Common media used for moulds

Citation: Leber A. 2016. Mould Identification, p 8.9.1-8.9.59. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.9
Generic image for table
Table 8.9–5

Temperature tests commonly used in identification of clinically important moulds

Citation: Leber A. 2016. Mould Identification, p 8.9.1-8.9.59. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.9
Generic image for table
Table 8.9–6

Cycloheximide responses of common or critical clinical laboratory moulds (including abundant contaminants)

Citation: Leber A. 2016. Mould Identification, p 8.9.1-8.9.59. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.9
Generic image for table
Table 8.9–8

Particulate phases of dimorphic fungi as seen in conversion and their differentiating characteristics

Citation: Leber A. 2016. Mould Identification, p 8.9.1-8.9.59. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.9
Generic image for table
Table 8.9–7

Distinguishing features for preliminary identification of mould phases of dimorphic systemic pathogens

Citation: Leber A. 2016. Mould Identification, p 8.9.1-8.9.59. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.9
Generic image for table
Table 8.9–9

Dermatophyte micromorphological structures

Citation: Leber A. 2016. Mould Identification, p 8.9.1-8.9.59. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.9
Generic image for table
Table 8.9–10

Phenotypic identification characteristics of common and occasionally seen dermatophytes, as well as rare species potentially used in QC trials

Citation: Leber A. 2016. Mould Identification, p 8.9.1-8.9.59. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.9
Generic image for table
Table 8.9–11

Most common fungi other than dermatophytes and repeatedly and rigorously implicated as causal agents of onychomycosis

Citation: Leber A. 2016. Mould Identification, p 8.9.1-8.9.59. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch8.9

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