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Chapter 9.4 : S S C M

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S S C M, Page 1 of 2

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Abstract:

and () species have been recognized as causes of severe diarrhea in immunocompromised hosts, but they can also cause diarrhea in immunocompetent hosts. Oocysts in clinical specimens may be difficult to detect without special staining. has also been reported to be acid fast. Modified acid-fast stains are recommended for demonstrating these organisms. Unlike the Ziehl-Neelsen modified acid-fast stain variable, the modified Kinyoun acid-fast stain does not require heating the reagents used for staining and uses a mild decolorizer ( ).

Citation: Leber A. 2016. S S C M, p 9.4.1.1-9.4.7.5. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch9.4
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Figures

Image of Figure 9.4.1–A1
Figure 9.4.1–A1

(Top) oocysts; sporozoites are visible within some oocysts. (Bottom) () immature oocyst; note that the entire oocyst stains with modified acid-fast stain.

Citation: Leber A. 2016. S S C M, p 9.4.1.1-9.4.7.5. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch9.4
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Image of Figure 9.4.2–A1
Figure 9.4.2–A1

(Left) oocysts (note variation in staining). (Right) The large, clear object is a oocyst (8 to 10 μm); the darker object to the right is a oocyst (4 to 6 μm). Note that tends to look like wrinkled cellophane when it does not stain well.

Citation: Leber A. 2016. S S C M, p 9.4.1.1-9.4.7.5. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch9.4
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References

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