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Chapter 9.5 : A T S E

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A T S E, Page 1 of 2

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Abstract:

larvae are usually the only larvae found in stool specimens. Depending on bowel transit time and the condition of the patient, rhabditiform and, rarely, filariform larvae may be present. If there is delay in examination of the stool, then embryonated ova and larvae of hookworm may be present. Culture of feces for larvae is useful to (i) reveal the presence of larvae when they are too scanty to be detected by concentration methods, (ii) distinguish whether the infection is due to or hook-worm on the basis of rhabditiform larval morphology by allowing hookworm eggs to hatch and release first-stage larvae, and (iii) allow development of larvae into the filariform stage for further differentiation.

Citation: Leber A. 2016. A T S E, p 9.5.1.1-9.5.8.2. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch9.5
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Figures

Image of Figure 9.5.1–1
Figure 9.5.1–1

Diagram of the Baermann apparatus used for recovery of larval-stage nematodes (from reference ).

Citation: Leber A. 2016. A T S E, p 9.5.1.1-9.5.8.2. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch9.5
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Image of Figure 9.5.2–1
Figure 9.5.2–1

Diagram of Harada-Mori culture system (from reference ).

Citation: Leber A. 2016. A T S E, p 9.5.1.1-9.5.8.2. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch9.5
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Image of Figure 9.5.3–1
Figure 9.5.3–1

Diagram of petri dish-filter paper slant (from reference ).

Citation: Leber A. 2016. A T S E, p 9.5.1.1-9.5.8.2. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch9.5
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Image of Figure 9.5.4–1
Figure 9.5.4–1

Agar culture method for (1) Agar plates are prepared; (2) agar is dried for 4 to 5 days on the bench top; (3) plates are stored in plastic bags; (4) fresh stool is submitted to the laboratory; (5) approximately 2 g of stool is placed onto an agar plate; (6) the plate is sealed with tape; (7) the culture plate is incubated at 26 to 33°C for 2 days; (8) the plate is examined microscopically for the presence of tracks (bacteria carried over agar by migrating larvae); (9) 10% formalin is placed onto agar through a hole made in the plastic with hot forceps; and (10) material from the agar plate is centrifuged and (11) examined as a wet preparation for rhabditiform or filariform larvae (high dry power; magnification, ×400). (Illustration by Sharon Belkin; from reference ).

Citation: Leber A. 2016. A T S E, p 9.5.1.1-9.5.8.2. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch9.5
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Image of Figure 9.5.4–A1
Figure 9.5.4–A1

Tracking of larvae on agar plate culture.

Citation: Leber A. 2016. A T S E, p 9.5.1.1-9.5.8.2. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch9.5
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Image of Figure 9.5.5–1
Figure 9.5.5–1

Diagram of a sidearm hatching flask (from reference ).

Citation: Leber A. 2016. A T S E, p 9.5.1.1-9.5.8.2. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch9.5
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Image of Figure 9.5.5–2
Figure 9.5.5–2

Diagram of a schistosomal miradicium within the egg shell (from reference ).

Citation: Leber A. 2016. A T S E, p 9.5.1.1-9.5.8.2. In Clinical Microbiology Procedures Handbook, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818814.ch9.5
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References

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1. Baermann G. 1917. Eine einfache Methode zur Auffindung vor Ankylostomum (Nematoden) Larven in Erdproben. Meded Geneesk Laborat Weltever Feestbundel, p 41.
2. Garcia LS. 2016. Diagnostic Medical Parasitology, 6th ed. ASM Press, Washington, DC.
3. Melvin DM, Brooke MM. 1985. Laboratory Procedures for the Diagnosis of Intestinal Parasites, p 163189. U.S. Department of Health, Education, and Welfare publication no. (CDC) 85-8282. U.S. Government Printing Office, Washington, DC.
4. Shorb DA. 1937. A method of separating infective larvae of Haemonchus contortus (Trichostrongylidae) from free living nematodes. Proc Helminthol Soc Wash 4:52.
5. Watson JM, Al-Hafidh R. 1957. A modification of the Baermann funnel technique and its use in establishing the infection potential of human hookworm carriers. Ann Trop Med Parasitol 51:1516.
6. Ash LR, Origel TC. 1987. Parasites: a Guide to Laboratory Procedures and Identification. ASCP Press, Chicago, IL.
7. Markell EK, John DT, Krotoski WA. 1999. Markell and Voge’s Medical Parasitology, 8th ed. W. B. Saunders Co., Philadelphia, PA.
1. Garcia LS. 2016. Diagnostic Medical Parasitology, 6th ed. ASM Press, Washington, DC.
2. Harada U, Mori O. 1955. A new method for culturing hookworm. Yonago Acta Med. 1:177179.
3. Hsieh HC. 1962. A test-tube filter-paper method for the diagnosis of Ancylostoma duodenale, Necator americanus, and Strongyloides stercoralis. WHO Tech Rep Ser 255:2730.
4. Melvin DM, Brooke MM. 1985. Laboratory Procedures for the Diagnosis of Intestinal Parasites, p 163189. U.S. Department of Health, Education and Welfare publication no. (CDC) 85-8282. U.S. Government Printing Office, Washington, DC.
5. Sasa M, Hayashi S, Tanaka H, Shirasaka R. 1958. Application of test-tube cultivation method on the survey of hookworm and related human nematode infection. Jpn J Exp Med 28:129137.
6. Shorb DA. 1937. A method of separating infective larvae of Haemonchus contortus (Trichostrongylidae) from free living nematodes. Proc Helminthol Soc Wash 4:52.
14. Ash LR, Orihel TC. 1987. Parasites: a Guide to Laboratory Procedures and Identification. ASCP Press, Chicago, IL.
15. Markell EK, John DT, Krotoski WA. 1999. Markell and Voge’s Medical Parasitology, 8th ed. W. B. Saunders Co., Philadelphia, PA.
1. Garcia LS. 2016. Diagnostic Medical Parasitology, 6th ed. ASM Press, Washington, DC.
2. Little MD. 1966. Comparative morphology of six species of Strongyloides (Nematoda) and redefinition of the genus. J Parasitol 52:6984.
3. Melvin DM, Brooke MM. 1985. Laboratory Procedures for the Diagnosis of Intestinal Parasites, p 163189. U.S. Department of Health, Education, and Welfare publication no. (CDC) 85-8282. U.S. Government Printing Office, Washington, DC.
4. Shorb DA. 1937. A method of separating infective larvae of Haemonchus contortus (Trichostrongylidae) from free living nematodes. Proc Helm Soc Wash 4:52.
20. Ash LR, Orihel TC. 1987. A Guide to Laboratory Procedures and Identification. ASCP Press, Chicago, IL.
21. Markell EK, John DT, Krotoski WA. 1999. Markell and Voge’s Medical Parasitology, 8th ed. W. B. Saunders Co., Philadelphia, PA.
1. Arakaki T, Iwanaga M, Kinjo F, Saito A, Asato R, Ikeshiro T. 1990. Efficacy of agar-plate culture in detection of Strongyloides stercoralis infection. J Parasitol 76:425428.
2. Garcia LS. 2016. Diagnostic Medical Parasitology, 6th ed. ASM Press, Washington, DC.
3. Garcia LS. 2009. Practical Guide to Diagnostic Parasitology, 2nd ed. ASM Press, Washington, DC.
4. Koga KS, Kasuya C, Khamboonruang K, Sukhavat M, Ieda M, Takatsuka N, Kita K, Ohtomo H. 1991. A modified agar plate method for detection of Strongyloides stercoralis. Am J Trop Med Hyg 45:518521.
5. Kobayashi J, Hasegawa H, Soares EC, Toma H, Dacal AR, Brito MC, Yamanaka A, Foli AA, Sato Y. 1996. Studies on prevalence of Strongyloides infection in Holambra and Maceio, Brazil, by the agar plate faecal culture method. Rev Inst Med Trop Sao Paulo 38:279284.
6. Uparanukraw P, Phongsri S, Morakote N. 1999. Fluctuations of larval excretion in Strongyloides stercoralis infection. Am J Trop Med Hyg 60:967973.
7. Iwamoto T, Kitoh M, Kayashima K, Ono T. 1998. Larva currens: the usefulness of the agar plate method. Dermatology 196:343345.
8. Jongwutiwes S, Charoenkorn M, Sitthichareonchai P, Akaraborvorn P, Putaporntip C. 1999. Increased sensitivity of routine laboratory detection of Strongyloides stercoralis and hookworm by agar-plate culture. Trans R Soc Trop Med Hyg 93:398400.
9. Corsetti M, Basilisco G, Pometta R, Allocca M, Conte D. 1999. Mistaken diagnosis of eosinophilic colitis. Ital J Gastroenterol Hepatol 31:607609.
10. Gotuzzo E, Arango C, de Queiroz-Campos A, Isturiz RE. 2000. Human T-cell lympho-tropic virus-I in Latin America. Infect Dis Clin N Am 14:211239, xxi.
1. Chernin E, Dunavan CA. 1962. The influence of host-parasite dispersion upon the capacity of Schistosoma mansoni miracidia to infect Australorbis glabratus. Am J Trop Med Hyg 11:455471.
2. Garcia LS. 2016. Diagnostic Medical Parasitology, 6th ed. ASM Press, Washington, DC.
3. McMullen DB, Beaver PC. 1945. Studies on schistosome dermatitis. IX. The life cycles of three dermatitis producing schistosomes from birds and a discussion of the subfamily Bilharziellinae (Trematodai Schistosomatidae). Am J Hyg 42:125154.
4. Melvin DM, Brooke MM. 1985. Laboratory Procedures for the Diagnosis of Intestinal Parasites, p 179181. US Department of Health, Education and Welfare publication no (CDC) 85-9393. US Government Printing Office, Washington, DC.
1. Garcia L. S. 2016. Diagnostic Medical Parasitology, 6th ed. ASM Press, Washington, DC.
2. Melvin DM, Brooke MM. 1985. Laboratory Procedures for the Diagnosis of Intestinal Parasites, p 187. US Department of Health, Education and Welfare publication no (CDC) 85-8282. US Government Printing Office, Washington, DC.
38. Drummey BS, Benson JAJr, Jones CM. 1961. Microscopic examination of the stool for steatorrhea. N Engl J Med 264:8587.
39. Henry JB. 2001. Clinical Diagnosis and Management by Laboratory Methods, 20th ed. WB Saunders Company, Philadelphia, PA.
40. Bayer Corporation Diagnostic Division. September 2010. Clinitest package insert. Bayer Corporation Diagnostic Division, Elkhart, IN.
41. Davidson G, Mullinger M. 1970. Reducing substances in neonatal stool detected by Clinitest. J Pediatr. 46:632635.
42. Kerry KR, Anderson CM. 1964. A ward test for sugar in feces. Lancet i:981.

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