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Chapter 3.2.9 : and Legionnaires’ Disease

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and Legionnaires’ Disease, Page 1 of 2

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Abstract:

This chapter details the ecology of Legionnaires' disease and a description of its history in regards to public health. Also included are methods for detection in environmental samples, identification and environmental approaches to controlling legionellosis.

Citation: Lucas C, Fields B. 2016. and Legionnaires’ Disease, p 3.2.9-1-3.2.9-13. In Yates M, Nakatsu C, Miller R, Pillai S (ed), Manual of Environmental Microbiology, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818821.ch3.2.9
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Figures

Image of FIGURE 1
FIGURE 1

Gimenez stain of the ciliated protozoan infected with . Chains of multiplying cells are contained within vesicles, as observed in human phagocytic cells infected with . Magnification, ×1,650. doi:10.1128/9781555818821.ch3.2.9.f1

Citation: Lucas C, Fields B. 2016. and Legionnaires’ Disease, p 3.2.9-1-3.2.9-13. In Yates M, Nakatsu C, Miller R, Pillai S (ed), Manual of Environmental Microbiology, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818821.ch3.2.9
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Image of FIGURE 2
FIGURE 2

Overview of procedures for the culture of water samples to detect . doi:10.1128/9781555818821.ch3.2.9.f2

Citation: Lucas C, Fields B. 2016. and Legionnaires’ Disease, p 3.2.9-1-3.2.9-13. In Yates M, Nakatsu C, Miller R, Pillai S (ed), Manual of Environmental Microbiology, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818821.ch3.2.9
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Image of FIGURE 3
FIGURE 3

Two colonies and a non- bacterial colony as seen through a dissecting microscope upon primary isolation (4 days of incubation). Note the white “cut-glass” appearance of the center of the colony and the purple iridescence which borders it. The iridescence can be one of several colors; the significance of the colors is unknown. doi:10.1128/9781555818821.ch3.2.9.f3

Citation: Lucas C, Fields B. 2016. and Legionnaires’ Disease, p 3.2.9-1-3.2.9-13. In Yates M, Nakatsu C, Miller R, Pillai S (ed), Manual of Environmental Microbiology, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818821.ch3.2.9
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Image of FIGURE 4
FIGURE 4

Algorithm for speciating and subtyping genus-confirmed isolates. There are numerous commercial vendors of reagents to perform DFA or slide agglutination to quickly determine whether an isolate is species and, if so, whether it belongs to serogroup 1 (Lp1) or serogroups 2–14 (Lp2–14). Lp1 isolates may be further subtyped by SBT, serology with a panel of monoclonal antibodies, and/or fragment analyses such as PFGE. Commercial antisera also exist to specifically serotype Lp2–14 isolates. Subtyping Lp2–14 isolates may be achieved by SBT and/or fragment analyses. Speciating nonpneumophila spp. isolates is accomplished by employing species-specific antisera in DFA or slide agglutination tests or by sequencing the gene. Genes for all seven of the genes used in SBT are generally not present in nonpneumophila species, so the only way to reliably subtype these isolates is by fragment analyses. may not display cysteine auxotrophy. When performing comprehensive environmental surveys or where epidemiological evidence suggests the involvement of this species in cases of disease, it may be advantageous to subject suspect colonies to the nonpneumophila spp. branch (far right) of the algorithm.There are no commercially available sources of the international panel for monoclonal antibody subtyping. However, limited supplies may be obtained for research or public health surveillance purposes from the developers ( ). doi:10.1128/9781555818821.ch3.2.9.f4

Citation: Lucas C, Fields B. 2016. and Legionnaires’ Disease, p 3.2.9-1-3.2.9-13. In Yates M, Nakatsu C, Miller R, Pillai S (ed), Manual of Environmental Microbiology, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818821.ch3.2.9
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References

/content/book/10.1128/9781555818821.ch3.2.9
1. Hicks LA, Garrison LE, Nelson GE, Hampton LM. 2012. Legionellosis—United States, 2000–2009. Am J Trans 12:250253.[CrossRef]
2. Brunkard JM, Ailes E, Roberts VA, Hill V, Hilborn ED, Craun GF, Rajasingham A, Kahler A, Garrison L, Hicks L, Carpenter J, Wade TJ, Beach MJ, Yoder JS CDC. 2011. Surveillance for waterborne disease outbreaks associated with drinking water—United States, 2007–2008. Morbid Mortal Weekly Rep Surveill Summ 60:3868.
3. Palusinska-Szysz M, Cendrowska-Pinkosz M. 2009. Pathogenicity of the family Legionellaceae. Arch Immunol Ther Exp 57:279290.[CrossRef]
4. McDade JE, Shepard CC, Fraser DW, Tsai TR, Redus MA, Dowdle WR. 1977. Legionnaires’ disease: isolation of a bacterium and demonstration of its role in other respiratory disease. N Eng J Med 297:11971203.[CrossRef]
5. Fraser DW, Tsai TR, Orenstein W, Parkin WE, Beecham HJ, Sharrar RG, Harris J, Mallison GF, Martin SM, McDade JE, Shepard CC, Brachman PS. 1977. Legionnaires’ disease: description of an epidemic of pneumonia. N Eng J Med 297:11891197.[CrossRef]
6. Fields BS, Benson RF, Besser RE. 2002. Legionella and Legionnaires’ disease: 25 years of investigation. Clin Microbiol Rev 15:506526.[PubMed][CrossRef]
7. Molmeret M, Bitar DM, Han L, Kwaik YA. 2004. Cell biology of the intracellular infection by Legionella pneumophila. Micr Infect 6:129139.[CrossRef]
8. Friedman H, Yamamoto Y, Klein TW. 2002. Legionella pneumophila pathogenesis and immunity. Sem Ped Infect Dis 13:273279.[CrossRef]
9. Castor ML, Wagstrom EA, Danila RN, Smith KE, Naimi TS, Besser JM, Peacock KA, Juni BA, Hunt JM, Bartkus JM, Kirkhorn SR, Lynfield R. 2005. An outbreak of Pontiac fever with respiratory distress among workers performing high-pressure cleaning at a sugar-beet processing plant. J Infect Dis 191:15301537.[PubMed][CrossRef]
10. Fields BS, Haupt T, Davis JP, Arduino MJ, Miller PH, Butler JC. 2001. Pontiac fever due to Legionella micdadei from a whirlpool spa: possible role of bacterial endotoxin. J Infect Dis 184:12891292.[PubMed][CrossRef]
11. Hadler SC, Castro KG, Dowdle W, Hicks L, Noble G, Ridzon R. 2011. Epidemic Intelligence Service investigations of respiratory illness, 1946–2005. Am J Epidemiol 174:S36S46.[PubMed][CrossRef]
12. Hollenbeck B, Dupont I, Mermel LA. 2011. How often is a work-up for Legionella pursued in patients with pneumonia? a retrospective study. BMC Infect Dis 11:237.[PubMed][CrossRef]
13. Tronel H, Hartemann P. 2009. Overview of diagnostic and detection methods for legionellosis and Legionella spp. Lett Appl Microbiol 48:653656.[PubMed]
14. Lim YH, Relus Kek YL, Lim PY, Yap HM, Vivien Goh TL, Ng LC. 2011. Environmental surveillance and molecular characterization of Legionella in tropical Singapore. Trop Biomed 28:149159.[PubMed]
15. Lee HK, Shim JI, Kim HE, Yu JY, Kang YH. 2010. Distribution of Legionella species from environmental water sources of public facilities and genetic diversity of L. pneumophila serogroup 1 in South Korea. Appl Environ Microbiol 76:65476554.[PubMed][CrossRef]
16. Kozak NA, Benson RF, Brown E, Alexander NT, Taylor THJr., Shelton BG, Fields BS. 2009. Distribution of lag-1 alleles and sequence-based types among Legionella pneumophila serogroup 1 clinical and environmental isolates in the United States. J Clin Microbiol 47:25252535.[PubMed][CrossRef]
17. Den Boer JW, Verhoef L, Bencini MA, Bruin JP, Jansen R, Yzerman EP. 2007. Outbreak detection and secondary prevention of Legionnaires’ disease: a national approach. Int J Hyg Envir Heal 210:17.[CrossRef]
18. Sehulster L, Chinn RY CDC, HICPAC. 2003. Guidelines for environmental infection control in health-care facilities. Recommendations of CDC and the Healthcare Infection Control Practices Advisory Committee (HICPAC). MMWR Recomm Rep 52:142.[PubMed]
19. Tablan OC, Anderson LJ, Besser R, Bridges C, Hajjeh R CDC, HICPAC. 2004. Guidelines for preventing health-care–associated pneumonia, 2003: recommendations of CDC and the Healthcare Infection Control Practices Advisory Committee. MMWR Recomm Rep 53:136.[PubMed]
20. Brenner DJ, Steigerwalt AG, McDade JE. 1979. Classification of the Legionnaires’ disease bacterium: Legionella pneumophila, genus novum, species nova, of the family Legionellaceae, familia nova. Ann Int Med 90:656658.[PubMed][CrossRef]
21. Edelstein PH, Edelstein MA, Shephard LJ, Ward KW, Ratcliff RM. 2012. Legionella steelei sp. nov., isolated from human respiratory specimens in California, USA, and South Australia. Int. J. Syst. Evol. Micr 62:17661771.[CrossRef]
22. Graham FF, White PS, Harte DJ, Kingham SP. 2012. Changing epidemiological trends of legionellosis in New Zealand, 1979–2009. Epidemiol Infect 140:14811496.[PubMed][CrossRef]
23. Alli OA, Zink S, Von Lackum NK, Abu-Kwaik Y. 2003. Comparative assessment of virulence traits in Legionella spp. Microbiology 149(Pt 3):631641.[PubMed][CrossRef]
24. Lamoth F, Greub G. 2010. Amoebal pathogens as emerging causal agents of pneumonia. FEMS Microbiol Rev 34:260280.[PubMed][CrossRef]
25. Carvalho FR, Nastasi FR, Gamba RC, Foronda AS, Pellizari VH. 2008. Occurrence and diversity of Legionellaceae in polar lakes of the Antarctic peninsula. Curr Microbiol 57:294300.[PubMed][CrossRef]
26. Ott CM, Bruce RJ, Pierson DL. 2004. Microbial characterization of free floating condensate aboard the Mir space station. Microbial Ecol 47:133136.[CrossRef]
27. Katz SM, Hammel JM. 1987. The effect of drying, heat, and pH on the survival of Legionella pneumophila. Ann Clin Lab Sci 17:150156.[PubMed]
28. Mermel LA, Josephson SL, Giorgio CH, Dempsey J, Parenteau S. 1995. Association of Legionnaires’ disease with construction: contamination of potable water? Infect Control Hosp Epidemiol 16:7681.[PubMed][CrossRef]
29. Whiley H, Bentham R. 2011. Legionella longbeachae and legionellosis. Emerg Infect Dis 17:579583.[PubMed][CrossRef]
30. Silk BJ, Moore MR, Bergtholdt M, Gorwitz RJ, Kozak NA, Tha MM, Brown EW, Winchester JL, Labus BJ, Rowley P, Middaugh JP, Fields BS, Hicks LA. 2012. Eight years of Legionnaires’ disease transmission in travellers to a condominium complex in Las Vegas, Nevada. Epidemiol Infect 140:19932002.[PubMed][CrossRef]
31. Rangel-Frausto MS, Rhomberg P, Hollis RJ, Pfaller MA, Wenzel RP, Helms CM, Herwaldt LA. 1999. Persistence of Legionella pneumophila in a hospital's water system: a 13-year survey. Infect Control Hosp Epidemiol 20:793797.[PubMed][CrossRef]
32. Cowgill KD, Lucas CE, Benson RF, Chamany S, Brown EW, Fields BS, Feikin DR. 2005. Recurrence of Legionnaire's disease at a hotel in the United States Virgin Islands over a 20-year period. Clin Infect Dis 40:12051207.[PubMed][CrossRef]
33. Feeley JC, Gibson RJ, Gorman GW, Langford NC, Rasheed JK, Mackel DC, Baine WB. 1979. Charcoal-yeast extract agar: primary isolation medium for Legionella pneumophila. J Clini Microbiol 10:437441.
34. Rowbotham TJ. 1980. Preliminary report on the pathogenicity of Legionella pneumophila for freshwater and soil amoebae. J Clin Pathol 33:11791183.[PubMed][CrossRef]
35. Fields BS. 1996. The molecular ecology of legionellae. Trends Microbiol 4:286290.[PubMed][CrossRef]
36. Solomon JM, Isberg RR. 2000. Growth of Legionella pneumophila in Dictyostelium discoideum: a novel system for genetic analysis of host-pathogen interactions. Trends Microbiol 8:478480.[PubMed][CrossRef]
37. Ensminger AW, Yassin Y, Miron A, Isberg RR. 2012. Experimental evolution of Legionella pneumophila in mouse macrophages leads to strains with altered determinants of environmental survival. PLoS Pathogens 8:e1002731.[PubMed][CrossRef]
38. O'Connor TJ, Adepoju Y, Boyd D, Isberg RR. 2011. Minimization of the Legionella pneumophila genome reveals chromosomal regions involved in host range expansion. Proc Natl Acad Sci USA 108:1473314740.[PubMed][CrossRef]
39. Swanson MS, Hammer BK. 2000. Legionella pneumophila pathogesesis: a fateful journey from amoebae to macrophages. Ann Rev Microbiol 54:567613.[CrossRef]
40. Nagai H, Kubori T. 2011. Type IVB secretion systems of Legionella and other Gram-negative bacteria. Front Microbiol 2:136.[PubMed][CrossRef]
41. Gomez-Valero L, Rusniok C, Cazalet C, Buchrieser C. 2011. Comparative and functional genomics of Legionella identified eukaryotic like proteins as key players in host-pathogen interactions. Front Microbiol 2:208.[PubMed][CrossRef]
42. Rolando M, Buchrieser C. 2012. Post-translational modifications of host proteins by Legionella pneumophila: a sophisticated survival strategy. Fut Microbiol 7:369381.[CrossRef]
43. Luo ZQ. 2012. Legionella secreted effectors and innate immune responses. Cell Microbiol 14:1927.[PubMed][CrossRef]
44. Yoder J, Roberts V, Craun GF, Hill V, Hicks LA, Alexander NT, Radke V, Calderon RL, Hlavsa MC, Beach MJ, Roy SL CDC. 2008. Surveillance for waterborne disease and outbreaks associated with drinking water and water not intended for drinking—United States, 2005–2006. Morbid Mortal Weekly Rep Surveill Summ 57:3962.
45. Phares CR, Russell E, Thigpen MC, Service W, Crist MB, Salyers M, Engel J, Benson RF, Fields B, Moore MR. 2007. Legionnaires’ disease among residents of a long-term care facility: the sentinel event in a community outbreak. Am J Infect Cont 35:319323.[CrossRef]
46. O'Loughlin RE, Kightlinger L, Werpy MC, Brown E, Stevens V, Hepper C, Keane T, Benson RF, Fields BS, Moore MR. 2007. Restaurant outbreak of Legionnaires’ disease associated with a decorative fountain: an environmental and case-control study. BMC Infect Dis 7:93.[PubMed][CrossRef]
47. Allen JG, Myatt TA, Macintosh DL, Ludwig JF, Minegishi T, Stewart JH, Connors BF, Grant MP, McCarthy JF. 2012. Assessing risk of health care-acquired Legionnaires’ disease from environmental sampling: the limits of using a strict percent positivity approach. Am J Infect Contr 40:917921.[CrossRef]
48. Ditommaso S, Giacomuzzi M, Gentile M, Moiraghi AR, Zotti CM. 2010. Effective environmental sampling strategies for monitoring Legionella spp contamination in hot water systems. Am J Infect Cont 38:344349.[CrossRef]
49. Flannery B, Gelling LB, Vugia DJ, Weintraub JM, Salerno JJ, Conroy MJ, Stevens VA, Rose CE, Moore MR, Fields BS, Besser RE. 2006. Reducing Legionella colonization in water systems with monochloramine. Emerg Infect Dis 12:588596.[PubMed][CrossRef]
50. Ohno A, Kato N, Sakamoto R, Kimura S, Yamaguchi K. 2008. Temperature-dependent parasitic relationship between Legionella pneumophila and a free-living amoeba (Acanthamoeba castellanii). Appl Environ Microbiol 74:45854588.[PubMed][CrossRef]
51. Luck PC, Igel L, Helbig JH, Kuhlisch E, Jatzwauk L. 2004. Comparison of commercially available media for the recovery of Legionella species. Int J Hyg Envir Health 207:589593.[CrossRef]
52. Leoni E, Legnani PP. 2001. Comparison of selective procedures for isolation and enumeration of Legionella species from hot water systems. J Appl Microbiol 90:2733.[PubMed][CrossRef]
53. McCoy WF, Downes EL, Leonidas LF, Cain MF, Sherman DL, Chen K, Devender S, Neville MJ. 2012. Inaccuracy in Legionella tests of building water systems due to sample holding time. Water Res 46:34973506.[PubMed][CrossRef]
54. Boulanger CA, Edelstein PH. 1995. Precision and accuracy of recovery of Legionella pneumophila from seeded tap water by filtration and centrifugation. Appl Enbiron Microbiol 61:18051809.
55. Lucas CE, Taylor THJr., Fields BS. 2011. Accuracy and precision of Legionella isolation by US laboratories in the ELITE program pilot study. Water Res 45:44284436.[PubMed][CrossRef]
56. Buse HY, Schoen ME, Ashbolt NJ. 2012. Legionellae in engineered systems and use of quantitative microbial risk assessment to predict exposure. Water Res 46:921933.[PubMed][CrossRef]
57. Schoen ME, Ashbolt NJ. 2011. An in-premise model for Legionella exposure during showering events. Water Res 45:58265836.[PubMed][CrossRef]
58. Morris GK, Shelton BG. 1991. Legionella in environmental samples: hazard analysis and suggested remedial actions. Pathcon Technical Bulletin 13.
59. Best M, Yu VL, Stout J, Goetz A, Muder RR, Taylor F. 1983. Legionellaceae in the hospital water-supply. Epidemiological link with disease and evaluation of a method for control of nosocomial legionnaires’ disease and Pittsburgh pneumonia. Lancet 2:307310.[PubMed][CrossRef]
60. Borella P, Guerrieri E, Marchesi I, Bondi M, Messi P. 2005. Water ecology of Legionella and protozoan: environmental and public health perspectives. Biotechnol Annu Rev 11:35580.[PubMed][CrossRef]
61. Fields BS. 2005. Procedures for the recovery of Legionella from the environment, pp. 113. In: Services HaH, (ed), 2nd ed. Public Health Service, Atlanta, GA.
62. ISO. 1998. Water quality—detection and enumeration of Legionella. ISO, Geneva, Switzerland,.
63. ISO. 2004. Water quality—detection and enumeration of Legionella—Part 2: Direct membrane filtration method for waters with low bacteria counts. ISO, Geneva, Switzerland.
64. Kozak NA, Lucas CE, Winchell JM. 2013. Identification of Legionella in the environment. Meth Molecular Biology 954:325.[CrossRef]
65. Ishimatsu S, Miyamoto H, Hori H, Tanaka I, Yoshida S. 2001. Sampling and detection of Legionella pneumophila aerosols generated from an industrial cooling tower. Ann Occup Hyg 45:421427.[PubMed][CrossRef]
66. Breiman RF, Cozen W, Fields BS, Mastro TD, Carr SJ, Spika JS, Mascola L. 1990. Role of air sampling in investigation of an outbreak of legionnaires’ disease associated with exposure to aerosols from an evaporative condenser. J Infect Dis 161:12571261.[PubMed][CrossRef]
67. Fry AM, Rutman M, Allan T, Scaife H, Salehi E, Benson R, Fields B, Nowicki S, Parrish MK, Carpenter J, Brown E, Lucas C, Horgan T, Koch E, Besser RE. 2003. Legionnaires’ disease outbreak in an automobile engine manufacturing plant. J Infectious Diseases 187:10151018.[CrossRef]
68. Jernigan DB, Hofmann J, Cetron MS, Genese CA, Nuorti JP, Fields BS, Benson RF, Carter RJ, Edelstein PH, Guerrero IC, Paul SM, Lipman HB, Breiman R. 1996. Outbreak of Legionnaires’ disease among cruise ship passengers exposed to a contaminated whirlpool spa. Lancet 347:494499.[PubMed][CrossRef]
69. Travis TC, Brown EW, Peruski LF, Siludjai D, Jorakate P, Salika P, Yang G, Kozak NA, Kodani M, Warner AK, Lucas CE, Thurman KA, Winchell JM, Thamthitiwat S, Fields BS. 2012. Survey of Legionella species found in Thai soil. Int J Microbiol 2012:218791.[PubMed][CrossRef]
70. Wiedenmann A, Langhammer W, Botzenhart K. 2001. A case report of false negative Legionella test results in a chlorinated public hot water distribution system due to the lack of sodium thiosulfate in sampling bottles. Int J Hyg Envir Heal 204:245249.[CrossRef]
71. Brindle RJ, Stannett PJ, Cunliffe RN. 1987. Legionella pneumophila: comparison of isolation from water specimens by centrifugation and filtration. Epidemiol Infect 99:241247.[PubMed][CrossRef]
72. Reinthaler FF, Sattler J, Schaffler-Dullnig K, Weinmayr B, Marth E. 1993. Comparative study of procedures for isolation and cultivation of Legionella pneumophila from tap water in hospitals. J Clin Microbiol 31:12131216.[PubMed]
73. Sanden GN, Morrill WE, Fields BS, Breiman RF, Barbaree JM. 1992. Incubation of water samples containing amoebae improves detection of legionellae by the culture method. Appl Environ Microbiol 58:20012004.[PubMed]
74. Bozeman FM, Humphries JW, Campbell JM. 1968. A new group of rickettsia-like agents recovered from guinea pigs. Acta Virol 12:8793.[PubMed]
75. Hebert GA, Moss CW, McDougal LK, Bozeman FM, McKinney RM, Brenner DJ. 1980. The rickettsia-like organisms TATLOCK (1943) and HEBA (1959): bacteria phenotypically similar to but genetically distinct from Legionella pneumophila and the WIGA bacterium. Ann Intern Med 92:4552.[PubMed][CrossRef]
76. Feeley JC, Gorman GW, Weaver RE, Mackel DC, Smith HW. 1978. Primary isolation media for Legionnaires disease bacterium. J Clin Microbiol 8:320325.[PubMed]
77. Edelstein PH. 1981. Improved semiselective medium for isolation of Legionella pneumophila from contaminated clinical and environmental specimens. J Clin Microbiol 14:298303.[PubMed]
78. Pasculle AW, Feeley JC, Gibson RJ, Cordes LG, Myerowitz RL, Patton CM, Gorman GW, Carmack CL, Ezzell JW, Dowling JN. 1980. Pittsburgh pneumonia agent: direct isolation from human lung tissue. J Infect Dis 141:727732.[PubMed][CrossRef]
79. Morrill WE, Barbaree JM, Fields BS, Sanden GN, Martin WT. 1990. Increased recovery of Legionella micdadei and Legionella bozemanii on buffered charcoal yeast extract agar supplemented with albumin. J Clini Microbiol 28:61668.
80. Vickers RM, Stout JE, Yu VL, Rihs JD. 1987. Manual of culture methodology for Legionella. Sem Resp Infect 2:274279.
81. Vickers RM, Brown A, Garrity GM. 1981. Dye-containing buffered charcoal-yeast extract medium for differentiation of members of the family Legionellaceae. J Clini Microbiol 13:380382.
82. Luck C, Fry NK, Helbig JH, Jarraud S, Harrison TG. 2013. Typing methods for Legionella. Meth Mol Biol 954:119148.[CrossRef]
83. DeLuca G, Stampi S, Lezzi L, Zanetti F. 1999. Effect of heat and acid decontamination treatments on the recovery of Legionella pneumophila from drinking water using two selective media. New Microbiol 22:203208.[PubMed]
84. Ta AC, Stout JE, Yu VL, Wagener MM. 1995. Comparison of culture methods for monitoring Legionella species in hospital potable water systems and recommendations for standardization of such methods. J Clin Microbiol 33:21182123.[PubMed]
85. Amemura-Maekawa J, Hayakawa Y, Sugie H, Moribayashi A, Kura F, Chang B, Wada A, Watanabe H. 2004. Legioliulin, a new isocoumarin compound responsible for blue-white autofluorescence in Legionella (Fluoribacter) dumoffii under long-wavelength UV light. Biochem Biophys Res Commun 323:954959.[PubMed][CrossRef]
86. Wilkinson IJ, Sangster N, Ratcliff RM, Mugg PA, Davos DE, Lanser JA. 1990. Problems associated with identification of Legionella species from the environment and isolation of six possible new species. Appl Environ Microbiol 56:796802.[PubMed]
87. Orrison LH, Cherry WB, Tyndall RL, Fliermans CB, Gough SB, Lambert MA, McDougal LK, Bibb WF, Brenner DJ. 1983. Legionella oakridgensis: unusual new species isolated from cooling tower water. Appl Environ Microbiol 45:536545.[PubMed]
88. Thacker WL, Wilkinson HW, Benson RF. 1983. Comparison of slide agglutination test and direct immunofluorescence assay for identification of Legionella isolates. J Clin Microbiol 18:11131118.[PubMed]
89. Diogo A, Verissimo A, Nobre MF, da Costa MS. 1999. Usefulness of fatty acid composition for differentiation of Legionella species. J Clin Microbiol 37:22482254.[PubMed]
90. Yong SF, Tan SH, Wee J, Tee JJ, Sansom FM, Newton HJ, Hartland EL. 2010. Molecular detection of Legionella: moving on from mip. Front Microbiol 1:123.[PubMed][CrossRef]
91. Benson RF, Fields BS. 1998. Classification of the genus Legionella. Sem Resp Infect 13:9099.
92. Joly JR, McKinney RM, Tobin JO, Bibb WF, Watkins ID, Ramsay D. 1986. Development of a standardized subgrouping scheme for Legionella pneumophila serogroup 1 using monoclonal antibodies. J Clin Microbiol 23:768771.[PubMed]
93. Boissinot M, Ramsay D, Barthe C, Joly JR. 1987. Antigenic variability of the outer membrane antigens of Legionella pneumophila serogroups 1 to 8. Can J Microbiol 33:607613.[PubMed][CrossRef]
94. Ratcliff RM, Lanser JA, Manning PA, Heuzenroeder MW. 1998. Sequence-based classification scheme for the genus Legionella targeting the mip gene. J Clin Microbiol 36:15601567.[PubMed]
95. Ratcliff RM. 2013. Sequence-based identification of Legionella. Meth Mol Biol 954:5772.[CrossRef]
96. Guyard C, Low DE. 2011. Legionella infections and travel associated legionellosis. Travel Med Infect Dis 9:176186.[PubMed][CrossRef]
97. Luck PC, Helbig JH, Ehret W, Marre R, Witzleb W. 1992. Subtyping of Legionella pneumophila serogroup 1 strains isolated in Germany using monoclonal antibodies. Zentral Bakteriol 277:179187.[CrossRef]
98. Scaturro M, Losardo M, De Ponte G, Ricci ML. 2005. Comparison of three molecular methods used for subtyping of Legionella pneumophila strains isolated during an epidemic of legionellosis in Rome. J Clin Microbiol 43:53485350.[PubMed][CrossRef]
99. Jonas D, Meyer HG, Matthes P, Hartung D, Jahn B, Daschner FD, Jansen B. 2000. Comparative evaluation of three different genotyping methods for investigation of nosocomial outbreaks of Legionnaires’ disease in hospitals. J Clin Microbiol 38:22842291.[PubMed]
100. Farhat C, Mentasti M, Jacobs E, Fry NK, Luck C. 2011. The N-acylneuraminate cytidyltransferase gene, neuA, is heterogenous in Legionella pneumophila strains but can be used as a marker for epidemiological typing in the consensus sequence-based typing scheme. J Clin Microbiol 49:40524058.[PubMed][CrossRef]
101. Ratzow S, Gaia V, Helbig JH, Fry NK, Luck PC. 2007. Addition of neuA, the gene encoding N-acylneuraminate cytidylyl transferase, increases the discriminatory ability of the consensus sequence-based scheme for typing Legionella pneumophila serogroup 1 strains. J Clin Microbiol 45:19651968.[PubMed][CrossRef]
102. Fry NK, Bangsborg JM, Bergmans A, Bernander S, Etienne J, Franzin L, Gaia V, Hasenberger P, Baladron Jimenez B, Jonas D, Lindsay D, Mentula S, Papoutsi A, Struelens M, Uldum SA, Visca P, Wannet W, Harrison TG. 2002. Designation of the European Working Group on Legionella Infection (EWGLI) amplified fragment length polymorphism types of Legionella pneumophila serogroup 1 and results of intercentre proficiency testing Using a standard protocol. Eur J Clin Microbiol Infect Dis 21:722728.[PubMed][CrossRef]
103. Bates MN, Maas E, Martin T, Harte D, Grubner M, Margolin T. 2000. Investigation of the prevalence of Legionella species in domestic hot water systems. N Z Med J 113:218220.[PubMed]
104. Nazarian EJ, Bopp DJ, Saylors A, Limberger RJ, Musser KA. 2008. Design and implementation of a protocol for the detection of Legionella in clinical and environmental samples. Diagn Microbiol Infect Dis 62:125132.[PubMed][CrossRef]
105. Miller LA, Beebe JL, Butler JC, Martin W, Benson R, Hoffman RE, Fields BS. 1993. Use of polymerase chain reaction in an epidemiologic investigation of Pontiac fever. J Infect Dise 168:769772.[CrossRef]
106. Chang CW, Chou FC. 2011. Methodologies for quantifying culturable, viable, and total Legionella pneumophila in indoor air. Indoor Air 21:291299.[PubMed][CrossRef]
107. Joly P, Falconnet PA, Andre J, Weill N, Reyrolle M, Vandenesch F, Maurin M, Etienne J, Jarraud S. 2006. Quantitative real-time Legionella PCR for environmental water samples: data interpretation. Appl Environ Microbiol 72:28012808.[PubMed][CrossRef]
108. Dupuy M, Mazoua S, Berne F, Bodet C, Garrec N, Herbelin P, Menard-Szczebara F, Oberti S, Rodier MH, Soreau S, Wallet F, Hechard Y. 2011. Efficiency of water disinfectants against Legionella pneumophila and Acanthamoeba. Water Res 45:10871094.[PubMed][CrossRef]
109. Loret JF, Robert S, Thomas V, Cooper AJ, McCoy WF, Levi Y. 2005. Comparison of disinfectants for biofilm, protozoa and Legionella control. J Water Health 3:423433.[PubMed][CrossRef]
110. Krojgaard LH, Krogfelt KA, Albrechtsen HJ, Uldum SA. 2011. Cluster of Legionnaires disease in a newly built block of flats, Denmark, December 2008–January 2009. Euro Surveill 16:19759.[PubMed]
111. Mouchtouri VA, Goutziana G, Kremastinou J, Hadjichristodoulou C. 2010. Legionella species colonization in cooling towers: risk factors and assessment of control measures. Am J Infec Cont 38:5055.[CrossRef]
112. Hambidge A. 2001. Reviewing efficacy of alternative water treatment techniques—Part 2. Health Estate 55:2426.[PubMed]
113. Hambidge A. 2001. Reviewing efficacy of alternative water treatment techniques. Health Estate 55:2325.[PubMed]
114. Ricketts KD, Joseph CA EWGLI. 2006. The impact of new guidelines in Europe for the control and prevention of travel-associated Legionnaires’ disease. Int J Hyg Envir Health 209:547552.[CrossRef]
115. Fields BS, Moore MR. 2006. Control of legionellae in the environment: a guide to the US guidelines. ASHRAE Trans 112:691699.
116. Wingender J, Flemming HC. 2011. Biofilms in drinking water and their role as reservoir for pathogens. Int J Hyg Envir Health 214:417423.[CrossRef]
117. Declerck P. 2010. Biofilms: the environmental playground of Legionella pneumophila. Environ Microbiol 12:557566.[PubMed][CrossRef]
118. Pagnier I, Merchat M, La Scola B. 2009. Potentially pathogenic amoeba-associated microorganisms in cooling towers and their control. Fut Microbiol 4:615629.[CrossRef]
119. Berry D, Xi C, Raskin L. 2006. Microbial ecology of drinking water distribution systems. Curr Opin Biotech 17:297302.[PubMed][CrossRef]
120. Marchesi I, Cencetti S, Marchegiano P, Frezza G, Borella P, Bargellini A. 2012. Control of Legionella contamination in a hospital water distribution system by monochloramine. Am J Infect Cont 40:279281.[CrossRef]
121. Moore MR, Pryor M, Fields B, Lucas C, Phelan M, Besser RE. 2006. Introduction of monochloramine into a municipal water system: impact on colonization of buildings by Legionella spp. Appl Environ Microbiol 72:378383.[PubMed][CrossRef]
122. Pryor M, Springthorpe S, Riffard S, Brooks T, Huo Y, Davis G, Sattar SA. 2004. Investigation of opportunistic pathogens in municipal drinking water under different supply and treatment regimes. Water Sci Technol 50:8390.[PubMed]
123. ASHRAE. 2000. ASHRAE Guideline 12–2000—Minimizing the Risk of Legionellosis Associated with Building Water Systems. Report No. 12-2000. American Society for Heating, Refrigeration, and Air Conditioning Engineers, Atlanta, GA.
124. Mastro TD, Fields BS, Breiman RF, Campbell J, Plikaytis BD, Spika JS. 1991. Nosocomial Legionnaires’ disease and use of medication nebulizers. J Infect Dis 163:667671.[PubMed][CrossRef]
125. CTI. 2012. Legionellosis Guideline: Best Practices for Control of Legionella (WTP-148) (08). Cooling Technology Institute. Available from: http://www.cti.org/cgi-bin/download.pl.
126. CDC. 2012. Disinfection of hot tubs contaminated with Legionella. Available from: http://www.cdc.gov/legionella/files/hot-tub-disinfection.pdf.
127. CDC. 2007. Surveillance for travel-associated legionnaires disease—United States, 2005–2006. Morbid Mortal Weekly Rep 56:1261123.
128. Perola O, Kauppinen J, Kusnetsov J, Karkkainen UM, Luck PC, Katila ML. 2005. Persistent Legionella pneumophila colonization of a hospital water supply: efficacy of control methods and a molecular epidemiological analysis. APMIS 113:4553.[PubMed][CrossRef]
129. Marchesi I, Marchegiano P, Bargellini A, Cencetti S, Frezza G, Miselli M, Borella P. 2011. Effectiveness of different methods to control Legionella in the water supply: ten-year experience in an Italian university hospital. J Hosp Infect 77:4751.[PubMed][CrossRef]
130. Molloy SL, Ives R, Hoyt A, Taylor R, Rose JB. 2008. The use of copper and silver in carbon point-of-use filters for the suppression of Legionella throughput in domestic water systems. J Appl Microbiol 104:9981007.[PubMed][CrossRef]
131. Daeschlein G, Kruger WH, Selepko C, Rochow M, Dolken G, Kramer A. 2007. Hygienic safety of reusable tap water filters (Germlyser) with an operating time of 4 or 8 weeks in a haematological oncology transplantation unit. BMC Infect Dis 7:45.[PubMed][CrossRef]
132. Sheffer PJ, Stout JE, Wagener MM, Muder RR. 2005. Efficacy of new point-of-use water filter for preventing exposure to Legionella and waterborne bacteria. Am J Infect Cont 33(5 Suppl 1):S20S25.[CrossRef]

Tables

Generic image for table
TABLE 1

Characteristic of Legionnaires’ disease and Pontiac fever

Citation: Lucas C, Fields B. 2016. and Legionnaires’ Disease, p 3.2.9-1-3.2.9-13. In Yates M, Nakatsu C, Miller R, Pillai S (ed), Manual of Environmental Microbiology, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818821.ch3.2.9
Generic image for table
TABLE 2

Sources known to transmit via aerosols

Citation: Lucas C, Fields B. 2016. and Legionnaires’ Disease, p 3.2.9-1-3.2.9-13. In Yates M, Nakatsu C, Miller R, Pillai S (ed), Manual of Environmental Microbiology, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818821.ch3.2.9
Generic image for table
TABLE 3

Components and supplements of BCYE agar for culturing from the environment

Citation: Lucas C, Fields B. 2016. and Legionnaires’ Disease, p 3.2.9-1-3.2.9-13. In Yates M, Nakatsu C, Miller R, Pillai S (ed), Manual of Environmental Microbiology, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818821.ch3.2.9

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