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Chapter 3.4.3 : Overview of Microbial Source Tracking Methods Targeting Human Fecal Pollution Sources

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Abstract:

Human fecal waste is thought to contain the largest number of human pathogens representing the greatest public health risk in comparison to other fecal sources. Human fecal pollution can be introduced into water resources from damaged sewer lines, faulty septic systems, combined sewer overflows, illicit dumping activities, and even recreational bathers themselves. Ensuring public safety and adequate water quality therefore requires methods that can confirm the presence or absence, as well as, determine the concentration human fecal pollution sources. This chapter provides an overview of human-associated fecal source identification methods commonly used to assess water quality. In addition to describing various methods, this chapter will also discuss factors to consider for method selection.

Citation: Shanks O, Green H, Korajkic A, Field K. 2016. Overview of Microbial Source Tracking Methods Targeting Human Fecal Pollution Sources, p 3.4.3-1-3.4.3-8. In Yates M, Nakatsu C, Miller R, Pillai S (ed), Manual of Environmental Microbiology, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818821.ch3.4.3
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Tables

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TABLE 1

Selected PCR-based human-associated MST methods targeting bacterial genes

Citation: Shanks O, Green H, Korajkic A, Field K. 2016. Overview of Microbial Source Tracking Methods Targeting Human Fecal Pollution Sources, p 3.4.3-1-3.4.3-8. In Yates M, Nakatsu C, Miller R, Pillai S (ed), Manual of Environmental Microbiology, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818821.ch3.4.3
Generic image for table
TABLE 2

Selected human-associated MST methods targeting viruses

Citation: Shanks O, Green H, Korajkic A, Field K. 2016. Overview of Microbial Source Tracking Methods Targeting Human Fecal Pollution Sources, p 3.4.3-1-3.4.3-8. In Yates M, Nakatsu C, Miller R, Pillai S (ed), Manual of Environmental Microbiology, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818821.ch3.4.3
Generic image for table
TABLE 3

MST applications

Citation: Shanks O, Green H, Korajkic A, Field K. 2016. Overview of Microbial Source Tracking Methods Targeting Human Fecal Pollution Sources, p 3.4.3-1-3.4.3-8. In Yates M, Nakatsu C, Miller R, Pillai S (ed), Manual of Environmental Microbiology, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555818821.ch3.4.3

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