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Chapter 16 : Metabolomics of Central Carbon Metabolism in

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Abstract:

Central carbon metabolism (CCM)—defined as the enzymatic transformation of carbon through glycolysis, the pentose phosphate pathway (PPP), the citric acid cycle, the glyoxylate shunt, the methylcitrate cycle, and gluconeogenesis—is a core feature of all cells that is used to provide energy, in the form of reducing equivalents and ATP, and essential biosynthetic precursors ( Fig. 1 ). Remarkably, the same metabolic enzymes found in bacteria are also present in mammals, suggesting that the pathways of CCM have been fundamentally conserved. Intensive study has thus focused on the metabolic network of as a model system with which to understand its basic principles. However, cells vary in their specific metabolic needs according to the selective pressures they encounter. Accordingly, growing evidence has established that metabolic enzymes are often capable of operating in a diverse array of configurations ( ).

Citation: Baughn A, Rhee K. 2014. Metabolomics of Central Carbon Metabolism in , p 323-339. In Hatfull G, Jacobs W (ed), Molecular Genetics of Mycobacteria, Second Edition. ASM Press, Washington, DC. doi: 10.1128/microbiolspec.MGM2-0026-2013

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Figure 1

Schematic representation of the CCM network of based on bioinformatic reconstruction and published literature. Reversible steps of glycolysis/gluconeogenesis are shown in purple, dedicated steps of glycolysis are shown in light blue, dedicated steps of gluconeogenesis are shown in pink, the PPP is shown in red, the TCA cycle is shown in green, the glyoxylate shunt is shown in orange, and the methylcitrate cycle is shown in dark blue. The dotted green line indicates a standing question of connectivity in the TCA cycle via ketoglutarate ferredoxin oxidoreductase. Common enzyme names are shown next to the reactions they catalyze. Gene symbols are shown in parentheses. doi:10.1128/microbiolspec.MGM2-0026-2013.f1

Citation: Baughn A, Rhee K. 2014. Metabolomics of Central Carbon Metabolism in , p 323-339. In Hatfull G, Jacobs W (ed), Molecular Genetics of Mycobacteria, Second Edition. ASM Press, Washington, DC. doi: 10.1128/microbiolspec.MGM2-0026-2013
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