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Chapter 5 : Replication and Maintenance of Linear Phage-Plasmid N15

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Replication and Maintenance of Linear Phage-Plasmid N15, Page 1 of 2

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Abstract:

All cells with linear chromosomes must employ special mechanisms to replicate the extreme termini of their chromosomes, since DNA polymerases alone are unable to perform this function ( ). Most eukaryotes have open-ended DNA and employ special “telomerase” enzymes for this purpose, but there are other solutions that ensure complete replication of linear DNA: protein priming, recombination, and covalently closed terminal hairpins (reviewed in reference ). Prokaryotes usually posses circular plasmids and chromosomes, but examples of linear replicons are known. Bacteriophage N15 belongs to the small group of organisms known to replicate as linear DNA with covalently closed telomeres. Besides N15 and related phage-plasmids, only a few examples of such replicons from bacteria are known, including the linear plasmids and chromosomes common in the spirochete genus ( ) and one of the two chromosomes of ( ). In this review I will summarize the most relevant work on N15 and related phages, with a special emphasis on the mechanism of replication, generation of hairpin telomeres, control of lysogeny, and plasmid prophage maintenance.

Citation: Ravin N. 2015. Replication and Maintenance of Linear Phage-Plasmid N15, p 71-82. In Tolmasky M, Alonso J (ed), Plasmids: Biology and Impact in Biotechnology and Discovery. ASM Press, Washington, DC. doi: 10.1128/microbiolspec.PLAS-0032-2014

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Figures

Image of Figure 1
Figure 1

(A) Mechanism of conversion of phage DNA into linear plasmid. , , single stranded cohesive ends; , site after annealing and ligation of cohesive ends; , uncut target site of protelomerase; and , left and right hairpin ends of the prophage created by protelomerase. (B) Hairpin formation reaction by the protelomerase. The positions of the cleavage sites are marked by a filled triangle. Catalytically active tyrosine is shown by a hexagon; the direction of refolding of single-stranded ends is shown by a dotted arrow. The protelomerase is shown by a gray oval. doi:10.1128/microbiolspec.PLAS-0032-2014.f1

Citation: Ravin N. 2015. Replication and Maintenance of Linear Phage-Plasmid N15, p 71-82. In Tolmasky M, Alonso J (ed), Plasmids: Biology and Impact in Biotechnology and Discovery. ASM Press, Washington, DC. doi: 10.1128/microbiolspec.PLAS-0032-2014
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Image of Figure 2
Figure 2

(A) Map of N15 plasmid prophage. The N15 linear prophage is shown with a scale in kilobase pairs. Rectangles immediately above and below the scale represent predicted genes that are transcribed rightward and leftward, respectively; their colors indicate functional assignments in the following way: genes encoding terminase and virion proteins (yellow), genes responsible for plasmid maintenance (green), genes responsible for the control of lysogeny (red), other genes with predicted functions (gray), and genes with unknown functions (white). The N15 gene names are given within or near the rectangles, and alternate descriptive names are indicated above or below. Asterisks (*) mark the position of the centromere sites involved in plasmid partition. , replication initiation site. (B) Mosaic relationship between the whole genomes of phages λ, N15, ɸKO2, and PY54. Shaded areas between the genomes indicate the main regions of homology. The end of each phage’s circularly permuted virion genome (cosRL) is marked by a black vertical line. doi:10.1128/microbiolspec.PLAS-0032-2014.f2

Citation: Ravin N. 2015. Replication and Maintenance of Linear Phage-Plasmid N15, p 71-82. In Tolmasky M, Alonso J (ed), Plasmids: Biology and Impact in Biotechnology and Discovery. ASM Press, Washington, DC. doi: 10.1128/microbiolspec.PLAS-0032-2014
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Image of Figure 3
Figure 3

Three lysogeny control regions. Protein-encoding genes are shown by gray boxes; , which encodes an RNA, by an open box across the main line. Bent arrows indicate promoters (P). The positions of transcription terminators (T) are shown by solid triangles. CB binding sites () are shown by black rectangles; the LexA binding site at P471, by a black circle. Genes and sites shown above (below) the main line apply to transcription from left to right (right to left). doi:10.1128/microbiolspec.PLAS-0032-2014.f3

Citation: Ravin N. 2015. Replication and Maintenance of Linear Phage-Plasmid N15, p 71-82. In Tolmasky M, Alonso J (ed), Plasmids: Biology and Impact in Biotechnology and Discovery. ASM Press, Washington, DC. doi: 10.1128/microbiolspec.PLAS-0032-2014
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Image of Figure 4
Figure 4

Model of the N15 plasmid prophage and lytic replication. A indicates replication of the N15 plasmid prophage. B shows lytic replication initiated in the lysogen. Note that the circular head-to-head dimer is supposed to be processed by protelomerase into two circular monomers (only one is shown). The known or suggested participation of the N15 proteins TelN and RepA at the individual steps is shown. doi:10.1128/microbiolspec.PLAS-0032-2014.f4

Citation: Ravin N. 2015. Replication and Maintenance of Linear Phage-Plasmid N15, p 71-82. In Tolmasky M, Alonso J (ed), Plasmids: Biology and Impact in Biotechnology and Discovery. ASM Press, Washington, DC. doi: 10.1128/microbiolspec.PLAS-0032-2014
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Image of Figure 5
Figure 5

The maximum likelihood trees were calculated from the multiple sequence alignments of (A) RepA or (B) TelN proteins. The numbers above the nodes indicate bootstrap support values. The trees are drawn to scale, with branch lengths measured by the number of substitutions per site. doi:10.1128/microbiolspec.PLAS-0032-2014.f5

Citation: Ravin N. 2015. Replication and Maintenance of Linear Phage-Plasmid N15, p 71-82. In Tolmasky M, Alonso J (ed), Plasmids: Biology and Impact in Biotechnology and Discovery. ASM Press, Washington, DC. doi: 10.1128/microbiolspec.PLAS-0032-2014
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