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Appendix 2 : APPENDIX 2 Flowcharts and Staining Tables for Diagnostic Procedures

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APPENDIX 2 Flowcharts and Staining Tables for Diagnostic Procedures, Page 1 of 2

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Abstract:

APPENDIX 2 Flowcharts and Staining Tables for Diagnostic Procedures

Citation: Garcia L. 2016. APPENDIX 2 Flowcharts and Staining Tables for Diagnostic Procedures, p 0-1. In Diagnostic Medical Parasitology, Sixth Edition. ASM Press, Washington, DC.
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Image of Flowchart A2.1
Flowchart A2.1

Procedure for processing fresh stool for the ova and parasite examination. Schaudinn's fixative can be prepared using the mercuric chloride base or the zinc sulfate base; the copper sulfate base does not produce organism morphologic quality equal to that seen with the mercuric chloride or zinc sulfate bases. The permanent stained smear can be stained using iron hematoxylin or trichrome stains. This approach includes the direct wet smear, concentration, and the permanent stained smear (complete ova and parasite examination for fresh specimen that is liquid or very soft).

Citation: Garcia L. 2016. APPENDIX 2 Flowcharts and Staining Tables for Diagnostic Procedures, p 0-1. In Diagnostic Medical Parasitology, Sixth Edition. ASM Press, Washington, DC.
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Image of Flowchart A2.2
Flowchart A2.2

Procedure for processing liquid specimens for the ova and parasite examination. If the volume of the liquid stool is quite small, mixing some of the specimen with polyvinyl alcohol (PVA) fixative (Schaudinn's fixative or other fixative containing PVA plastic powder that serves as the adhesive to “glue” the fecal material onto the glass slide) directly on the slide is recommended. Make sure that the slide is thoroughly dry before staining.

Citation: Garcia L. 2016. APPENDIX 2 Flowcharts and Staining Tables for Diagnostic Procedures, p 0-1. In Diagnostic Medical Parasitology, Sixth Edition. ASM Press, Washington, DC.
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Image of Flowchart A2.3
Flowchart A2.3

Procedure for processing preserved stool for the ova and parasite examination by using the traditional two-vial collection kit. This collection kit has been in use for many years. If the fecal specimen arrives in the laboratory in preservative, the direct wet mount for motility should not be performed. The complete ova and parasite examination consists of the concentration and permanent stained smear. PVA, polyvinyl alcohol; SAF, sodium acetate-acetic acid-formalin.

Citation: Garcia L. 2016. APPENDIX 2 Flowcharts and Staining Tables for Diagnostic Procedures, p 0-1. In Diagnostic Medical Parasitology, Sixth Edition. ASM Press, Washington, DC.
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Image of Flowchart A2.4
Flowchart A2.4

Procedure for processing sodium acetate-acetic acid-formalin (SAF)-preserved stool for the ova and parasite examination. SAF-preserved stool can be used with the fecal immunoassay kits (fluorescent-antibody assay, enzyme immunoassay, and cartridge formats for and spp.; kits for the true pathogen or the group require fresh or frozen stools); modified acid-fast stains for coccidia and modified trichrome stains can also be performed from SAF-preserved stool.

Citation: Garcia L. 2016. APPENDIX 2 Flowcharts and Staining Tables for Diagnostic Procedures, p 0-1. In Diagnostic Medical Parasitology, Sixth Edition. ASM Press, Washington, DC.
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Image of Flowchart A2.5
Flowchart A2.5

Use of various fixatives and their recommended stains. Fecal specimens preserved using polyvinyl alcohol (PVA) fixative (liquid fixative into which PVA plastic powder has been added) can be stained using either the iron hematoxylin or trichrome stains. However, fecal specimens preserved using sodium acetate-acetic acid-formalin appear to stain better using the iron hematoxylin approach. The single-vial preservatives (proprietary formulas) can be stained with iron hematoxylin, trichrome, or a manufacturer's stain prepared to accompany a particular fixative.

Citation: Garcia L. 2016. APPENDIX 2 Flowcharts and Staining Tables for Diagnostic Procedures, p 0-1. In Diagnostic Medical Parasitology, Sixth Edition. ASM Press, Washington, DC.
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Image of Flowchart A2.6
Flowchart A2.6

Use of various fixatives and their recommended stains. Fecal specimens preserved in the Universal Fixative, TOTAL-FIX, can be stained using trichrome or iron hematoxylin. Using this processing algorithm, modified acid-fast stains (coccidia) and modified trichrome stains (microsporidia) can also be performed.

Citation: Garcia L. 2016. APPENDIX 2 Flowcharts and Staining Tables for Diagnostic Procedures, p 0-1. In Diagnostic Medical Parasitology, Sixth Edition. ASM Press, Washington, DC.
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References

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Tables

Generic image for table
Steps in the trichrome staining procedure (mercuric chloride-based PVA-preserved stool specimens)

Steps in the trichrome staining procedure (mercuric chloride-based PVA-preserved stool specimens)

Citation: Garcia L. 2016. APPENDIX 2 Flowcharts and Staining Tables for Diagnostic Procedures, p 0-1. In Diagnostic Medical Parasitology, Sixth Edition. ASM Press, Washington, DC.
Generic image for table
Table A2.2

Steps in the trichrome staining procedure (non-mercuric chloride-based PVA-preserved stool specimens)

Citation: Garcia L. 2016. APPENDIX 2 Flowcharts and Staining Tables for Diagnostic Procedures, p 0-1. In Diagnostic Medical Parasitology, Sixth Edition. ASM Press, Washington, DC.
Generic image for table
Table A2.3

Steps in the iron hematoxylin staining procedure (mercuric chloride-based PVA-preserved stool specimens) (Spencer-Monroe method)

Citation: Garcia L. 2016. APPENDIX 2 Flowcharts and Staining Tables for Diagnostic Procedures, p 0-1. In Diagnostic Medical Parasitology, Sixth Edition. ASM Press, Washington, DC.
Generic image for table
Table A2.4

Steps in the iron hematoxylin staining procedure (mercuric chloride-based PVA-preserved stool specimens) (Tompkins-Miller method)

Citation: Garcia L. 2016. APPENDIX 2 Flowcharts and Staining Tables for Diagnostic Procedures, p 0-1. In Diagnostic Medical Parasitology, Sixth Edition. ASM Press, Washington, DC.
Generic image for table
Table A2.5

Steps in the iron hematoxylin staining procedure (incorporating the carbol fuchsin step)

Citation: Garcia L. 2016. APPENDIX 2 Flowcharts and Staining Tables for Diagnostic Procedures, p 0-1. In Diagnostic Medical Parasitology, Sixth Edition. ASM Press, Washington, DC.
Generic image for table
Table A2.6

Steps in the trichrome staining procedure (Universal Fixative [no mercury, no formalin, no PVA])

Citation: Garcia L. 2016. APPENDIX 2 Flowcharts and Staining Tables for Diagnostic Procedures, p 0-1. In Diagnostic Medical Parasitology, Sixth Edition. ASM Press, Washington, DC.
Generic image for table
Table A2.7

Oil-mounted permanent stained smears (no Permount is used)

Citation: Garcia L. 2016. APPENDIX 2 Flowcharts and Staining Tables for Diagnostic Procedures, p 0-1. In Diagnostic Medical Parasitology, Sixth Edition. ASM Press, Washington, DC.
Generic image for table
Table A2.8

Tips on stool processing and staining

Citation: Garcia L. 2016. APPENDIX 2 Flowcharts and Staining Tables for Diagnostic Procedures, p 0-1. In Diagnostic Medical Parasitology, Sixth Edition. ASM Press, Washington, DC.

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