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Appendix 9 : APPENDIX 9 Frequently Asked Questions about Diagnostic Parasitology

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APPENDIX 9 Frequently Asked Questions about Diagnostic Parasitology, Page 1 of 2

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Abstract:

APPENDIX 9 Frequently Asked Questions about Diagnostic Parasitology

Citation: Garcia L. 2016. APPENDIX 9 Frequently Asked Questions about Diagnostic Parasitology, p 0-1. In Diagnostic Medical Parasitology, Sixth Edition. ASM Press, Washington, DC.
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Figures

Image of Figure A9.1
Figure A9.1

The traditional method for preparing a thin blood film. The blood can be either “pushed” or “pulled” by the spreader slide. (Illustration by Sharon Belkin.) Note that “D” illustrates a thin film with a good feather edge. doi:10.1128/9781555819002.Apx9.f1

Citation: Garcia L. 2016. APPENDIX 9 Frequently Asked Questions about Diagnostic Parasitology, p 0-1. In Diagnostic Medical Parasitology, Sixth Edition. ASM Press, Washington, DC.
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Image of Figure A9.2
Figure A9.2

Poorly prepared thin and thick blood films (dirty slides, oil on slides, too thick preparations, poor spreading of the blood); organism morphology will be very poor on the stained films. doi:10.1128/9781555819002.Apx9.f2

Citation: Garcia L. 2016. APPENDIX 9 Frequently Asked Questions about Diagnostic Parasitology, p 0-1. In Diagnostic Medical Parasitology, Sixth Edition. ASM Press, Washington, DC.
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Image of Figure A9.3
Figure A9.3

Method of thick-thin combination blood film preparation. (a) Position of drop of EDTA-containing blood. (b) Position of the applicator stick in contact with blood and glass slide. (c) Rotation of the applicator stick. (d) Completed thick-thin combination blood film prior to staining. (Illustration by Sharon Belkin.) doi:10.1128/9781555819002.Apx9.f3

Citation: Garcia L. 2016. APPENDIX 9 Frequently Asked Questions about Diagnostic Parasitology, p 0-1. In Diagnostic Medical Parasitology, Sixth Edition. ASM Press, Washington, DC.
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Image of Figure A9.4
Figure A9.4

General diagram of a rapid malaria test. Readings: top (“Control”), the negative test shows the control line only; middle (“Panspecific AB”), control line plus the line indicates the presence of a panspecific antigen (common to all spp. but most sensitive for rather than and ); bottom (“ AB”), control line, panspecific antigen line, and line specific for antigen presence. Note: most cartridge rapid malaria tests have not been developed to detect (under development). See . doi:10.1128/9781555819002.Apx9.f4

Citation: Garcia L. 2016. APPENDIX 9 Frequently Asked Questions about Diagnostic Parasitology, p 0-1. In Diagnostic Medical Parasitology, Sixth Edition. ASM Press, Washington, DC.
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References

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1. Garcia LS (ed). 2010. Clinical Microbiology Procedures Handbook, 3rd ed, vol 2, Parasitology. ASM Press, Washington, DC.
2. Garcia LS. 2009. Practical Guide to Diagnostic Parasitology, 2nd ed. ASM Press, Washington, DC.
3. Garcia LS (coord ed). 2003. Cumitech 30A, Selection and Use of Laboratory Procedures for Diagnosis of Parasitic Infections of the Gastrointestinal Tract. ASM Press, Washington, DC.
4. National Committee for Clinical Laboratory Standards. 1997. Procedures for the Recovery and Identification of Parasites from the Intestinal Tract, 2nd ed. Approved Guideline M28-A. National Committee for Clinical Laboratory Standards, Villanova, PA.
5. Hiatt RA,, Markell EK,, Ng E. 1995. How many stool examinations are necessary to detect pathogenic intestinal protozoa? Am J Trop Med Hyg 53:3639.
6. Paustenbach D,, Alarie Y,, Kulle T,, Schachter N,, Smith R,, Swenberg J,, Witschi H,, Horowitz SB. 1997. A recommended occupational exposure limit for formaldehyde based on irritation. J Toxicol Environ Health 50:217263.
7. Code of Federal Regulations. 1987. Update 27 May 1992. Title 29, CFR 1910.1200 and 29 CFR 1910.1296. U.S. Government Printing Office, Washington, DC.
8. Code of Federal Regulations. 1989. Title 29, CFR 1910.106. U.S. Government Printing Office, Washington, DC.
9. Code of Federal Regulations. 2015. Title 29, CFR 1910.1048. U.S. Government Printing Office, Washington, DC.
10. Hanson KL,, Cartwright CP. 2001. Use of an enzyme immunoassay does not eliminate the need to analyze multiple stool specimens for sensitive detection of Giardia lamblia. J Clin Microbiol 39:474477.
11. Biswas JS,, Al-Ali A,, Raiput P,, Smith D,, Goldenberg SD. 2014. A parallel diagnostic accuracy study of three molecular panels for the detection of bacterial gastroenteritis. Eur J Clin Microbiol Infect Dis. 33:20752081.
12. Isenberg HD (ed). 2004. Clinical Microbiology Procedures Handbook, 2nd ed, vol 2, Parasitology. ASM Press, Washington, DC.
13. National Committee for Clinical Laboratory Standards. 2000. Use of Blood Film Examination for Parasites. Approved guideline M15-A, National Committee for Clinical Laboratory Standards, Villanova, PA.
14. Garcia LS (ed). 2008. Cumitech 46, Laboratory Procedures for the Diagnosis of Blood Parasites. ASM Press, Washington, DC.
15. World Health Organization. 2012. Malaria rapid diagnostic test performance. Summary results of WHO product testing of malaria RDTs: Round 1-4 (2008-2012). (http://apps.who.int/iris/bitstream/10665/77748/1/9789241504720_eng.pdf?ua=1).
16. Imwong M,, Nakeesathit S,, Day NP,, White NJ. 2011. A review of mixed malaria species infections in anopheline mosquitoes. Malar J 10:253. doi:10.1186/1475-2875-10-253.
17. Kokoskin E. 2001. The Malaria Manual. McGill University for Tropical Diseases, Montreal, Quebec, Canada.

Tables

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Stool testing order recommendations (for physicians)

Stool testing order recommendations (for physicians)

Citation: Garcia L. 2016. APPENDIX 9 Frequently Asked Questions about Diagnostic Parasitology, p 0-1. In Diagnostic Medical Parasitology, Sixth Edition. ASM Press, Washington, DC.
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Citation: Garcia L. 2016. APPENDIX 9 Frequently Asked Questions about Diagnostic Parasitology, p 0-1. In Diagnostic Medical Parasitology, Sixth Edition. ASM Press, Washington, DC.
Generic image for table
Untitled

Citation: Garcia L. 2016. APPENDIX 9 Frequently Asked Questions about Diagnostic Parasitology, p 0-1. In Diagnostic Medical Parasitology, Sixth Edition. ASM Press, Washington, DC.
Generic image for table
Untitled

Citation: Garcia L. 2016. APPENDIX 9 Frequently Asked Questions about Diagnostic Parasitology, p 0-1. In Diagnostic Medical Parasitology, Sixth Edition. ASM Press, Washington, DC.
Generic image for table
Untitled

Citation: Garcia L. 2016. APPENDIX 9 Frequently Asked Questions about Diagnostic Parasitology, p 0-1. In Diagnostic Medical Parasitology, Sixth Edition. ASM Press, Washington, DC.
Generic image for table
Untitled

Citation: Garcia L. 2016. APPENDIX 9 Frequently Asked Questions about Diagnostic Parasitology, p 0-1. In Diagnostic Medical Parasitology, Sixth Edition. ASM Press, Washington, DC.

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