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Chapter 5 : Examination of Other Specimens from the Intestinal Tract and the Urogenital System

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Examination of Other Specimens from the Intestinal Tract and the Urogenital System, Page 1 of 2

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Abstract:

  • Examination for pinworm
    • Cellulose tape preparations
    • Anal swabs
  • Sigmoidoscopy material
    • Direct saline mount
    • Permanent stained slide
  • Duodenal contents
    • Duodenal drainage
    • Duodenal capsule technique (Entero-Test)
  • Urogenital specimens
    • Trichomoniasis
    • Filariasis
    • Schistosomiasis

Citation: Garcia L. 2016. Examination of Other Specimens from the Intestinal Tract and the Urogenital System, p 98-106. In Diagnostic Medical Parasitology, Sixth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555819002.ch5
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Figures

Image of Figure 5.1
Figure 5.1

Collection of eggs by the cellulose tape method. (Illustration by Sharon Belkin.) doi:10.1128/9781555819002.ch5.f1

Citation: Garcia L. 2016. Examination of Other Specimens from the Intestinal Tract and the Urogenital System, p 98-106. In Diagnostic Medical Parasitology, Sixth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555819002.ch5
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Image of Figure 5.2
Figure 5.2

Diagram of a commercial kit (Evergreen Scientific) for use in sampling the perianal area for the presence of pinworm () eggs. On the left is the vial containing the sampler, which has sticky tape around the end. Once this is applied to the perianal area and eggs are picked up on the tape, the label area is placed at one end of the slide. The sticky tape is rolled down the slide and attaches to the glass. This device is easy to use and provides an area sufficient for adequate sampling. A minimum of four to six consecutive negative tapes are required to rule out a pinworm infection; most laboratories are accepting four rather than requesting the full six. (Illustration by Sharon Belkin.) doi:10.1128/9781555819002.ch5.f2

Citation: Garcia L. 2016. Examination of Other Specimens from the Intestinal Tract and the Urogenital System, p 98-106. In Diagnostic Medical Parasitology, Sixth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555819002.ch5
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Image of Figure 5.3
Figure 5.3

(Top) (pinworm) eggs seen in a Scotch tape preparation; note the football-shaped eggs with one side a bit flatter than the other. In some preparations, eggs are seen that contain fully developed larvae; such eggs are infective. (Middle) Adult female pinworm found on a collection device. Note the large, round esophageal bulb. (Bottom) Adult female pinworm, enlarged anterior end. Note the cephalic expansions around the end of the head, as well as the large, round esophageal bulb. doi:10.1128/9781555819002.ch5.f3

Citation: Garcia L. 2016. Examination of Other Specimens from the Intestinal Tract and the Urogenital System, p 98-106. In Diagnostic Medical Parasitology, Sixth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555819002.ch5
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Image of Figure 5.4
Figure 5.4

trophozoites stained with trichrome stain. Note the clearly defined internal details (nuclear and cytoplasmic characteristics, plus the ingested red blood cells). doi:10.1128/9781555819002.ch5.f4

Citation: Garcia L. 2016. Examination of Other Specimens from the Intestinal Tract and the Urogenital System, p 98-106. In Diagnostic Medical Parasitology, Sixth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555819002.ch5
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Image of Figure 5.5
Figure 5.5

Duodenal aspirate specimens. (Top) in mucus from a duodenal aspirate stained with trichrome stain. Note the teardrop trophozoites (from the front view) and the darker-staining trophozoites that resemble the curved part of a spoon (side view). Also notice the number of organisms contained within the mucus. (Middle) A rhabditiform larva in a wet mount. The genital primordial packet of cells is visible about halfway down the larva on the left side. (Bottom) spp. oocysts in duodenal aspirate material stained with a modified acid-fast stain. doi:10.1128/9781555819002.ch5.f5

Citation: Garcia L. 2016. Examination of Other Specimens from the Intestinal Tract and the Urogenital System, p 98-106. In Diagnostic Medical Parasitology, Sixth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555819002.ch5
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Image of Figure 5.6
Figure 5.6

Entero-Test capsule for sampling duodenal contents (HCD; Nutri-Link Ltd., Newton Abbot, United Kingdom; adult and pediatric capsules). The device consists of a length of nylon yarn coiled inside a gelatin capsule. The yarn protrudes through one end of the capsule; this end of the line is taped to the side of the patient's face. The capsule is then swallowed, the gelatin dissolves in the stomach, and the weighted string is carried by peristalsis into the duodenum. (Illustration by Nobuko Kitamura.) doi:10.1128/9781555819002.ch5.f6

Citation: Garcia L. 2016. Examination of Other Specimens from the Intestinal Tract and the Urogenital System, p 98-106. In Diagnostic Medical Parasitology, Sixth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555819002.ch5
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Image of Figure 5.7
Figure 5.7

Entero-Test string test for sampling duodenal contents. (A) Capsule with partially extracted string; (B) capsule with string before being swallowed; (C) end of the string taped to the cheek. (From Leodolter A et al, 584–586, 2005.) doi:10.1128/9781555819002.ch5.f7

Citation: Garcia L. 2016. Examination of Other Specimens from the Intestinal Tract and the Urogenital System, p 98-106. In Diagnostic Medical Parasitology, Sixth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555819002.ch5
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Image of Figure 5.8
Figure 5.8

(Top) trophozoite. (Illustration by Sharon Belkin.) (Middle) trophozoites seen in a wet mount preparation. (Bottom, left) (stool); (right) (urinary-genital tract). doi:10.1128/9781555819002.ch5.f8

Citation: Garcia L. 2016. Examination of Other Specimens from the Intestinal Tract and the Urogenital System, p 98-106. In Diagnostic Medical Parasitology, Sixth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555819002.ch5
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Image of Figure 5.9
Figure 5.9

Membrane filtration system. (Upper) Membrane holder, which can be attached to a syringe for filtration of various types of specimens, particularly urine. (Lower) Package of membranes; different sizes with various mesh sizes are available, depending on the clinical specimen and suspected organism size. doi:10.1128/9781555819002.ch5.f9

Citation: Garcia L. 2016. Examination of Other Specimens from the Intestinal Tract and the Urogenital System, p 98-106. In Diagnostic Medical Parasitology, Sixth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555819002.ch5
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Image of Figure 5.10
Figure 5.10

eggs from a urine filtration. Note the terminal spines on the eggs. Since the specimens were not preserved prior to filtration, determination of the viability of the eggs is possible. The viability information should be conveyed to the physician as a part of the report. doi:10.1128/9781555819002.ch5.f10

Citation: Garcia L. 2016. Examination of Other Specimens from the Intestinal Tract and the Urogenital System, p 98-106. In Diagnostic Medical Parasitology, Sixth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555819002.ch5
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