Chapter 25 : Bloodstream Infections

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Bloodstream infections (BSIs) and sepsis rank among the top reasons for human mortality for hospitalized patients. The full breadth of sepsis syndromes are particularly dangerous to any immunocompromised patient. Sepsis is one of the oldest of medical syndromes and dates back to the time in which Hippocrates characterized it as a clinical syndrome ( ). Worldwide estimates of sepsis prevalence exceed 19 million cases per year, with over 750,000 in the United States ( ). While only 2% of patients are admitted to the hospital with severe sepsis, they represent at least 10% of all ICU admissions in the U.S. ( ).

Citation: Martinez R, Wolk D. 2016. Bloodstream Infections, p 653-689. In Hayden R, Wolk D, Carroll K, Tang Y (ed),

Diagnostic Microbiology of the Immunocompromised Host, Second Edition

. ASM Press, Washington, DC. doi: 10.1128/microbiolspec.DMIH2-0031-2016
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Figure 1

The host response in severe sepsis. The host response to sepsis is characterized by both proinflammatory responses (top of panel, in red) and anti-inflammatory immunosuppressive responses (bottom of panel, in blue). The direction, extent, and duration of these reactions are determined by both host factors (e.g., genetic characteristics, age, coexisting illnesses, and medications) and pathogen factors (e.g., microbial load and virulence). Inflammatory responses are initiated by interaction between pathogen-associated molecular patterns expressed by pathogens and pattern-recognition receptors expressed by host cells at the cell surface (toll-like receptors [TLRs] and C-type lectin receptors [CLRs]), in the endosome (TLRs) or in the cytoplasm (retinoic acid inducible gene 1-like receptors [RLRs] and nucleotide-binding oligomerization domain-like receptors [NLRs]). The consequence of exaggerated inflammation is collateral tissue damage and necrotic cell death, which results in the release of damage-associated molecular patterns, so-called danger molecules that perpetuate inflammation at least in part by acting on the same pattern-recognition receptors that are triggered by pathogens. Reprinted from reference ( ), with permission.

Citation: Martinez R, Wolk D. 2016. Bloodstream Infections, p 653-689. In Hayden R, Wolk D, Carroll K, Tang Y (ed),

Diagnostic Microbiology of the Immunocompromised Host, Second Edition

. ASM Press, Washington, DC. doi: 10.1128/microbiolspec.DMIH2-0031-2016
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Figure 2

Organ failure in severe sepsis and dysfunction of the vascular endothelium and mitochondria. Sepsis is associated with microvascular thrombosis caused by concurrent activation of coagulation (mediated by tissue factor) and impairment of anticoagulant mechanisms as a consequence of reduced activity of endogenous anticoagulant pathways (mediated by activated protein C, antithrombin, and tissue factor pathway inhibitor), plus impaired fibrinolysis owing to enhanced release of plasminogen activator inhibitor type 1 (PAI-1). The capacity to generate activated protein C is impaired at least in part by reduced expression of two endothelial receptors: thrombomodulin (TM) and the endothelial protein C receptor. Thrombus formation is further facilitated by neutrophil extracellular traps (NETs) released from dying neutrophils. Thrombus formation results in tissue hypoperfusion, which is aggravated by vasodilatation, hypotension, and reduced red-cell deformability. Tissue oxygenation is further impaired by the loss of barrier function of the endothelium owing to a loss of function of vascular endothelial (VE) cadherin, alterations in endothelial cell-to-cell tight junctions, high levels of angiopoietin 2, and a disturbed balance between sphingosine-1 phosphate receptor 1 (S1P1) and S1P3 within the vascular wall, which is at least in part due to preferential induction of S1P3 through protease-activated receptor 1 (PAR1) as a result of a reduced ratio of activated protein C to thrombin. Oxygen use is impaired at the subcellular level because of damage to mitochondria from oxidative stress. Reprinted from reference ( ), with permission.

Citation: Martinez R, Wolk D. 2016. Bloodstream Infections, p 653-689. In Hayden R, Wolk D, Carroll K, Tang Y (ed),

Diagnostic Microbiology of the Immunocompromised Host, Second Edition

. ASM Press, Washington, DC. doi: 10.1128/microbiolspec.DMIH2-0031-2016
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Figure 3

Algorithms for diagnosis, prognostication, and prediction of response to therapy. FA = fluorescent antibody stain; AFB = acid-fast bacilli; mAFB = modified AFB; SSTI = skin and soft tissue infection; LDT = Laboratory developed test.

Citation: Martinez R, Wolk D. 2016. Bloodstream Infections, p 653-689. In Hayden R, Wolk D, Carroll K, Tang Y (ed),

Diagnostic Microbiology of the Immunocompromised Host, Second Edition

. ASM Press, Washington, DC. doi: 10.1128/microbiolspec.DMIH2-0031-2016
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1. Majno G . 1991. The ancient riddle of sigma eta psi iota sigma (sepsis). J Infect Dis 163 : 937945.[PubMed] [CrossRef]
2. Adhikari NK,, Fowler RA,, Bhagwanjee S,, Rubenfeld GD . 2010. Critical care and the global burden of critical illness in adults. Lancet 376 : 13391346.[PubMed] [CrossRef]
3. Angus DC,, van der Poll T . 2013. Severe sepsis and septic shock. N Engl J Med 369 : 840851.[PubMed] [CrossRef]
4. Dellinger RP,, Levy MM,, Rhodes A,, Annane D,, Gerlach H,, Opal SM,, Sevransky JE,, Sprung CL,, Douglas IS,, Jaeschke R,, Osborn TM,, Nunnally ME,, Townsend SR,, Reinhart K,, Kleinpell RM,, Angus DC,, Deutschman CS,, Machado FR,, Rubenfeld GD,, Webb S,, Beale RJ,, Vincent JL,, Moreno R . 2013. Surviving Sepsis Campaign: international guidelines for management of severe sepsis and septic shock, 2012. Intensive Care Med 39 : 165228.[PubMed] [CrossRef]
5. Snyder SR,, Favoretto AM,, Baetz RA,, Derzon JH,, Madison BM,, Mass D,, Shaw CS,, Layfield CD,, Christenson RH,, Liebow EB . 2012. Effectiveness of practices to reduce blood culture contamination: a Laboratory Medicine Best Practices systematic review and meta-analysis. Clin Biochem 45 : 9991011.[PubMed] [CrossRef]
6. Buehler SS,, Madison B,, Snyder SR,, Derzon JH,, Cornish NE,, Saubolle MA,, Weissfeld AS,, Weinstein MP,, Liebow EB,, Wolk DM . 2016. Effectiveness of practices to increase timeliness of providing targeted therapy for inpatients with bloodstream infections: a Laboratory Medicine Best Practices systematic review and meta-analysis. Clin Microbiol Rev 29 : 59103.[PubMed] [CrossRef]
7. Rangel-Frausto MS,, Pittet D,, Costigan M,, Hwang T,, Davis CS,, Wenzel RP . 1995. The natural history of the systemic inflammatory response syndrome (SIRS). A prospective study. JAMA 273 : 117123.[PubMed] [CrossRef]
8. Cawcutt KA,, Peters SG . 2014. Severe sepsis and septic shock: clinical overview and update on management 1. Mayo Clin Proc 89 : 15721578.[PubMed] [CrossRef]
9. Kumar A,, Roberts D,, Wood KE,, Light B,, Parrillo JE,, Sharma S,, Suppes R,, Feinstein D,, Zanotti S,, Taiberg L,, Gurka D,, Kumar A,, Cheang M . 2006. Duration of hypotension before initiation of effective antimicrobial therapy is the critical determinant of survival in human septic shock. Crit Care Med 34 : 15891596.[PubMed] [CrossRef]
10. Sharma S,, Kumar A . 2008. Antimicrobial management of sepsis and septic shock. Clin Chest Med 29 : 677687. ix.[PubMed] [CrossRef]
11. Garner JS,, Jarvis WR,, Emori TG,, Horan TC,, Hughes JM . 1988. CDC definitions for nosocomial infections, 1988. Am J Infect Control 16 : 128140.[PubMed] [CrossRef]
12. van der Poll T,, Opal SM . 2008. Host–pathogen interactions in sepsis. Lancet Infect Dis 8 : 3243.[PubMed] [CrossRef]
13. Takeuchi O,, Akira S . 2010. Pattern recognition receptors and inflammation. Cell 140 : 805820.[PubMed] [CrossRef]
14. Goldenberg NM,, Steinberg BE,, Slutsky AS,, Lee WL . 2011. Broken barriers: a new take on sepsis pathogenesis. Sci Transl Med 3 : 88ps25. [PubMed] [CrossRef]
15. Zhang Q,, Raoof M,, Chen Y,, Sumi Y,, Sursal T,, Junger W,, Brohi K,, Itagaki K,, Hauser CJ . 2010. Circulating mitochondrial DAMPs cause inflammatory responses to injury. Nature 464 : 104107.[PubMed] [CrossRef]
16. Galley HF . 2011. Oxidative stress and mitochondrial dysfunction in sepsis. Br J Anaesth 107 : 5764.[PubMed] [CrossRef]
17. Ruf W . 2010. New players in the sepsis-protective activated protein C pathway. J Clin Invest 120 : 30843087.[PubMed] [CrossRef]
18. Levi M,, van der Poll T . 2010. Inflammation and coagulation. Crit Care Med 38 : S26S34.[PubMed] [CrossRef]
19. Andersson U,, Tracey KJ . 2012. Reflex principles of immunological homeostasis. Annu Rev Immunol 30 : 313335.[PubMed] [CrossRef]
20. Boomer JS,, To K,, Chang KC,, Takasu O,, Osborne DF,, Walton AH,, Bricker TL,, Jarman SD,, Kreisel D,, Krupnick AS,, Srivastava A,, Swanson PE,, Green JM,, Hotchkiss RS . 2011. Immunosuppression in patients who die of sepsis and multiple organ failure. JAMA 306 : 25942605.[PubMed] [CrossRef]
21. Carson WF,, Cavassani KA,, Dou Y,, Kunkel SL . 2011. Epigenetic regulation of immune cell functions during post-septic immunosuppression. Epigenetics 6 : 273283.[PubMed] [CrossRef]
22. Hotchkiss RS,, Tinsley KW,, Swanson PE,, Grayson MH,, Osborne DF,, Wagner TH,, Cobb JP,, Coopersmith C,, Karl IE . 2002. Depletion of dendritic cells, but not macrophages, in patients with sepsis. J Immunol 168 : 24932500.[PubMed] [CrossRef]
23. Hotchkiss RS,, Tinsley KW,, Swanson PE,, Schmieg RE,, Hui JJ,, Chang KC,, Osborne DF,, Freeman BD,, Cobb JP,, Buchman TG,, Karl IE . 2001. Sepsis-induced apoptosis causes progressive profound depletion of B and CD4(+) T lymphocytes in humans. J Immunol 166 : 69526963.[PubMed] [CrossRef]
24. Ramphal R . 2004. Changes in the etiology of bacteremia in febrile neutropenic patients and the susceptibilities of the currently isolated pathogens 2. Clin Infect Dis 39(Suppl 1): S25S31.[PubMed] [CrossRef]
25. Klastersky J,, Zinner SH,, Calandra T,, Gaya H,, Glauser MP,, Meunier F,, Rossi M,, Schimpff SC,, Tattersall M,, Viscoli C . 1988. Empiric antimicrobial therapy for febrile granulocytopenic cancer patients: lessons from four EORTC trials. Eur J Cancer Clin Oncol 24(Suppl 1): S35S45.[PubMed] [CrossRef]
26. Feliciano R,, Swedler W,, Varga J . 1999. Infection with uncommon subgroup Y Neisseria meningitidis in patients with systemic lupus erythematosus. Clin Exp Rheumatol 17 : 737740.[PubMed]
27. Papaparaskevas J,, Katsandri A,, Pantazatou A,, Stefanou I,, Avlamis A,, Legakis NJ,, Tsakris A . 2011. Epidemiological characteristics of infections caused by Bacteroides, Prevotella and Fusobacterium species: A prospective observational study. Anaerobe 17 : 113117.[PubMed] [CrossRef]
28. Suzuki H,, Murata K,, Sakamoto A . 2009. Fulminant sepsis due to anaerobic bacterial infection in immuno-compromised state. Leg Med 11 : 237240.[PubMed] [CrossRef]
29. Forrester JD,, Spain DA . 2014. Clostridium ramosum bacteremia: case report and literature review. Surg Infect (Larchmt) 15 : 343346.[PubMed] [CrossRef]
30. Lassmann B,, Gustafson DR,, Wood CM,, Rosenblatt JE . 2007. Reemergence of anaerobic bacteremia. Clin Infect Dis 44 : 895900.[PubMed] [CrossRef]
31. Wilson JR,, Limaye AP . 2004. Risk factors for mortality in patients with anaerobic bacteremia. Eur J Clin Microbiol Infect Dis 23 : 310316.[PubMed] [CrossRef]
32. von Gottberg A,, Sacks L,, Machala S,, Blumberg L . 2001. Utility of blood cultures and incidence of mycobacteremia in patients with suspected tuberculosis in a South African infectious disease referral hospital. Int J Tuberc Lung Dis 5 : 8086.[PubMed]
33. Doucette K,, Fishman JA . 2004. Nontuberculous mycobacterial infection in hematopoietic stem cell and solid organ transplant recipients. Clin Infect Dis 38 : 14281439.[PubMed] [CrossRef]
34. Somily AM,, Al-Anazi AR,, Babay HA,, Al-Aska AI,, Al-Hedaithy MA,, Al-Hamoudi WK,, Al Boukai AA,, Sabri MS,, AlThawadi SI,, Kambal AM . 2010. Mycobacteriumchelonae complex bacteremia from a post-renal transplant patient: case report and literature review. Jpn J Infect Dis 63 : 6164.[PubMed]
35. Tagashira Y,, Kozai Y,, Yamasa H,, Sakurada M,, Kashiyama T,, Honda H . 2015. A cluster of central line-associated bloodstream infections due to rapidly growing nontuberculous mycobacteria in patients with hematologic disorders at a Japanese tertiary care center: an outbreak investigation and review of the literature. Infect Control Hosp Epidemiol 36 : 7680.[PubMed] [CrossRef]
36. Archibald LK,, McDonald LC,, Rheanpumikankit S,, Tansuphaswadikul S,, Chaovanich A,, Eampokalap B,, Banerjee SN,, Reller LB,, Jarvis WR . 1999. Fever and human immunodeficiency virus infection as sentinels for emerging mycobacterial and fungal bloodstream infections in hospitalized patients ≥15 years old, Bangkok. J Infect Dis 180 : 8792.[PubMed] [CrossRef]
37. Oplustil CP,, Leite OH,, Oliveira MS,, Sinto SI,, Uip DE,, Boulos M,, Mendes CF . 2001. Detection of mycobacteria in the bloodstream of patients with acquired immunodeficiency syndrome in a university hospital in Brazil. Braz J Infect Dis 5 : 252259.[PubMed] [CrossRef]
38. De Groote MA,, Huitt G . 2006. Infections due to rapidly growing mycobacteria. Clin Infect Dis 42 : 17561763.[PubMed] [CrossRef]
39. Trick WE,, Fridkin SK,, Edwards JR,, Hajjeh RA,, Gaynes RP . 2002. Secular trend of hospital-acquired candidemia among intensive care unit patients in the United States during 1989-1999. Clin Infect Dis 35 : 627630.[PubMed] [CrossRef]
40. Ostrosky-Zeichner L,, Sable C,, Sobel J,, Alexander BD,, Donowitz G,, Kan V,, Kauffman CA,, Kett D,, Larsen RA,, Morrison V,, Nucci M,, Pappas PG,, Bradley ME,, Major S,, Zimmer L,, Wallace D,, Dismukes WE,, Rex JH . 2007. Multicenter retrospective development and validation of a clinical prediction rule for nosocomial invasive candidiasis in the intensive care setting. Eur J Clin Microbiol Infect Dis 26 : 271276.[PubMed] [CrossRef]
41. Blumberg HM,, Jarvis WR,, Soucie JM,, Edwards JE,, Patterson JE,, Pfaller MA,, Rangel-Frausto MS,, Rinaldi MG,, Saiman L,, Wiblin RT,, Wenzel RP . 2001. Risk factors for candidal bloodstream infections in surgical intensive care unit patients: the NEMIS prospective multicenter study. Clin Infect Dis 33 : 177186.[PubMed] [CrossRef]
42. Wisplinghoff H,, Bischoff T,, Tallent SM,, Seifert H,, Wenzel RP,, Edmond MB . 2004. Nosocomial bloodstream infections in U.S. hospitals: analysis of 24,179 cases from a prospective nationwide surveillance study. Clin Infect Dis 39 : 309317.[PubMed] [CrossRef]
43. Pappas PG,, Rex JH,, Lee J,, Hamill RJ,, Larsen RA,, Powderly W,, Kauffman CA,, Hyslop N,, Mangino JE,, Chapman S,, Horowitz HW,, Edwards JE,, Dismukes WE . 2003. A prospective observational study of candidemia: epidemiology, therapy, and influences on mortality in hospitalized adult and pediatric patients. Clin Infect Dis 37 : 634643.[PubMed] [CrossRef]
44. Pfaller MA,, Diekema DJ . 2004. Rare and emerging opportunistic fungal pathogens: concern for resistance beyond Candida albicans and Aspergillus fumigatus . J Clin Microbiol 42 : 44194431.[PubMed] [CrossRef]
45. Nucci M,, Marr KA . 2005. Emerging fungal diseases. Clin Infect Dis 41 : 521526.[PubMed] [CrossRef]
46. Petti CA,, Zaidi AK,, Mirrett S,, Reller LB . 1996. Comparison of Isolator 1.5 and BACTEC NR660 aerobic 6A blood culture systems for detection of fungemia in children. J Clin Microbiol 34 : 18771879.[PubMed]
47. Avni T,, Leibovici L,, Paul M . 2011. PCR diagnosis of invasive candidiasis: systematic review and meta-analysis. J Clin Microbiol 49 : 665670.[PubMed] [CrossRef]
48. Pfaller M,, Neofytos D,, Diekema D,, Azie N,, Meier-Kriesche HU,, Quan SP,, Horn D . 2012. Epidemiology and outcomes of candidemia in 3648 patients: data from the Prospective Antifungal Therapy (PATH Alliance®) registry, 2004-2008. Diagn Microbiol Infect Dis 74 : 323331.[PubMed] [CrossRef]
49. Biswal D,, Sahu M,, Mahajan A,, Advani SH,, Shah S . 2015. Kodameae ohmeri - an emerging yeast: two cases and literature review. J Clin Diagn Res 9 : DD01DD03.[PubMed] [CrossRef]
50. Al-Sweih N,, Khan ZU,, Ahmad S,, Devarajan L,, Khan S,, Joseph L,, Chandy R . 2011. Kodamaea ohmeri as an emerging pathogen: a case report and review of the literature. Med Mycol 49 : 766770.[PubMed]
51. Padhi S,, Dash M,, Pattanaik S,, Sahu S . 2014. Fungemia due to Trichosporonmucoides in a diabetes mellitus patient: a rare case report. Indian J Med Microbiol 32 : 7274.[PubMed] [CrossRef]
52. Capoor MR,, Aggarwal S,, Raghvan C,, Gupta DK,, Jain AK,, Chaudhary R . 2014. Clinical and microbiological characteristics of Rhodotorula mucilaginosa infections in a tertiary-care facility. Indian J Med Microbiol 32 : 304309.[PubMed] [CrossRef]
53. Monterrubio Villar J,, Gonzalez Velasco C,, Jimenez Delgado JD . 2013. Fungemia due to Rhodotorula mucilaginosa in an immunocompetent, critically ill patient. Kansenshogaku Zasshi 87 : 1012.[PubMed]
54. Lopez-Soria LM,, Bereciartua E,, Santamaria M,, Soria LM,, Hernandez-Almaraz JL,, Mularoni A,, Nieto J,, Montejo M . 2013. First case report of catheter-related fungemia by Candida nivariensis in the Iberian Peninsula. Rev Iberoam Micol 30 : 6971.[PubMed]
55. Frange P,, Bougnoux ME,, Lanternier F,, Neven B,, Moshous D,, Angebault C,, Lortholary O,, Blanche S . 2015. An update on pediatric invasive aspergillosis. Med Mal Infect 45 : 189198.[PubMed] [CrossRef]
56. Sole A,, Ussetti P . 2014. Mold infections in lung transplants. Rev Iberoam Micol 31 : 229236.[PubMed]
57. Ruiz-Camps I,, Jarque I . 2014. Invasive mould disease in haematological patients. Rev Iberoam Micol 31 : 249254.[PubMed] [CrossRef]
58. Peman J,, Salavert M . 2014. Invasive fungal disease due to Scedosporium, Fusarium and mucorales. Rev Iberoam Micol 31 : 242248.[PubMed]
59. Pfaller MA,, Messer SA,, Hollis RJ . 1995. Variations in DNA subtype, antifungal susceptibility, and slime production among clinical isolates of Candida parapsilosis . Diagn Microbiol Infect Dis 21 : 914.[PubMed] [CrossRef]
60. Paiva JA,, Pereira JM,, Tabah A,, Mikstacki A,, de Carvalho FB,, Koulenti D,, Ruckly S,, Cakar N,, Misset B,, Dimopoulos G,, Antonelli M,, Rello J,, Ma X,, Tamowicz B,, Timsit JF . 2016. Characteristics and risk factors for 28-day mortality of hospital acquired fungemias in ICUs: data from the EUROBACT study. Crit Care 20 : 53. [PubMed] [CrossRef]
61. Marr KA,, Carter RA,, Boeckh M,, Martin P,, Corey L . 2002. Invasive aspergillosis in allogeneic stem cell transplant recipients: changes in epidemiology and risk factors. Blood 100 : 43584366.[PubMed] [CrossRef]
62. Khoo SH,, Denning DW . 1994. Invasive aspergillosis in patients with AIDS. Clin Infect Dis 19(Suppl 1): S41S48.[PubMed] [CrossRef]
63. Rodriguez-Cerdeira C,, Arenas R,, Moreno-Coutino G,, Vasquez E,, Fernandez R,, Chang P . 2014. Systemic fungal infections in patients with human inmunodeficiency virus. Actas Dermosifiliogr 105 : 517.[PubMed] [CrossRef]
64. Shoham S,, Marr KA . 2012. Invasive fungal infections in solid organ transplant recipients. Future Microbiol 7 : 639655.[PubMed] [CrossRef]
65. Gavalda J,, Meije Y,, Len O,, Pahissa A . 2012. Invasive fungal infection in solid organ transplant. Enferm Infecc Microbiol Clin 30 : 645653.[PubMed] [CrossRef]
66. Junior MC,, de Moraes Arantes A,, Silva HM,, Costa CR,, Silva Mdo R . 2013. Acremonium kiliense: case report and review of published studies. Mycopathologia 176 : 417421.[PubMed] [CrossRef]
67. Garcia-Ruiz JC,, Lopez-Soria L,, Olazabal I,, Amutio E,, Arrieta-Aguirre I,, Velasco-Benito V,, Ponton J,, Moragues MD . 2013. Invasive infections caused by Saprochaete capitata in patients with haematological malignancies: report of five cases and review of the antifungal therapy. Rev Iberoam Micol 30 : 248255.[PubMed] [CrossRef]
68. Rosa C,, Araujo R,, Rodrigues AG,, Pinto-de-Sousa MI,, Pina-Vaz C . 2011. Detection of Aspergillus species in BACTEC blood cultures. J Med Microbiol 60 : 14671471.[PubMed] [CrossRef]
69. Boutati EI,, Anaissie EJ . 1997. Fusarium, a significant emerging pathogen in patients with hematologic malignancy: ten years’ experience at a cancer center and implications for management. Blood 90 : 9991008.[PubMed]
70. Tan TQ,, Ogden AK,, Tillman J,, Demmler GJ,, Rinaldi MG . 1992. Paecilomyceslilacinus catheter-related fungemia in an immunocompromised pediatric patient. J Clin Microbiol 30 : 24792483.[PubMed]
71. Nachman S,, Alpan O,, Malowitz R,, Spitzer ED . 1996. Catheter-associated fungemia due to Wangiella (Exophiala) dermatitidis . J Clin Microbiol 34 : 10111013.[PubMed]
72. Petti CA,, Mirrett S,, Woods CW,, Reller LB . 2005. Controlled clinical comparison of plastic and glass bottles of BacT/ALERT FA medium for culturing organisms from blood of adult patients. J Clin Microbiol 43 : 19601962.[PubMed] [CrossRef]
73. Baron EJ,, Scott JD,, Tompkins LS . 2005. Prolonged incubation and extensive subculturing do not increase recovery of clinically significant microorganisms from standard automated blood cultures. Clin Infect Dis 41 : 16771680.[PubMed] [CrossRef]
74. Doern GV . 2000. Detection of selected fastidious bacteria. Clin Infect Dis 30 : 166173.[PubMed] [CrossRef]
75. Jaeger TM,, Atkinson PP,, Adams BA,, Wright AJ,, Hurt RD . 1988. Legionellabozemanii pneumonia in an immunocompromised patient. Mayo Clin Proc 63 : 7276.[PubMed] [CrossRef]
76. Gallo MT,, Di Domenico EG,, Toma L,, Marchesi F,, Pelagalli L,, Manghisi N,, Ascenzioni F,, Prignano G,, Mengarelli A,, Ensoli F . 2016. Campylobacter jejuni fatal sepsis in a patient with non-Hodgkin’s lymphoma: case report and literature review of a difficult diagnosis. Int J Mol Sci 17 : 544. [PubMed] [CrossRef]
77. van den Bruele T,, Mourad-Baars PE,, Claas EC,, van der Plas RN,, Kuijper EJ,, Bredius RG . 2010. Campylobacter jejuni bacteremia and Helicobacter pylori in a patient with X-linked agammaglobulinemia. Eur J Clin Microbiol Infect Dis 29 : 13151319.[PubMed] [CrossRef]
78. Smith G,, Noon A,, Wolk DM,, Hoover SE . 2012. Helicobacter cinaedi: vertebral osteomyelitis in an immunocompetent individual. Infect Dis Clin Pract. 21 : 6172.
79. Martinez RM,, Shoemaker B,, Riley J,, Wolk DM . 2016. The telegram of the 21st century: the digital Gram stain. ASM Microbe, abstract 322.
80. La Scola B,, Raoult D . 1999. Culture of Bartonella quintana and Bartonella henselae from human samples: a 5-year experience (1993 to 1998). J Clin Microbiol 37 : 18991905.[PubMed]
81. Angus DC,, Linde-Zwirble WT,, Lidicker J,, Clermont G,, Carcillo J,, Pinsky MR . 2001. Epidemiology of severe sepsis in the United States: analysis of incidence, outcome, and associated costs of care. Crit Care Med 29 : 13031310.[PubMed] [CrossRef]
82. Christaki E,, Giamarellos-Bourboulis EJ . 2014. The complex pathogenesis of bacteremia: from antimicrobial clearance mechanisms to the genetic background of the host. Virulence 5 : 5765.[PubMed] [CrossRef]
83. Zhang AQ,, Yue CL,, Gu W,, Du J,, Wang HY,, Jiang J . 2013. Association between CD14 promoter -159C/T polymorphism and the risk of sepsis and mortality: a systematic review and meta-analysis. PLoS One 8 : e71237. [PubMed] [CrossRef]
84. Ouyang L,, Lv YD,, Hou C,, Wu GB,, He ZH . 2013. Quantitative analysis of the association between interleukin-10 1082A/G polymorphism and susceptibility to sepsis. Mol Biol Rep 40 : 43274332.[PubMed] [CrossRef]
85. Ciarlo E,, Savva A,, Roger T . 2013. Epigenetics in sepsis: targeting histone deacetylases. Int J Antimicrob Agents 42(Suppl): S8S12.[PubMed] [CrossRef]
86. O’Hare FM,, William Watson R,, Molloy EJ . 2013. Toll-like receptors in neonatal sepsis. Acta Paediatr 102 : 572578.[PubMed] [CrossRef]
87. Dale AP,, Read RC . 2013. Genetic susceptibility to meningococcal infection. Expert Rev Anti Infect Ther 11 : 187199.[PubMed] [CrossRef]
88. Mayr FB,, Yende S,, Angus DC . 2014. Epidemiology of severe sepsis. Virulence 5 : 411.[PubMed] [CrossRef]
89. Mokart D,, Capo C,, Blache JL,, Delpero JR,, Houvenaeghel G,, Martin C,, Mege JL . 2002. Early postoperative compensatory anti-inflammatory response syndrome is associated with septic complications after major surgical trauma in patients with cancer. Br J Surg 89 : 14501456.[PubMed] [CrossRef]
90. Mokart D,, Leone M,, Sannini A,, Brun JP,, Tison A,, Delpero JR,, Houvenaeghel G,, Blache JL,, Martin C . 2005. Predictive perioperative factors for developing severe sepsis after major surgery. Br J Anaesth 95 : 776781.[PubMed] [CrossRef]
91. Olsen I . 2008. Update on bacteraemia related to dental procedures. Transfus Apher Sci 39 : 173178.[PubMed] [CrossRef]
92. Williams MD,, Braun LA,, Cooper LM,, Johnston J,, Weiss RV,, Qualy RL,, Linde-Zwirble W . 2004. Hospitalized cancer patients with severe sepsis: analysis of incidence, mortality, and associated costs of care. Crit Care 8 : R291R298.[PubMed] [CrossRef]
93. Steinberg JP,, Robichaux C,, Tejedor SC,, Reyes MD,, Jacob JT . 2013. Distribution of pathogens in central line-associated bloodstream infections among patients with and without neutropenia following chemotherapy: evidence for a proposed modification to the current surveillance definition. Infect Control Hosp Epidemiol 34 : 171175.[PubMed] [CrossRef]
94. Todeschini G,, Tecchio C,, Borghero C,, D’Emilio A,, Pegoraro E,, de Lalla F,, Benedetti P,, Spolaore P,, Pellizzer G . 2006. Association between Enterococcus bacteraemia and death in neutropenic patients with haematological malignancies. J Infect 53 : 266273.[PubMed] [CrossRef]
95. Gudiol C,, Bodro M,, Simonetti A,, Tubau F,, Gonzalez-Barca E,, Cisnal M,, Domingo-Domenech E,, Jimenez L,, Carratala J . 2013. Changing aetiology, clinical features, antimicrobial resistance, and outcomes of bloodstream infection in neutropenic cancer patients. Clin Microbiol Infect 19 : 474479.[PubMed] [CrossRef]
96. Dellinger RP,, Levy MM,, Carlet JM,, Bion J,, Parker MM,, Jaeschke R,, Reinhart K,, Angus DC,, Brun-Buisson C,, Beale R,, Calandra T,, Dhainaut JF,, Gerlach H,, Harvey M,, Marini JJ,, Marshall J,, Ranieri M,, Ramsay G,, Sevransky J,, Thompson BT,, Townsend S,, Vender JS,, Zimmerman JL,, Vincent JL . 2008. Surviving Sepsis Campaign: international guidelines for management of severe sepsis and septic shock: 2008. Intensive Care Med 34 : 1760.[PubMed] [CrossRef]
97. Watson RS,, Carcillo JA,, Linde-Zwirble WT,, Clermont G,, Lidicker J,, Angus DC . 2003. The epidemiology of severe sepsis in children in the United States. Am J Respir Crit Care Med 167 : 695701.[PubMed] [CrossRef]
98. Curns AT,, Holman RC,, Sejvar JJ,, Owings MF,, Schonberger LB . 2005. Infectious disease hospitalizations among older adults in the United States from 1990 through 2002. Arch Intern Med 165 : 25142520.[PubMed] [CrossRef]
99. Htwe TH,, Mushtaq A,, Robinson SB,, Rosher RB,, Khardori N . 2007. Infection in the elderly. Infect Dis Clin North Am 21 : 711743ix.[PubMed] [CrossRef]
100. Ortman JM,, Velkoff VA,, Hogan H . 2014. An Aging Nation: The Older Population in the United States. May 24 : 251140. US Census Bureau, Washington DC.
101. Cao Z,, Yende S,, Kellum JA,, Angus DC,, Robinson RA . 2014. Proteomics reveals age-related differences in the host immune response to sepsis 5. J Proteome Res 13 : 422432.[PubMed] [CrossRef]
102. Girard TD,, Opal SM,, Ely EW . 2005. Insights into severe sepsis in older patients: from epidemiology to evidence-based management. Clin Infect Dis 40 : 719727.[PubMed] [CrossRef]
103. Landwehr-Kenzel S,, Henneke P . 2014. Interaction of Streptococcus agalactiae and cellular innate immunity in colonization and disease. Front Immunol 5 : 519. [PubMed] [CrossRef]
104. Chin BS,, Kim MS,, Han SH,, Shin SY,, Choi HK,, Chae YT,, Jin SJ,, Baek JH,, Choi JY,, Song YG,, Kim CO,, Kim JM . 2011. Risk factors of all-cause in-hospital mortality among Korean elderly bacteremic urinary tract infection (UTI) patients. Arch Gerontol Geriatr 52 : e50e55.[PubMed] [CrossRef]
105. Kan B,, Razzaghian HR,, Lavoie PM . 2016. An immunological perspective on neonatal sepsis. Trends Mol Med 22 : 290302.[PubMed] [CrossRef]
106. Hedegaard SS,, Wisborg K,, Hvas AM . 2015. Diagnostic utility of biomarkers for neonatal sepsis—a systematic review. Infect Dis Lond 47 : 117124.[PubMed] [CrossRef]
107. Maheshwari A . 2014. Neutropenia in the newborn. Curr Opin Hematol 21 : 4349.[PubMed] [CrossRef]
108. World Health Organization . 2015. Global Health Observatory Data. http:www.who.int/gho/child_health/mortality/neonate/en. Accessed 14 July 2016.
109. Edwards JR,, Peterson KD,, Mu Y,, Banerjee S,, Allen-Bridson K,, Morrell G,, Dudeck MA,, Pollock DA,, Horan TC . 2009. National Healthcare Safety Network (NHSN) report: data summary for 2006 through 2008, issued December 2009. Am J Infect Control 37 : 783805.[PubMed] [CrossRef]
110. LeBlanc L,, Pepin J,, Toulouse K,, Ouellette MF,, Coulombe MA,, Corriveau MP,, Alary ME . 2006. Fluoroquinolones and risk for methicillin-resistant Staphylococcus aureus, Canada. Emerg Infect Dis 12 : 13981405.[PubMed] [CrossRef]
111. Carratala J,, Alcaide F,, Fernandez-Sevilla A,, Corbella X,, Linares J,, Gudiol F . 1995. Bacteremia due to viridans streptococci that are highly resistant to penicillin: increase among neutropenic patients with cancer. Clin Infect Dis 20 : 11691173.[PubMed] [CrossRef]
112. Rosen MJ,, Narasimhan M . 2006. Critical care of immunocompromised patients: human immunodeficiency virus. Crit Care Med 34 : S245S250.[PubMed] [CrossRef]
113. Huson MA,, Stolp SM,, van der Poll T,, Grobusch MP . 2014. Community-acquired bacterial bloodstream infections in HIV-infected patients: a systematic review. Clin Infect Dis 58 : 7992.[PubMed] [CrossRef]
114. Al-Hasan MN,, Razonable RR,, Eckel-Passow JE,, Baddour LM . 2009. Incidence rate and outcome of Gram-negative bloodstream infection in solid organ transplant recipients. Am J Transplant 9 : 835843.[PubMed] [CrossRef]
115. Linares L,, Garcia-Goez JF,, Cervera C,, Almela M,, Sanclemente G,, Cofan F,, Ricart MJ,, Navasa M,, Moreno A . 2009. Early bacteremia after solid organ transplantation. Transplant Proc 41 : 22622264.[PubMed] [CrossRef]
116. Dorschner P,, McElroy LM,, Ison MG . 2014. Nosocomial infections within the first month of solid organ transplantation. Transpl Infect Dis 16 : 171187.[PubMed] [CrossRef]
117. Candel FJ,, Grima E,, Matesanz M,, Cervera C,, Soto G,, Almela M,, Martinez JA,, Navasa M,, Cofan F,, Ricart MJ,, Perez-Villa F,, Moreno A . 2005. Bacteremia and septic shock after solid-organ transplantation. Transplant Proc 37 : 40974099.[PubMed] [CrossRef]
118. Kawecki D,, Chmura A,, Pacholczyk M,, Lagiewska B,, Adadynski L,, Wasiak D,, Malkowski P,, Rokosz A,, Sawicka-Grzelak A,, Szymanowska A,, Swoboda-Kopec E,, Wroblewska M,, Rowinski W,, Durlik M,, Luczak M . 2007. Etiological agents of bacteremia in the early period after liver transplantation. Transplant Proc 39 : 28162821.[PubMed] [CrossRef]
119. Moreno A,, Cervera C,, Gavalda J,, Rovira M,, de la Camara R,, Jarque I,, Montejo M,, de la Torre-Cisneros J,, Miguel Cisneros J,, Fortun J,, Lopez-Medrano F,, Gurgui M,, Munoz P,, Ramos A,, Carratala J . 2007. Bloodstream infections among transplant recipients: results of a nationwide surveillance in Spain. Am J Transplant 7 : 25792586.[PubMed] [CrossRef]
120. Annane D,, Bellissant E,, Bollaert PE,, Briegel J,, Keh D,, Kupfer Y . 2004. Corticosteroids for treating severe sepsis and septic shock. Cochrane Database Syst Rev 1. [CrossRef]
121. Marik PE . 2011. Glucocorticoids in sepsis: dissecting facts from fiction. Crit Care 15 : 158. [PubMed] [CrossRef]
122. Sanchez Porto A,, Casas Ciria J,, Roman Enri M,, Garcia Collado S,, Bachiller Luque MR,, Eiros JM . 2014. Leclercia adecarboxylata bacteraemia in an immunocompromised patient with metabolic syndrome. Infez Med 22 : 149151.[PubMed]
123. Llorente A,, Perez-Valero I,, Garcia E,, Heras I,, Fraile V,, Garcia P,, Lopez J,, Salavert M,, Bobillo F . 2011. Mortality risk factors in patients with zygomycosis: a retrospective and multicentre study of 25 cases. Enferm Infecc Microbiol Clin 29 : 263268.[PubMed] [CrossRef]
124. Mesejo A,, Sanchez Alvarez C,, Arboleda Sanchez JA , Metabolism and Nutrition Working Group of the Spanish Society of Intensive Care Medicine and Coronary Units . 2011. Guidelines for specialized nutritional and metabolic support in the critically ill patient: update. Consensus SEMICYUC-SENPE: obese patient. Nutr Hosp 26(Suppl 2): 5458.[PubMed]
125. Berger MM,, Oudemans-van Straaten HM . 2015. Vitamin C supplementation in the critically ill patient. Curr Opin Clin Nutr Metab Care 18 : 193201.[PubMed] [CrossRef]
126. Badurdeen S,, Mulongo M,, Berkley JA . 2015. Arginine depletion increases susceptibility to serious infections in preterm newborns. Pediatr Res 77 : 290297.[PubMed]
127. Upala S,, Sanguankeo A,, Permpalung N . 2015. Significant association between vitamin D deficiency and sepsis: a systematic review and meta-analysis. BMC Anesthesiol 15 : 84. [PubMed] [CrossRef]
128. de Haan K,, Groeneveld AB,, de Geus HR,, Egal M,, Struijs A . 2014. Vitamin D deficiency as a risk factor for infection, sepsis and mortality in the critically ill: systematic review and meta-analysis. Crit Care 18 : 660. [PubMed] [CrossRef]
129. Huang Z,, Rose AH,, Hoffmann PR . 2012. The role of selenium in inflammation and immunity: from molecular mechanisms to therapeutic opportunities. Antioxid Redox Signal 16 : 705743.[PubMed] [CrossRef]
130. Ishida JH,, Johansen KL . 2014. Iron and infection in hemodialysis patients. Semin Dial 27 : 2636.[PubMed] [CrossRef]
131. Manzanares W,, Hardy G . 2011. Thiamine supplementation in the critically ill. Curr Opin Clin Nutr Metab Care 14 : 610617.[PubMed] [CrossRef]
132. Lang D,, Mead JS,, Sykes DB . 2015. Hormones and the bone marrow: panhypopituitarism and pancytopenia in a man with a pituitary adenoma. J Gen Intern Med 30 : 692696.[PubMed] [CrossRef]
133. Sfera A,, Cummings M,, Osorio C . 2015. Non-neuronal acetylcholine: the missing link between sepsis, cancer, and delirium? Front Med Lausanne 2 : 56. [CrossRef]
134. Soeters PB,, Grecu I . 2012. Have we enough glutamine and how does it work? A clinician’s view. Ann Nutr Metab 60 : 1726.[PubMed] [CrossRef]
135. Velissaris D,, Karamouzos V,, Pierrakos C,, Aretha D,, Karanikolas M . 2015. Hypomagnesemia in critically ill sepsis patients. J Clin Med Res 7 : 911918.[PubMed] [CrossRef]
136. Wijnands KA,, Castermans TM,, Hommen MP,, Meesters DM,, Poeze M . 2015. Arginine and citrulline and the immune response in sepsis. Nutrients 7 : 14261463.[PubMed] [CrossRef]
137. John M. Eisenberg Center for Clinical Decisions and Communication Science . 2012. Procalcitonin-Guided Antibiotic Therapy. Comparative Effectiveness Review Summary Guides for Clinicians, Rockville, MD.
138. National Collaborating Centre for Cancer . 2012. Neutropenic Sepsis: Prevention and Management of Neutropenic Sepsis in Cancer Patients, National Institute for Health and Clinical Excellence Guidance, London.
139. Barton CD,, Waugh LK,, Nielsen MJ,, Paulus S . 2015. Febrile neutropenia in children treated for malignancy. J Infect 71(Suppl 1): S27S35.[PubMed] [CrossRef]
140. Bow EJ . 2013. Infection in neutropenic patients with cancer. Crit Care Clin 29 : 411441.[PubMed] [CrossRef]
141. Ahn S,, Lee YS . 2012. Predictive factors for poor prognosis febrile neutropenia. Curr Opin Oncol 24 : 376380.[PubMed] [CrossRef]
142. Clarke RT,, Warnick J,, Stretton K,, Littlewood TJ . 2011. Improving the immediate management of neutropenic sepsis in the UK: lessons from a national audit. Br J Haematol 153 : 773779.[PubMed] [CrossRef]
143. Gafter-Gvili A,, Fraser A,, Paul M,, Vidal L,, Lawrie TA,, van de Wetering MD,, Kremer LC,, Leibovici L . 2012. Antibiotic prophylaxis for bacterial infections in afebrile neutropenic patients following chemotherapy. Cochrane Database Syst Rev 1 : CD004386. [PubMed] [CrossRef]
144. Lin SG,, Hou TY,, Huang DH,, He SY,, Lin YD,, Zhang LY,, Hsieh PS . 2012. Role of procalcitonin in the diagnosis of severe infection in pediatric patients with fever and neutropenia—a systemic review and meta-analysis. Pediatr Infect Dis J 31 : e182e188.[PubMed] [CrossRef]
145. Rolston KV . 2014. Neutropenic fever and sepsis: evaluation and management. Cancer Treat Res 161 : 181202.[PubMed] [CrossRef]
146. White L,, Ybarra M . 2014. Neutropenic fever. Emerg Med Clin North Am 32 : 549561.[PubMed] [CrossRef]
147. Thursky KA,, Worth LJ . 2015. Can mortality of cancer patients with fever and neutropenia be improved? Curr Opin Infect Dis 28 : 505513.[PubMed] [CrossRef]
148. Hsu WY,, Chiou SS,, Liao YM,, Shu HL,, Zeng YS,, Wong CC,, Lin PC . 2016. Successful management of multilineage autoimmune cytopenia complicated with severe infection and deep vein thrombosis in a patient with Hodgkin lymphoma post-autologous hematopoietic stem cell transplantation. Pediatr Transplant 20 : 168171.[PubMed] [CrossRef]
149. Kamei K,, Takahashi M,, Fuyama M,, Saida K,, Machida H,, Sato M,, Ogura M,, Ito S . 2015. Rituximab-associated agranulocytosis in children with refractory idiopathic nephrotic syndrome: case series and review of literature. Nephrol Dial Transplant 30 : 9196.[PubMed] [CrossRef]
150. Nittala S,, Subbarao GC,, Maheshwari A . 2012. Evaluation of neutropenia and neutrophilia in preterm infants. J Matern Fetal Neonatal Med 25 : 100103.[PubMed] [CrossRef]
151. Pammi M,, Brocklehurst P . 2011. Granulocyte transfusions for neonates with confirmed or suspected sepsis and neutropenia. Cochrane Database Syst Rev doi:10.1002/14651858.CD003956. [PubMed] [CrossRef]
152. Silvestris N,, Del Re M,, Azzariti A,, Maiello E,, Lombardi L,, Cinieri S,, Guarini A,, Brunetti AE,, Delcuratolo S,, De Vita F,, Pisconti S,, Danesi R,, Colucci G . 2012. Optimized granulocyte colony-stimulating factor prophylaxis in adult cancer patients: from biological principles to clinical guidelines. Expert Opin Ther Targets 16(Suppl 2): S111S117.[PubMed] [CrossRef]
153. Welsh SJ,, Strauss SJ . 2012. Management of the patient with neutropenic sepsis. Br J Hosp Med (Lond) 73 : C101C103.[CrossRef]
154. Singer M,, Deutschman CS,, Seymour CW,, Shankar-Hari M,, Annane D,, Bauer M,, Bellomo R,, Bernard GR,, Chiche JD,, Coopersmith CM,, Hotchkiss RS,, Levy MM,, Marshall JC,, Martin GS,, Opal SM,, Rubenfeld GD,, van der Poll T,, Vincent JL,, Angus DC . 2016. The third international consensus definitions for sepsis and septic shock (sepsis-3). JAMA 315 : 801810.[PubMed] [CrossRef]
155. Shankar-Hari M,, Phillips GS,, Levy ML,, Seymour CW,, Liu VX,, Deutschman CS,, Angus DC,, Rubenfeld GD,, Singer M , Sepsis Definitions Task Force . 2016. Developing a new definition and assessing new clinical criteria for septic shock: for the third international consensus definitions for sepsis and septic shock (sepsis-3). JAMA 315 : 775787.[PubMed] [CrossRef]
156. Keynan Y,, Rubinstein E . 2013. Staphylococcus aureus bacteremia, risk factors, complications, and management. Crit Care Clin 29 : 547562.[PubMed] [CrossRef]
157. Ramanan P,, Deziel PJ,, Wengenack NL . 2013. Gordonia bacteremia. J Clin Microbiol 51 : 34433447.[PubMed] [CrossRef]
158. Ramanan P,, Razonable RR . 2013. Elizabethkingia species sepsis after lung transplantation: case report and literature review. Transpl Infect Dis 15 : E229E234.[PubMed] [CrossRef]
159. Myers KA,, Jeffery RM,, Lodha A . 2012. Late-onset Leclercia adecarboxylata bacteraemia in a premature infant in the NICU. Acta Paediatr 101 : e37e39.[PubMed] [CrossRef]
160. Garazi M,, Singer C,, Tai J,, Ginocchio CC . 2012. Bloodstream infections caused by Stenotrophomonas maltophilia: a seven-year review. J Hosp Infect 81 : 114118.[PubMed] [CrossRef]
161. Monneret G,, Venet F,, Kullberg BJ,, Netea MG . 2011. ICU-acquired immunosuppression and the risk for secondary fungal infections. Med Mycol 49(Suppl 1): S17S23.[PubMed] [CrossRef]
162. Haeusler GM,, Levene I . 2015. Question 2: what are the risk factors for antibiotic resistant Gram-negative bacteraemia in children with cancer? Arch Dis Child 100 : 895898.[PubMed] [CrossRef]
163. Mori M,, Tsunemine H,, Imada K,, Ito K,, Kodaka T,, Takahashi T . 2014. Life-threatening hemorrhagic pneumonia caused by Stenotrophomonas maltophilia in the treatment of hematologic diseases. Ann Hematol 93 : 901911.[PubMed] [CrossRef]
164. Bassetti M,, Righi E . 2013. Multidrug-resistant bacteria: what is the threat? Hematology (Am Soc Hematol Educ Program) 2013 : 428432.[PubMed] [CrossRef]
165. Lode HM,, Stahlmann R,, Kresken M . 2013. Multiresistant pathogens—a challenge for clinicians. Zentralbl Chir 138 : 549553.[PubMed]
166. Mellata M . 2013. Human and avian extraintestinal pathogenic Escherichia coli: infections, zoonotic risks, and antibiotic resistance trends. Foodborne Pathog Dis 10 : 916932.[PubMed] [CrossRef]
167. Baylan O . 2012. An opportunistic pathogen frequently isolated from immunocompromised patients: Burkholderia cepacia complex. Mikrobiyol Bul 46 : 304318.[PubMed]
168. Salimnia H,, Alangaden GJ,, Bharadwaj R,, Painter TM,, Chandrasekar PH,, Fairfax MR . 2011. Weissella confusa: an unexpected cause of vancomycin-resistant gram-positive bacteremia in immunocompromised hosts. Transpl Infect Dis 13 : 294298.[PubMed] [CrossRef]
169. Hunfeld KP,, Bingold T,, Brade V,, Wissing H . 2008. Molecular biological detection of pathogens in patients with sepsis. Potentials, limitations and perspectives. Anaesthesist 57 : 326337.[PubMed] [CrossRef]
170. Hayden RT,, Isotalo PA,, Parrett T,, Wolk DM,, Qian X,, Roberts GD,, Lloyd RV . 2003. In situ hybridization for the differentiation of Aspergillus, Fusarium, and Pseudallescheria species in tissue section. Diagn Mol Pathol 12 : 2126.[PubMed] [CrossRef]
171. Leber A (ed). 2016. Clinical Microbiology Procedures Manual. ASM Press, Washington, D.C.
172. Caliendo AM,, Gilbert DN,, Ginocchio CC,, Hanson KE,, May L,, Quinn TC,, Tenover FC,, Alland D,, Blaschke AJ,, Bonomo RA,, Carroll KC,, Ferraro MJ,, Hirschhorn LR,, Joseph WP,, Karchmer T,, MacIntyre AT,, Reller LB,, Jackson AF . 2013. Better tests, better care: improved diagnostics for infectious diseases. Clin Infect Dis 57(Suppl 3): S139S170.[PubMed] [CrossRef]
173. Barenfanger J,, Graham DR,, Kolluri L,, Sangwan G,, Lawhorn J,, Drake CA,, Verhulst SJ,, Peterson R,, Moja LB,, Ertmoed MM,, Moja AB,, Shevlin DW,, Vautrain R,, Callahan CD . 2008. Decreased mortality associated with prompt Gram staining of blood cultures. Am J Clin Pathol 130 : 870876.[PubMed] [CrossRef]
174. Fenollar F,, Raoult D . 2007. Molecular diagnosis of bloodstream infections caused by non-cultivable bacteria. Int J Antimicrob Agents 30(Suppl 1): S7S15.[PubMed] [CrossRef]
175. Kumar A,, Haery C,, Paladugu B,, Kumar A,, Symeoneides S,, Taiberg L,, Osman J,, Trenholme G,, Opal SM,, Goldfarb R,, Parrillo JE . 2006. The duration of hypotension before the initiation of antibiotic treatment is a critical determinant of survival in a murine model of Escherichia coli septic shock: association with serum lactate and inflammatory cytokine levels. J Infect Dis 193 : 251258.[PubMed] [CrossRef]
176. Diab M,, El-Damarawy M,, Shemis M . 2008. Rapid identification of methicillin-resistant staphylococci bacteremia among intensive care unit patients. Medscape J Med 10 : 126. [PubMed]
177. Sturm PD,, Kwa D,, Vos FJ,, Bartels CJ,, Schulin T . 2008. Performance of two tube coagulase methods for rapid identification of Staphylococcus aureus from blood cultures and their impact on antimicrobial management. Clin Microbiol Infect 14 : 510513.[PubMed] [CrossRef]
178. Bennett K,, Sharp SE . 2008. Rapid differentiation of methicillin-resistant Staphylococcus aureus and methicillin-susceptible Staphylococcus aureus from blood cultures by use of a direct cefoxitin disk diffusion test. J Clin Microbiol 46 : 38363838.[PubMed] [CrossRef]
179. Qian Q,, Eichelberger K,, Kirby JE . 2007. Rapid identification of Staphylococcusaureus in blood cultures by use of the direct tube coagulase test. J Clin Microbiol 45 : 22672269.[PubMed] [CrossRef]
180. Marlowe EM,, Linscott AJ,, Kanatani M,, Bruckner DA . 2002. Practical therapeutic application of the oxoid PBP2′ latex agglutination test for the rapid identification of methicillin-resistant Staphylococcus aureus in blood cultures. Am J Clin Pathol 118 : 287291.[PubMed] [CrossRef]
181. Kristensen B,, Hojbjerg T,, Schonheyder HC . 2001. Rapid immunodiagnosis of streptococci and enterococci in blood cultures. APMIS 109 : 284288.[PubMed] [CrossRef]
182. Terlecka JA,, du Cros PA,, Orla MC,, Spelman D . 2007. Rapid differentiation of Candida albicans from non-albicans species by germ tube test directly from BacTAlert blood culture bottles. Mycoses 50 : 4851.[PubMed] [CrossRef]
183. Harriau P,, Ruffel F,, Lardy JB . 2006. Use of BioRad plating agar MRSASelect for the daily detection of methicillin resistant staphylococci isolated from samples taken from blood culture bottles. Pathol Biol (Paris) 54 : 506509.[PubMed] [CrossRef]
184. Chan WW,, Lovgren M,, Tyrrell GJ . 2009. Utility of the pneumoslide test in identification of Streptococcus pneumoniae in blood cultures and its relation to capsular serotype. J Clin Microbiol 47 : 15591561.[PubMed] [CrossRef]
185. Chung JW,, Jeon HS,, Sung H,, Kim MN . 2009. Evaluation of MicroScan and Phoenix system for rapid identification and susceptibility testing using direct inoculation from positive BACTEC blood culture bottles. Korean J Lab Med 29 : 2534.[PubMed] [CrossRef]
186. Chen JR,, Lee SY,, Yang BH,, Lu JJ . 2008. Rapid identification and susceptibility testing using the VITEK 2 system using culture fluids from positive BacT/ALERT blood cultures. J Microbiol Immunol Infect 41 : 259264.[PubMed]
187. Avolio M,, Grosso S,, Bruschetta G,, De RR,, Camporese A . 2009. Direct antifungal susceptibility testing of positive Candida blood cultures by sensititre YeastOne. New Microbiol 32 : 179184.[PubMed]
188. Reimer LG,, Wilson ML,, Weinstein MP . 1997. Update on detection of bacteremia and fungemia. Clin Microbiol Rev 10 : 444465.[PubMed]
189. Flayhart D,, Borek AP,, Wakefield T,, Dick J,, Carroll KC . 2007. Comparison of BACTEC PLUS blood culture media to BacT/Alert FA blood culture media for detection of bacterial pathogens in samples containing therapeutic levels of antibiotics. J Clin Microbiol 45 : 816821.[PubMed] [CrossRef]
190. Cantey JB,, Milstone AM . 2015. Bloodstream infections: epidemiology and resistance. Clin Perinatol 42 : 116.[PubMed] [CrossRef]
191. Weinstein MP,, Towns ML,, Quartey SM,, Mirrett S,, Reimer LG,, Parmigiani G,, Reller LB . 1997. The clinical significance of positive blood cultures in the 1990s: a prospective comprehensive evaluation of the microbiology, epidemiology, and outcome of bacteremia and fungemia in adults. Clin Infect Dis 24 : 584602.[PubMed] [CrossRef]
192. Weinstein MP . 2003. Blood culture contamination: persisting problems and partial progress. J Clin Microbiol 41 : 22752278.[PubMed] [CrossRef]