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Chapter 12 : The Vaginal Microbiome

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The Vaginal Microbiome, Page 1 of 2

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Abstract:

The collection of microbes (microbiota) in the human vagina with their associated genes (microbiome) and gene products helps to shape the physiology of the vagina (1). Different vaginal microbial communities are associated with different metabolic states, immunological responses, and risks of adverse health outcomes including preterm birth and acquisition of sexually transmitted infections. Tools in molecular microbiology have cataloged the diversity of vaginal microbes, leading to a more complete census, and have identified species that may be useful indicators of health or markers of disease risk.

Citation: Fredricks D. 2016. The Vaginal Microbiome, p 138-145. In Persing D, Tenover F, Hayden R, Ieven M, Miller M, Nolte F, Tang Y, van Belkum A (ed), Molecular Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555819071.ch12
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Image of FIGURE 1
FIGURE 1

Vaginal bacterial communities in all women. (A) A novel variant of hierarchical clustering was used to generate a clustering tree depicting the bacterial diversity in 220 women with and without BV. The scale bar represents KR distance, a generalization of UniFrac. The colored bars represent bacterial taxa that were most abundant in each sample. Less abundant taxa are grouped in the “other” category. BV status by Amsel criteria (B) and Gram stain (C) is provided for all women in the two vertical bars. In the absence of BV, the vaginal microbiota was dominated by or . Women with BV had more diverse bacterial communities ( ).

Citation: Fredricks D. 2016. The Vaginal Microbiome, p 138-145. In Persing D, Tenover F, Hayden R, Ieven M, Miller M, Nolte F, Tang Y, van Belkum A (ed), Molecular Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555819071.ch12
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1. O'Hanlon DE, Moench TR, Cone RA. 2013. Vaginal pH and microbicidal lactic acid when lactobacilli dominate the microbiota. PLoS One 8:e80074[CrossRef].[PubMed]
2. Huttenhower C, , et al, Human Microbiome Project Consortium. 2012. Structure, function and diversity of the healthy human microbiome. Nature 486:207214[CrossRef].[PubMed]
3. Srinivasan S, Liu C, Mitchell CM, Fiedler TL, Thomas KK, Agnew KJ, Marrazzo JM, Fredricks DN. 2010. Temporal variability of human vaginal bacteria and relationship with bacterial vaginosis. PLoS One 5:e10197[CrossRef].[PubMed]
4. Hummelen R, Macklaim JM, Bisanz JE, Hammond JA, McMillan A, Vongsa R, Koenig D, Gloor GB, Reid G. 2011. Vaginal microbiome and epithelial gene array in post-menopausal women with moderate to severe dryness. PLoS One 6:e26602[CrossRef].[PubMed]
5. Moore DE, Soules MR, Klein NA, Fujimoto VY, Agnew KJ, Eschenbach DA. 2000. Bacteria in the transfer catheter tip influence the live-birth rate after in vitro fertilization. Fertil Steril 74:11181124[CrossRef].[PubMed]
6. Dominguez-Bello MG, Costello EK, Contreras M, Magris M, Hidalgo G, Fierer N, Knight R. 2010. Delivery mode shapes the acquisition and structure of the initial microbiota across multiple body habitats in newborns. Proc Natl Acad Sci USA 107:1197111975[CrossRef].[PubMed]
7. Leitich H, Bodner-Adler B, Brunbauer M, Kaider A, Egarter C, Husslein P. 2003. Bacterial vaginosis as a risk factor for preterm delivery: a meta-analysis. Am J Obstet Gynecol 189:139147[CrossRef].[PubMed]
8. DiGiulio DB, Romero R, Kusanovic JP, Gómez R, Kim CJ, Seok KS, Gotsch F, Mazaki-Tovi S, Vaisbuch E, Sanders K, Bik EM, Chaiworapongsa T, Oyarzún E, Relman DA. 2010. Prevalence and diversity of microbes in the amniotic fluid, the fetal inflammatory response, and pregnancy outcome in women with preterm pre-labor rupture of membranes. Am J Reprod Immunol 64:3857.[PubMed]
9. Joergensen JS, Kjær Weile LK, Lamont RF. 2014. The early use of appropriate prophylactic antibiotics in susceptible women for the prevention of preterm birth of infectious etiology. Expert Opin Pharmacother 15:21732191[CrossRef].[PubMed]
10. Lamont RF, Nhan-Chang CL, Sobel JD, Workowski K, Conde-Agudelo A, Romero R. 2011. Treatment of abnormal vaginal flora in early pregnancy with clindamycin for the prevention of spontaneous preterm birth: a systematic review and metaanalysis. Am J Obstet Gynecol 205:177190[CrossRef].[PubMed]
11. McDonald H, Brocklehurst P, Parsons J. 2005. Antibiotics for treating bacterial vaginosis in pregnancy. Cochrane Database Syst Rev 1:CD000262.[PubMed]
12. Sobel JD. 2000. Bacterial vaginosis. Annu Rev Med 51:349356[CrossRef].[PubMed]
13. Sobel JD. 2005. What's new in bacterial vaginosis and trichomoniasis? Infect Dis Clin North Am 19:387406[CrossRef].[PubMed]
14. Cohen CR, Lingappa JR, Baeten JM, Ngayo MO, Spiegel CA, Hong T, Donnell D, Celum C, Kapiga S, Delany S, Bukusi EA. 2012. Bacterial vaginosis associated with increased risk of female-to-male HIV-1 transmission: a prospective cohort analysis among African couples. PLoS Med 9:e1001251[CrossRef].[PubMed]
15. Ravel J, Gajer P, Abdo Z, Schneider GM, Koenig SS, McCulle SL, Karlebach S, Gorle R, Russell J, Tacket CO, Brotman RM, Davis CC, Ault K, Peralta L, Forney LJ. 2011. Vaginal microbiome of reproductive-age women. Proc Natl Acad Sci USA 108(Suppl 1):46804687[CrossRef].[PubMed]
16. Srinivasan S, Hoffman NG, Morgan MT, Matsen FA, Fiedler TL, Hall RW, Ross FJ, McCoy CO, Bumgarner R, Marrazzo JM, Fredricks DN. 2012. Bacterial communities in women with bacterial vaginosis: high resolution phylogenetic analyses reveal relationships of microbiota to clinical criteria. PLoS One 7:e37818[CrossRef].[PubMed]
17. Smith BC, McAndrew T, Chen Z, Harari A, Barris DM, Viswanathan S, Rodriguez AC, Castle P, Herrero R, Schiffman M, Burk RD. 2012. The cervical microbiome over 7 years and a comparison of methodologies for its characterization. PLoS One 7:e40425[CrossRef].[PubMed]
18. Frank JA, Reich CI, Sharma S, Weisbaum JS, Wilson BA, Olsen GJ. 2008. Critical evaluation of two primers commonly used for amplification of bacterial 16S rRNA genes. Appl Environ Microbiol 74:24612470[CrossRef].[PubMed]
19. Borgdorff H, Tsivtsivadze E, Verhelst R, Marzorati M, Jurriaans S, Ndayisaba GF, Schuren FH, van de Wijgert JH. 2014. Lactobacillus-dominated cervicovaginal microbiota associated with reduced HIV/STI prevalence and genital HIV viral load in African women. ISME J 8:17811793[CrossRef].[PubMed]
20. Ness RB, Kip KE, Hillier SL, Soper DE, Stamm CA, Sweet RL, Rice P, Richter HE. 2005. A cluster analysis of bacterial vaginosis-associated microflora and pelvic inflammatory disease. Am J Epidemiol 162:585590[CrossRef].[PubMed]
21. Wilks M, Wiggins R, Whiley A, Hennessy E, Warwick S, Porter H, Corfield A, Millar M. 2004. Identification and H(2)O(2) production of vaginal lactobacilli from pregnant women at high risk of preterm birth and relation with outcome. J Clin Microbiol 42:713717[CrossRef].[PubMed]
22. Mirmonsef P, Gilbert D, Veazey RS, Wang J, Kendrick SR, Spear GT. 2012. A comparison of lower genital tract glycogen and lactic acid levels in women and macaques: implications for HIV and SIV susceptibility. AIDS Res Hum Retroviruses 28:7681[CrossRef].[PubMed]
23. Atassi F, Servin AL. 2010. Individual and co-operative roles of lactic acid and hydrogen peroxide in the killing activity of enteric strain Lactobacillus johnsonii NCC933 and vaginal strain Lactobacillus gasseri KS120.1 against enteric, uropathogenic and vaginosis-associated pathogens. FEMS Microbiol Lett 304:2938[CrossRef].[PubMed]
24. Stoyancheva G, Marzotto M, Dellaglio F, Torriani S. 2014. Bacteriocin production and gene sequencing analysis from vaginal Lactobacillus strains. Arch Microbiol 196:645653[CrossRef].[PubMed]
25. Gajer P, Brotman RM, Bai G, Sakamoto J, Schütte UM, Zhong X, Koenig SS, Fu L, Ma ZS, Zhou X, Abdo Z, Forney LJ, Ravel J. 2012. Temporal dynamics of the human vaginal microbiota. Sci Transl Med 4:132ra52[CrossRef].[PubMed]
26. Schwebke JR. 2000. Asymptomatic bacterial vaginosis: response to therapy. Am J Obstet Gynecol 183:14341439[CrossRef].[PubMed]
27. Allsworth JE, Peipert JF. 2007. Prevalence of bacterial vaginosis: 2001–2004 National Health and Nutrition Examination Survey data. Obstet Gynecol 109:114120[CrossRef].[PubMed]
28. Sobel JD. 2002. Treatment of vaginal Candida infections. Expert Opin Pharmacother 3:10591065[CrossRef].[PubMed]
29. Sobel JD, Vazquez J, Lynch M, Meriwether C, Zervos MJ. 1993. Vaginitis due to Saccharomyces cerevisiae: epidemiology, clinical aspects, and therapy. Clin Infect Dis 16:9399[CrossRef].[PubMed]
30. Fidel PL Jr, Sobel JD. 1996. Immunopathogenesis of recurrent vulvovaginal candidiasis. Clin Microbiol Rev 9:335348.[PubMed]
31. Kiliç AO, Pavlova SI, Alpay S, Kiliç SS, Tao L. 2001. Comparative study of vaginal Lactobacillus phages isolated from women in the United States and Turkey: prevalence, morphology, host range, and DNA homology. Clin Diagn Lab Immunol 8:3139.[PubMed]
32. Nelson DB, Bellamy S, Odibo A, Nachamkin I, Ness RB, Allen-Taylor L. 2007. Vaginal symptoms and bacterial vaginosis (BV): how useful is self-report? Development of a screening tool for predicting BV status. Epidemiol Infect 135:13691375[CrossRef].[PubMed]
33. Amsel R, Totten PA, Spiegel CA, Chen KC, Eschenbach D, Holmes KK. 1983. Nonspecific vaginitis. Diagnostic criteria and microbial and epidemiologic associations. Am J Med 74:1422[CrossRef].[PubMed]
34. Nugent RP, Krohn MA, Hillier SL. 1991. Reliability of diagnosing bacterial vaginosis is improved by a standardized method of gram stain interpretation. J Clin Microbiol 29:297301.[PubMed]
35. Ison CA, Hay PE. 2002. Validation of a simplified grading of Gram stained vaginal smears for use in genitourinary medicine clinics. Sex Transm Infect 78:413415[CrossRef].[PubMed]
36. Carey JC, Klebanoff MA, Hauth JC, Hillier SL, Thom EA, Ernest JM, Heine RP, Nugent RP, Fischer ML, Leveno KJ, Wapner R, Varner M, Trout W, Moawad A, Sibai BM, Miodovnik M, Dombrowski M, O'Sullivan MJ, VanDorsten JP, Langer O, Roberts J, National Institute of Child Health and Human Development Network of Maternal-Fetal Medicine Units. 2000. Metronidazole to prevent preterm delivery in pregnant women with asymptomatic bacterial vaginosis. N Engl J Med 342:534540[CrossRef].[PubMed]
37. McDonald HM, O'Loughlin JA, Vigneswaran R, Jolley PT, Harvey JA, Bof A, McDonald PJ. 1997. Impact of metronidazole therapy on preterm birth in women with bacterial vaginosis flora (Gardnerella vaginalis): a randomised, placebo controlled trial. Br J Obstet Gynaecol 104:13911397[CrossRef].[PubMed]
38. Larsson PG, Fåhraeus L, Carlsson B, Jakobsson T, Forsum U, Premature study group of the Southeast Health Care Region of Sweden. 2006. Late miscarriage and preterm birth after treatment with clindamycin: a randomised consent design study according to Zelen. BJOG 113:629637[CrossRef].[PubMed]
39. Spiegel CA. 1991. Bacterial vaginosis. Clin Microbiol Rev 4:485502.[PubMed]
40. Gardner HL, Dukes CD. 1955. Haemophilus vaginalis vaginitis: a newly defined specific infection previously classified non-specific vaginitis. Am J Obstet Gynecol 69:962976.[PubMed]
41. Fredricks DN, Fiedler TL, Thomas KK, Oakley BB, Marrazzo JM. 2007. Targeted PCR for detection of vaginal bacteria associated with bacterial vaginosis. J Clin Microbiol 45:32703276[CrossRef].[PubMed]
42. Menard JP, Fenollar F, Henry M, Bretelle F, Raoult D. 2008. Molecular quantification of Gardnerella vaginalis and Atopobium vaginae loads to predict bacterial vaginosis. Clin Infect Dis 47:3343[CrossRef].[PubMed]
43. Swidsinski A, Mendling W, Loening-Baucke V, Ladhoff A, Swidsinski S, Hale LP, Lochs H. 2005. Adherent biofilms in bacterial vaginosis. Obstet Gynecol 106:10131023[CrossRef].[PubMed]
44. Swidsinski A, Mendling W, Loening-Baucke V, Swidsinski S, Dörffel Y, Scholze J, Lochs H, Verstraelen H. 2008. An adherent Gardnerella vaginalis biofilm persists on the vaginal epithelium after standard therapy with oral metronidazole. Am J Obstet Gynecol 198:97.e197.e6[CrossRef].[PubMed]
45. Verhelst R, Verstraelen H, Claeys G, Verschraegen G, Delanghe J, Van Simaey L, De Ganck C, Temmerman M, Vaneechoutte M. 2004. Cloning of 16S rRNA genes amplified from normal and disturbed vaginal microflora suggests a strong association between Atopobium vaginae, Gardnerella vaginalis and bacterial vaginosis. BMC Microbiol 4:16[CrossRef].[PubMed]
46. Fredricks DN, Fiedler TL, Marrazzo JM. 2005. Molecular identification of bacteria associated with bacterial vaginosis. N Engl J Med 353:18991911[CrossRef].[PubMed]
47. Schellenberg JJ, Links MG, Hill JE, Dumonceaux TJ, Kimani J, Jaoko W, Wachihi C, Mungai JN, Peters GA, Tyler S, Graham M, Severini A, Fowke KR, Ball TB, Plummer FA. 2011. Molecular definition of vaginal microbiota in East African commercial sex workers. Appl Environ Microbiol 77:40664074[CrossRef].[PubMed]
48. Schellenberg J, Links MG, Hill JE, Dumonceaux TJ, Peters GA, Tyler S, Ball TB, Severini A, Plummer FA. 2009. Pyrosequencing of the chaperonin-60 universal target as a tool for determining microbial community composition. Appl Environ Microbiol 75:28892898[CrossRef].[PubMed]
49. Srinivasan S, Fredricks DN. 2008. The human vaginal bacterial biota and bacterial vaginosis. Interdiscip Perspect Infect Dis 2008:750479[CrossRef].[PubMed]
50. Bradshaw CS, Tabrizi SN, Fairley CK, Morton AN, Rudland E, Garland SM. 2006. The association of Atopobium vaginae and Gardnerella vaginalis with bacterial vaginosis and recurrence after oral metronidazole therapy. J Infect Dis 194:828836[CrossRef].[PubMed]
51. Fredricks DN, Fiedler TL, Thomas KK, Mitchell CM, Marrazzo JM. 2009. Changes in vaginal bacterial concentrations with intravaginal metronidazole therapy for bacterial vaginosis as assessed by quantitative PCR. J Clin Microbiol 47:721726[CrossRef].[PubMed]
52. Gazi H, Degerli K, Kurt O, Teker A, Uyar Y, Caglar H, Kurutepe S, Surucuoglu S. 2006. Use of DNA hybridization test for diagnosing bacterial vaginosis in women with symptoms suggestive of infection. APMIS 114:784787[CrossRef].[PubMed]
53. Brown HL, Fuller DD, Jasper LT, Davis TE, Wright JD. 2004. Clinical evaluation of affirm VPIII in the detection and identification of Trichomonas vaginalis, Gardnerella vaginalis, and Candida species in vaginitis/vaginosis. Infect Dis Obstet Gynecol 12:1721[CrossRef].[PubMed]
54. Witt A, Petricevic L, Kaufmann U, Gregor H, Kiss H. 2002. DNA hybridization test: rapid diagnostic tool for excluding bacterial vaginosis in pregnant women with symptoms suggestive of infection. J Clin Microbiol 40:30573059[CrossRef].[PubMed]
55. Bradshaw CS, Morton AN, Garland SM, Horvath LB, Kuzevska I, Fairley CK. 2005. Evaluation of a point-of-care test, BVBlue, and clinical and laboratory criteria for diagnosis of bacterial vaginosis. J Clin Microbiol 43:13041308[CrossRef].[PubMed]
56. Myziuk L, Romanowski B, Johnson SC. 2003. BVBlue test for diagnosis of bacterial vaginosis. J Clin Microbiol 41:19251928[CrossRef].[PubMed]
57. Madhivanan P, Krupp K, Li T, Ravi K, Selezneva J, Srinivas V, Arun A, Klausner JD. 2014. Performance of BVBlue rapid test in detecting bacterial vaginosis among women in Mysore, India. Infect Dis Obstet Gynecol 2014:908313[CrossRef].[PubMed]
58. van Belkum A, Koeken A, Vandamme P, van Esbroeck M, Goossens H, Koopmans J, Kuijpers J, Falsen E, Quint W. 1995. Development of a species-specific polymerase chain reaction assay for Gardnerella vaginalis. Mol Cell Probes 9:167174[CrossRef].[PubMed]
59. Schwebke JR, Lawing LF. 2001. Prevalence of Mobiluncus spp among women with and without bacterial vaginosis as detected by polymerase chain reaction. Sex Transm Dis 28:195199[CrossRef].[PubMed]
60. Obata-Yasuoka M, Ba-Thein W, Hamada H, Hayashi H. 2002. A multiplex polymerase chain reaction-based diagnostic method for bacterial vaginosis. Obstet Gynecol 100:759764[CrossRef].[PubMed]
61. Burton JP, Reid G. 2002. Evaluation of the bacterial vaginal flora of 20 postmenopausal women by direct (Nugent score) and molecular (polymerase chain reaction and denaturing gradient gel electrophoresis) techniques. J Infect Dis 186:17701780[CrossRef].[PubMed]
62. Ferris MJ, Masztal A, Aldridge KE, Fortenberry JD, Fidel PL Jr, Martin DH. 2004. Association of Atopobium vaginae, a recently described metronidazole resistant anaerobe, with bacterial vaginosis. BMC Infect Dis 4:5[CrossRef].[PubMed]
63. Sha BE, Chen HY, Wang QJ, Zariffard MR, Cohen MH, Spear GT. 2005. Utility of Amsel criteria, Nugent score, and quantitative PCR for Gardnerella vaginalis, Mycoplasma hominis, and Lactobacillus spp. for diagnosis of bacterial vaginosis in human immunodeficiency virus-infected women. J Clin Microbiol 43:46074612[CrossRef].[PubMed]
64. Menard JP, Fenollar F, Raoult D, Boubli L, Bretelle F. 2012. Self-collected vaginal swabs for the quantitative real-time polymerase chain reaction assay of Atopobium vaginae and Gardnerella vaginalis and the diagnosis of bacterial vaginosis. Eur J Clin Microbiol Infect Dis 31:513518[CrossRef].[PubMed]
65. Zozaya-Hinchliffe M, Martin DH, Ferris MJ. 2008. Prevalence and abundance of uncultivated Megasphaera-like bacteria in the human vaginal environment. Appl Environ Microbiol 74:16561659[CrossRef].[PubMed]
66. Zozaya-Hinchliffe M, Lillis R, Martin DH, Ferris MJ. 2010. Quantitative PCR assessments of bacterial species in women with and without bacterial vaginosis. J Clin Microbiol 48:18121819[CrossRef].[PubMed]
67. Ling Z, Liu X, Luo Y, Wu X, Yuan L, Tong X, Li L, Xiang C. 2013. Associations between vaginal pathogenic community and bacterial vaginosis in Chinese reproductive-age women. PLoS One 8:e76589[CrossRef].[PubMed]
68. Dumonceaux TJ, Schellenberg J, Goleski V, Hill JE, Jaoko W, Kimani J, Money D, Ball TB, Plummer FA, Severini A. 2009. Multiplex detection of bacteria associated with normal microbiota and with bacterial vaginosis in vaginal swabs by use of oligonucleotide-coupled fluorescent microspheres. J Clin Microbiol 47:40674077[CrossRef].[PubMed]
69. Pépin J, Deslandes S, Giroux G, Sobéla F, Khonde N, Diakité S, Demeule S, Labbé AC, Carrier N, Frost E. 2011. The complex vaginal flora of West African women with bacterial vaginosis. PLoS One 6:e25082[CrossRef].[PubMed]
70. Datcu R, Gesink D, Mulvad G, Montgomery-Andersen R, Rink E, Koch A, Ahrens P, Jensen JS. 2013. Vaginal microbiome in women from Greenland assessed by microscopy and quantitative PCR. BMC Infect Dis 13:480[CrossRef].[PubMed]
71. Nelson DB, Komaroff E, Nachamkin I, Haggerty CL, Dibble L, Mastrogiannis D, Liu C, Fredricks DN. 2013. Relationship of selected bacterial vaginosis-associated bacteria to Nugent score bacterial vaginosis among urban women early in pregnancy. Sex Transm Dis 40:721723[CrossRef].[PubMed]
72. Fethers K, Twin J, Fairley CK, Fowkes FJ, Garland SM, Fehler G, Morton AM, Hocking JS, Tabrizi SN, Bradshaw CS. 2012. Bacterial vaginosis (BV) candidate bacteria: associations with BV and behavioural practices in sexually-experienced and inexperienced women. PLoS One 7:e30633[CrossRef].[PubMed]
73. Cartwright CP, Lembke BD, Ramachandran K, Body BA, Nye MB, Rivers CA, Schwebke JR. 2012. Development and validation of a semiquantitative, multitarget PCR assay for diagnosis of bacterial vaginosis. J Clin Microbiol 50:23212329[CrossRef].[PubMed]
74. Datcu R, Gesink D, Mulvad G, Montgomery-Andersen R, Rink E, Koch A, Ahrens P, Jensen JS. 2014. Bacterial vaginosis diagnosed by analysis of first-void-urine specimens. J Clin Microbiol 52:218225[CrossRef].[PubMed]

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