Chapter 38 : Molecular Detection of Gastrointestinal Parasites

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In parasitology, the importance of DNA-based testing methods became apparent in the 1990s following the redescription of the potentially invasive and the nonpathogenic (1). Since these two species are morphologically indistinguishable, nucleic acid-based detection methods were introduced in the clinical laboratory for their differentiation in cases where microscopy lacked specificity. In a similar way, molecular applications gradually replaced tests in which microscopy was time-consuming or inconclusive, for example, in the detection of microsporidia (2). When real-time PCR was introduced, the idea of using multiplex PCR assays for the simultaneous detection of various pathogens as first-line diagnosis gradually developed (3).

Citation: Verweij J, Van Belkum A, Stensvold C. 2016. Molecular Detection of Gastrointestinal Parasites, p 530-541. In Persing D, Tenover F, Hayden R, Ieven M, Miller M, Nolte F, Tang Y, van Belkum A (ed), Molecular Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555819071.ch38
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