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Chapter 12 : Isothermal Nucleic Acid Amplification Methods

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Isothermal Nucleic Acid Amplification Methods, Page 1 of 2

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Abstract:

In clinical virology, molecular diagnostics based on the direct detection of specific genetic material in a specimen through nucleic acid testing (NAT) has largely replaced antigen testing by immunoassays and has become the leading technology. Molecular test systems are more specific and sensitive. They are able to detect the presence of a pathogen earlier than an antigen immunoassay and are thus mainly used for diagnosing and screening patients for numerous viral diseases today.

Citation: Kessler H, Stelzl E. 2016. Isothermal Nucleic Acid Amplification Methods, p 137-144. In Loeffelholz M, Hodinka R, Young S, Pinsky B (ed), Clinical Virology Manual, Fifth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555819156.ch12
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References

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Tables

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TABLE 1

Isothermal nucleic acid amplification methods: Manufacturers and commercially available kits for detection and/or quantitation of viral DNA or RNA (updated August 2015)

Citation: Kessler H, Stelzl E. 2016. Isothermal Nucleic Acid Amplification Methods, p 137-144. In Loeffelholz M, Hodinka R, Young S, Pinsky B (ed), Clinical Virology Manual, Fifth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555819156.ch12
Generic image for table
TABLE 2

Strengths and weaknesses of isothermal nucleic acid amplification methods

Citation: Kessler H, Stelzl E. 2016. Isothermal Nucleic Acid Amplification Methods, p 137-144. In Loeffelholz M, Hodinka R, Young S, Pinsky B (ed), Clinical Virology Manual, Fifth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555819156.ch12

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