Chapter 4 : A Personal View of How Paleomicrobiology Aids Our Understanding of the Role of Lice in Plague Pandemics

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As early researchers in paleomicrobiology, we have used its techniques to study the plague, generating controversy after the publication of our initial results ( ). The controversy has allowed us to respond and propose new approaches to understanding plague pandemics. Our conclusion is that the plague was related to outbreaks of lice, not to rat fleas, because fleas cannot explain epidemics of this magnitude ( ).

Citation: Raoult D. 2016. A Personal View of How Paleomicrobiology Aids Our Understanding of the Role of Lice in Plague Pandemics, p 29-37. In Drancourt M, Raoult D (ed), Paleomicrobiology of Humans. ASM Press, Washington, DC. doi: 10.1128/microbiolspec.PoH-0001-2014
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Figure 1

Recovery of dental pulp from an ancient jaw. To view video go to http://www.asmscience.org/files/recovery_of_human_dental_pulp.mpg.

Citation: Raoult D. 2016. A Personal View of How Paleomicrobiology Aids Our Understanding of the Role of Lice in Plague Pandemics, p 29-37. In Drancourt M, Raoult D (ed), Paleomicrobiology of Humans. ASM Press, Washington, DC. doi: 10.1128/microbiolspec.PoH-0001-2014
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1. Houhamdi L,, Lepidi H,, Drancourt M,, Raoult D . 2006. Experimental model to evaluate the human body louse as a vector of plague. J Infect Dis 194 : 15891596. http://dx.doi.org/10.1086/508995. [PubMed] [CrossRef]
2. Bercovier M,, Mollaret HH, . 1984. Yersinia, p 498506. In Krieg NR,, Holt JG (ed), Bergey’s Manual of Systematic Bacteriology, vol 1. Williams & Wilkins, Baltimore, MD.
3. Drancourt M,, Houhamdi L,, Raoult D . 2006. Yersinia pestis as a telluric, human ectoparasite-borne organism. Lancet Infect Dis 6 : 234241. http://dx.doi.org/10.1016/S1473-3099(06)70438-8. [PubMed] [CrossRef]
4. Simond PL . 1898. La propagation de la peste. Ann Inst Pasteur (Paris) 10 : 625687.
5. Flatau G,, Lemichez E,, Gauthier M,, Chardin P,, Paris S,, Fiorentini C,, Boquet P . 1997. Toxin-induced activation of the G protein p21 Rho by deamidation of glutamine. Nature 387 : 729733. http://dx.doi.org/10.1038/42743. [CrossRef]
6. Baltazard M,, Aslani P . 1952. [Biochemical characteristics of the strains of wild plague in Kurdistan]. Ann Inst Pasteur (Paris) 83 : 241247.
7. Bitam I,, Ayyadurai S,, Kernif T,, Chetta M,, Boulaghman N,, Raoult D,, Drancourt M . 2010. New rural focus of plague, Algeria. Emerg Infect Dis 16 : 16391640. http://dx.doi.org/10.3201/eid1610.091854. [CrossRef]
8. Baltazard M . 1964. The conservation of plague in inveterate foci. J Hyg Epidemiol Microbiol Immunol 8 : 409421.[PubMed]
9. Baltazard M,, Mofidi C . 1950. Sur la peste inapparente des rongeurs sauvages. C R Acad Sci 231 : 731733.
10. Baltazard M,, Karimi Y,, Eftekhari M,, Chamsa M,, Mollaret HH . 1963. The interepizootic preservation of plague in an inveterate focus. Working hypotheses. Bull Soc Pathol Exot Filiales 56 : 12301245.[PubMed]
11. Blanc G,, Baltazard M . 1941. Recherches expérimentales sur la peste. L’infection du pou de l’homme Pediculus corporis de Geer. C R Acad Sci 213 : 849851.
12. Mollaret HH . 1963. Experimental preservation of plague in soil. Bull Soc Pathol Exot Filiales 56 : 11681182.[PubMed]
13. Begier EM,, Asiki G,, Anywaine Z,, Yockey B,, Schriefer ME,, Aleti P,, Ogden-Odoi A,, Staples JE,, Sexton C,, Bearden SW,, Kool JL . 2006. Pneumonic plague cluster, Uganda, 2004. Emerg Infect Dis 12 : 460467. http://dx.doi.org/10.3201/eid1203.051051. [PubMed] [CrossRef]
14. Drancourt M,, Aboudharam G,, Signoli M,, Dutour O,, Raoult D . 1998. Detection of 400-year-old Yersinia pestis DNA in human dental pulp: an approach to the diagnosis of ancient septicemia. Proc Natl Acad Sci U S A 95 : 1263712640. http://dx.doi.org/10.1073/pnas.95.21.12637. [PubMed] [CrossRef]
15. Aboudharam G,, Drancourt M,, Raoult D . 2004. Culture of C. burnetii from the dental pulp of experimentally infected guinea pigs. Microb Pathog 36 : 349350. http://dx.doi.org/10.1016/j.micpath.2004.02.002. [PubMed] [CrossRef]
16. Aboudharam G,, Lascola B,, Raoult D,, Drancourt M . 2000. Detection of Coxiella burnetii DNA in dental pulp during experimental bacteremia. Microb Pathog 28 : 249254. http://dx.doi.org/10.1006/mpat.1999.0343. [PubMed] [CrossRef]
17. Raoult D,, Aboudharam G,, Crubézy E,, Larrouy G,, Ludes B,, Drancourt M . 2000. Molecular identification by “suicide PCR” of Yersinia pestis as the agent of medieval black death. Proc Natl Acad Sci U S A 97 : 1280012803. http://dx.doi.org/10.1073/pnas.220225197. [PubMed] [CrossRef]
18. Duncan CJ,, Scott S . 2005. What caused the Black Death? Postgrad Med J 81 : 315320. http://dx.doi.org/10.1136/pgmj.2004.024075. [CrossRef]
19. Scott S,, Duncan CJ,, Duncan SR . 1996. The plague in Penrith, Cumbria, 1597/8: its causes, biology and consequences. Ann Hum Biol 23 : 121. http://dx.doi.org/10.1080/03014469600004232. [PubMed] [CrossRef]
20. Gilbert MT,, Cuccui J,, White W,, Lynnerup N,, Titball RW,, Cooper A,, Prentice MB . 2004. Absence of Yersinia pestis-specific DNA in human teeth from five European excavations of putative plague victims. Microbiology 150 : 341354. http://dx.doi.org/10.1099/mic.0.26594-0. [CrossRef]
21. Paterson R . 2002. Yersinia seeks pardon for Black Death. Lancet Infect Dis 2 : 323. http://dx.doi.org/10.1016/S1473-3099(02)00309-2. [CrossRef]
22. Raoult D,, Drancourt M . 2002. Cause of black death. Lancet Infect Dis 2 : 459. http://dx.doi.org/10.1016/S1473-3099(02)00341-9. [CrossRef]
23. Drancourt M,, Signoli M,, Dang LV,, Bizot B,, Roux V,, Tzortzis S,, Raoult D . 2007. Yersinia pestis Orientalis in remains of ancient plague patients. Emerg Infect Dis 13 : 332333. http://dx.doi.org/10.3201/eid1302.060197. [PubMed] [CrossRef]
24. Vergnaud G . 2005. Yersinia pestis genotyping. Emerg Infect Dis 11 : 13171318; author reply 1318–1319.[PubMed]
25. Schuenemann VJ,, Bos K,, DeWitte S,, Schmedes S,, Jamieson J,, Mittnik A,, Forrest S,, Coombes BK,, Wood JW,, Earn DJ,, White W,, Krause J,, Poinar HN . 2011. Targeted enrichment of ancient pathogens yielding the pPCP1 plasmid of Yersinia pestis from victims of the Black Death. Proc Natl Acad Sci U S A 108 : E746E752. http://dx.doi.org/10.1073/pnas.1105107108. [CrossRef]
26. Tran TN,, Signoli M,, Fozzati L,, Aboudharam G,, Raoult D,, Drancourt M . 2011. High throughput, multiplexed pathogen detection authenticates plague waves in medieval Venice, Italy. PLoS One 6 : e16735. http://dx.doi.org/10.1371/journal.pone.0016735. [CrossRef]
27. Tran TN,, Forestier CL,, Drancourt M,, Raoult D,, Aboudharam G . 2011. Brief communication: co-detection of Bartonella quintana and Yersinia pestis in an 11th-15th burial site in Bondy, France. Am J Phys Anthropol 145 : 489494. http://dx.doi.org/10.1002/ajpa.21510. [PubMed] [CrossRef]
28. Haensch S,, Bianucci R,, Signoli M,, Rajerison M,, Schultz M,, Kacki S,, Vermunt M,, Weston DA,, Hurst D,, Achtman M,, Carniel E,, Bramanti B . 2010. Distinct clones of Yersinia pestis caused the Black Death. PLoS Pathog 6 : e1001134. http://dx.doi.org/10.1371/journal.ppat.1001134. [PubMed] [CrossRef]
29. Wiechmann I,, Harbeck M,, Grupe G . 2010. Yersinia pestis DNA sequences in late medieval skeletal finds, Bavaria. Emerg Infect Dis 16 : 18061807. http://dx.doi.org/10.3201/eid1611.100598. [PubMed] [CrossRef]
30. Tran TN,, Raoult D,, Drancourt M . 2011. Yersinia pestis DNA sequences in late medieval skeletal finds, Bavaria. Emerg Infect Dis 17 : 955957; author reply 957. http://dx.doi.org/10.3201/eid1705.101777. [CrossRef]
31. Bos KI,, Schuenemann VJ,, Golding GB,, Burbano HA,, Waglechner N,, Coombes BK,, McPhee JB,, DeWitte SN,, Meyer M,, Schmedes S,, Wood J,, Earn DJ,, Herring DA,, Bauer P,, Poinar HN,, Krause J . 2011. A draft genome of Yersinia pestis from victims of the Black Death. Nature 478 : 506510. http://dx.doi.org/10.1038/nature10549. [PubMed] [CrossRef]
32. Gilbert MT . 2014. Yersinia pestis: one pandemic, two pandemics, three pandemics, more? Lancet Infect Dis 14 : 264265. http://dx.doi.org/10.1016/S1473-3099(14)70002-7. [PubMed] [CrossRef]
33. Wagner DM,, Klunk J,, Harbeck M,, Devault A,, Waglechner N,, Sahl JW,, Enk J,, Birdsell DN,, Kuch M,, Lumibao C,, Poinar D,, Pearson T,, Fourment M,, Golding B,, Riehm JM,, Earn DJ,, Dewitte S,, Rouillard JM,, Grupe G,, Wiechmann I,, Bliska JB,, Keim PS,, Scholz HC,, Holmes EC,, Poinar H . 2014. Yersinia pestis and the plague of Justinian 541-543 AD: a genomic analysis. Lancet Infect Dis 14 : 319326. http://dx.doi.org/10.1016/S1473-3099(13)70323-2. [CrossRef]
34. Molak M,, Ho SY . 2011. Evaluating the impact of post-mortem damage in ancient DNA: a theoretical approach. J Mol Evol 73 : 244255. http://dx.doi.org/10.1007/s00239-011-9474-z. [PubMed] [CrossRef]
35. Nguyen-Hieu T,, Aboudharam G,, Drancourt M . 2012. Heat degradation of eukaryotic and bacterial DNA: an experimental model for paleomicrobiology. BMC Res Notes 5 : 528. http://dx.doi.org/10.1186/1756-0500-5-528. [PubMed] [CrossRef]
36. Ayyadurai S,, Houhamdi L,, Lepidi H,, Nappez C,, Raoult D,, Drancourt M . 2008. Long-term persistence of virulent Yersinia pestis in soil. Microbiology 154 : 28652871. http://dx.doi.org/10.1099/mic.0.2007/016154-0. [PubMed] [CrossRef]
37. Adler CJ,, Dobney K,, Weyrich LS,, Kaidonis J,, Walker AW,, Haak W,, Bradshaw CJ,, Townsend G,, Soltysiak A,, Alt KW,, Parkhill J,, Cooper A . 2013. Sequencing ancient calcified dental plaque shows changes in oral microbiota with dietary shifts of the Neolithic and Industrial revolutions. Nat Genet 45 : 450455, 455e1.
38. Butler T . 2009. Plague into the 21st century. Clin Infect Dis 49 : 736742. http://dx.doi.org/10.1086/604718. [CrossRef]
39. Raoult D,, Roux V . 1999. The body louse as a vector of reemerging human diseases. Clin Infect Dis 29 : 888911. http://dx.doi.org/10.1086/520454. [CrossRef]
40. Fournier PE,, Minnick MF,, Lepidi H,, Salvo E,, Raoult D . 2001. Experimental model of human body louse infection using green fluorescent protein-expressing Bartonella quintana . Infect Immun 69 : 18761879. http://dx.doi.org/10.1128/IAI.69.3.1876-1879.2001. [CrossRef]
41. Houhamdi L,, Raoult D . 2005. Excretion of living Borrelia recurrentis in feces of infected human body lice. J Infect Dis 191 : 18981906. http://dx.doi.org/10.1086/429920. [PubMed] [CrossRef]
42. Houhamdi L,, Fournier PE,, Fang R,, Raoult D . 2003. An experimental model of human body louse infection with Rickettsia typhi . Ann N Y Acad Sci 990 : 617627. http://dx.doi.org/10.1111/j.1749-6632.2003.tb07436.x. [CrossRef]
43. Houhamdi L,, Fournier PE,, Fang R,, Lepidi H,, Raoult D . 2002. An experimental model of human body louse infection with Rickettsia prowazekii . J Infect Dis 186 : 16391646. http://dx.doi.org/10.1086/345373. [PubMed] [CrossRef]
44. Fang R,, Houhamdi L,, Raoult D . 2002. Detection of Rickettsia prowazekii in body lice and their feces by using monoclonal antibodies. J Clin Microbiol 40 : 33583363. http://dx.doi.org/10.1128/JCM.40.9.3358-3363.2002. [PubMed] [CrossRef]
45. Houhamdi L,, Raoult D . 2008. Different genes govern Yersinia pestis pathogenicity in Caenorhabditis elegans and human lice. Microb Pathog 44 : 435437. http://dx.doi.org/10.1016/j.micpath.2007.11.007. [CrossRef]
46. Raoult D,, Ndihokubwayo JB,, Tissot-Dupont H,, Roux V,, Faugere B,, Abegbinni R,, Birtles RJ . 1998. Outbreak of epidemic typhus associated with trench fever in Burundi. Lancet 352 : 353358. http://dx.doi.org/10.1016/S0140-6736(97)12433-3. [CrossRef]
47. Raoult D,, Dutour O,, Houhamdi L,, Jankauskas R,, Fournier PE,, Ardagna Y,, Drancourt M,, Signoli M,, La VD,, Macia Y,, Aboudharam G . 2006. Evidence for louse-transmitted diseases in soldiers of Napoleon’s Grand Army in Vilnius. J Infect Dis 193 : 112120. http://dx.doi.org/10.1086/498534. [CrossRef]
48. Piarroux R,, Abedi AA,, Shako JC,, Kebela B,, Karhemere S,, Diatta G,, Davoust B,, Raoult D,, Drancourt M . 2013. Plague epidemics and lice, Democratic Republic of the Congo. Emerg Infect Dis 19 : 505506. http://dx.doi.org/10.3201/eid1903.121542. [CrossRef]
49. Anonymous . 1997. A large outbreak of epidemic louse-borne typhus in Burundi. Wkly Epidemiol Rec 72 : 152153.[PubMed]
50. Raoult D,, Roux V,, Ndihokubwayo JB,, Bise G,, Baudon D,, Marte G,, Birtles R . 1997. Jail fever (epidemic typhus) outbreak in Burundi. Emerg Infect Dis 3 : 357360. http://dx.doi.org/10.3201/eid0303.970313. [CrossRef]
51. Anonymous . 1994. Epidemic typhus risk in Rwandan refugee camps. Wkly Epidemiol Rec 69 : 259. [PubMed]

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