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Chapter 12 : Fungal and Mycobacterial Blood Cultures

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Abstract:

Early and appropriate antimicrobial therapy of sepsis is associated with improved clinical outcomes, and the laboratory identification of the etiological agent of fungemia and/or mycobacteremia is very important for successful outcome. Fungi and some mycobacteria grow more slowly than many common pathogenic bacteria, and specialized broth culture media and methodologies are available for their isolation. Not surprisingly, there are reports of fungi, primarily yeast, being recovered more effectively using mycobacterial blood culture media compared with routine blood culture bottles intended for the detection of aerobic and anaerobic bacteria ( ). The laboratory diagnosis of mycobacteremia and fungemia often requires special consideration with the selection of blood culture testing and interpretation of results.

Citation: Liao R, Lainhart W. 2017. Fungal and Mycobacterial Blood Cultures, p 245-276. In Dunne, Jr. W, Burnham C (ed), The Dark Art of Blood Cultures. ASM Press, Washington, DC. doi: 10.1128/9781555819811.ch12
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Figure 1

Gram stains of positive fungal blood cultures. (1000× magnification). (Middle) (1000× magnification) sp. (1000× magnification).

Citation: Liao R, Lainhart W. 2017. Fungal and Mycobacterial Blood Cultures, p 245-276. In Dunne, Jr. W, Burnham C (ed), The Dark Art of Blood Cultures. ASM Press, Washington, DC. doi: 10.1128/9781555819811.ch12
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Image of Figure 2
Figure 2

Exemplar images of mycobacterial blood culture media. (A) Thermo Scientific VersaTREK Myco blood culture bottle (front, center) and the Thermo Scientific VersaTREK Myco susceptibility kit ( ). Photos courtesy of Thermo Fisher Scientific. Copying is prohibited. (B) BBL Septi-Chek AFB biphasic mycobacterial culture system ( ).

Citation: Liao R, Lainhart W. 2017. Fungal and Mycobacterial Blood Cultures, p 245-276. In Dunne, Jr. W, Burnham C (ed), The Dark Art of Blood Cultures. ASM Press, Washington, DC. doi: 10.1128/9781555819811.ch12
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Table 1

Summary of studies comparing various media types and automated systems for the recovery and average time to positivity of mycobacterial blood cultures

Citation: Liao R, Lainhart W. 2017. Fungal and Mycobacterial Blood Cultures, p 245-276. In Dunne, Jr. W, Burnham C (ed), The Dark Art of Blood Cultures. ASM Press, Washington, DC. doi: 10.1128/9781555819811.ch12

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