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Chapter 3 : Lysis-Centrifugation Methods of Blood Culture

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Abstract:

In 1917, Mildred Clough first demonstrated the usefulness of lysis-centrifugation blood culture when she used it to isolate from blood ( ). Using this processing method, she was able to recover colonies of on solid agar media in just two weeks. The process was complex and time-consuming, and, despite the desirable results, lysis-centrifugation blood culture was largely abandoned until the mid-1970s.

Citation: TeKippe E, Pence M. 2017. Lysis-Centrifugation Methods of Blood Culture, p 39-57. In Dunne, Jr. W, Burnham C (ed), The Dark Art of Blood Cultures. ASM Press, Washington, DC. doi: 10.1128/9781555819811.ch3
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Figure 1

Wampole Isolator 1.5-ml microbial tube and Isolator 10 tube. Image courtesy of Dr. Dominick Cavuoti, used with permission from Alere.

Citation: TeKippe E, Pence M. 2017. Lysis-Centrifugation Methods of Blood Culture, p 39-57. In Dunne, Jr. W, Burnham C (ed), The Dark Art of Blood Cultures. ASM Press, Washington, DC. doi: 10.1128/9781555819811.ch3
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Image of Figure 2
Figure 2

Isostat Press and Rack for processing of Isolator 10 tubes. Image used with permission from Alere.

Citation: TeKippe E, Pence M. 2017. Lysis-Centrifugation Methods of Blood Culture, p 39-57. In Dunne, Jr. W, Burnham C (ed), The Dark Art of Blood Cultures. ASM Press, Washington, DC. doi: 10.1128/9781555819811.ch3
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References

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