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Chapter 6 : TREK Blood Culture Systems

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TREK Blood Culture Systems, Page 1 of 2

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Abstract:

Over the past three-plus decades, Difco Laboratories (Detroit, MI) and then TREK Diagnostic Systems (Thermo Scientific Microbiology, Waltham, MA) have developed several iterations of continuous-monitoring blood culture systems (CMBCSs). Similar to other CMBCSs, these systems allow for incubation of inoculated blood culture bottles with simultaneous monitoring of positivity by indirect measurement of bacterial growth. However, the unique approach toward monitoring growth distinguishes the TREK systems from other CMBCSs.

Citation: Anderson N, Yarbrough M. 2017. TREK Blood Culture Systems, p 113-136. In Dunne, Jr. W, Burnham C (ed), The Dark Art of Blood Cultures. ASM Press, Washington, DC. doi: 10.1128/9781555819811.ch6
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Figures

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Figure 1

Developmental history of manometric continuous-monitoring blood culture systems. This timeline highlights the major historical developments leading to the widespread utilization of manometric continuous-monitoring systems for blood culture monitoring, spp. detection and spp. resistance testing.

Citation: Anderson N, Yarbrough M. 2017. TREK Blood Culture Systems, p 113-136. In Dunne, Jr. W, Burnham C (ed), The Dark Art of Blood Cultures. ASM Press, Washington, DC. doi: 10.1128/9781555819811.ch6
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Image of Figure 2
Figure 2

ESP continuous-monitoring blood culture system. This figure depicts the Difco ESP continuous-monitoring blood culture system. Incubator sizes ranged from 128 to 384 bottle capacities and were arranged as drawers that could be pulled open from the front to reveal bottle stations. Lower drawers would continuously agitate the bottles and were reserved for aerobic bottles. Upper drawers did not agitate the bottles and were used for anaerobic bottles.

Citation: Anderson N, Yarbrough M. 2017. TREK Blood Culture Systems, p 113-136. In Dunne, Jr. W, Burnham C (ed), The Dark Art of Blood Cultures. ASM Press, Washington, DC. doi: 10.1128/9781555819811.ch6
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Image of Figure 3
Figure 3

VersaTREK continuous-monitoring blood culture system. This figure depicts the VersaTREK continuous-monitoring blood culture system. Individual drawers are opened by pulling forward on the handles present on the front of the instrument, revealing a series of bottle-loading stations. Each bottle-loading station is capable of accommodating aerobic, anaerobic, spp. isolation, and susceptibility testing media. Thermo Scientific VersaTREK Automated Microbial Detection System is owned by Thermo Fisher Scientific. Copying is prohibited.

Citation: Anderson N, Yarbrough M. 2017. TREK Blood Culture Systems, p 113-136. In Dunne, Jr. W, Burnham C (ed), The Dark Art of Blood Cultures. ASM Press, Washington, DC. doi: 10.1128/9781555819811.ch6
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Image of Figure 4
Figure 4

VersaTREK blood culture media. This figure depicts uninoculated blood culture media available for use on the VersaTREK instrument: Aerobic REDOX 1 bottle, Anaerobic REDOX 2 bottle, Aerobic REDOX 1 EZ Draw bottle, and Anaerobic REDOX 2 bottle. Thermo Scientific VersaTREK REDOX Media is owned by Thermo Fisher Scientific. Copying is prohibited.

Citation: Anderson N, Yarbrough M. 2017. TREK Blood Culture Systems, p 113-136. In Dunne, Jr. W, Burnham C (ed), The Dark Art of Blood Cultures. ASM Press, Washington, DC. doi: 10.1128/9781555819811.ch6
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Figure 5

Summary of VersaTREK workflow. This figure illustrates the typical workflow for the preparation and loading of inoculated blood cultures onto the VersaTREK instrument. The described steps are the same for all available blood culture media, regardless of formulation. All bottle-loading stations are capable of incubating both aerobic and anaerobic media.

Citation: Anderson N, Yarbrough M. 2017. TREK Blood Culture Systems, p 113-136. In Dunne, Jr. W, Burnham C (ed), The Dark Art of Blood Cultures. ASM Press, Washington, DC. doi: 10.1128/9781555819811.ch6
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Figure 6

Example of pressure readings obtained over time for a positive blood culture by the VersaTREK system. Growth of microorganisms results in changes in headspace pressure that is detected by the instrument. The user can graphically view changes in pressure using the VersaTREK software (solid line on graph). Bottle events, such as a positive signal, are indicated in red.

Citation: Anderson N, Yarbrough M. 2017. TREK Blood Culture Systems, p 113-136. In Dunne, Jr. W, Burnham C (ed), The Dark Art of Blood Cultures. ASM Press, Washington, DC. doi: 10.1128/9781555819811.ch6
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Tables

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Table 1

VersaTREK media components ( )

Citation: Anderson N, Yarbrough M. 2017. TREK Blood Culture Systems, p 113-136. In Dunne, Jr. W, Burnham C (ed), The Dark Art of Blood Cultures. ASM Press, Washington, DC. doi: 10.1128/9781555819811.ch6

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