Electrophoresis
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6 results
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Improvement in Student Data Analysis Skills after Out-of-Class Assignments †
- Author: Kristen L.W. Walton
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Citation: Walton K. 2016. Improvement in student data analysis skills after out-of-class assignments † . 17(3):466-468 doi:10.1128/jmbe.v17i3.1107
- DOI 10.1128/jmbe.v17i3.1107
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Abstract:
The ability to understand and interpret data is a critical aspect of scientific thinking. However, although data analysis is often a focus in biology majors classes, many textbooks for allied health majors classes are primarily content-driven and do not include substantial amounts of experimental data in the form of graphs and figures. In a lower-division allied health majors microbiology class, students were exposed to data from primary journal articles as take-home assignments and their data analysis skills were assessed in a pre-/posttest format. Students were given 3 assignments that included data analysis questions. Assignments ranged from case studies that included a figure from a journal article to reading a short journal article and answering questions about multiple figures or tables. Data were represented as line or bar graphs, gel photographs, and flow charts. The pre- and posttest was designed incorporating the same types of figures to assess whether the assignments resulted in any improvement in data analysis skills. The mean class score showed a small but significant improvement from the pretest to the posttest across three semesters of testing. Scores on individual questions testing accurate conclusions and predictions improved the most. This supports the conclusion that a relatively small number of out-of-class assignments through the semester resulted in a significant improvement in data analysis abilities in this population of students.
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Two Laboratory Activities Using Conventional or Real-Time PCR to Simulate Pathogenic E. coli Detection †
- Author: Joanna R. Klein
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Citation: Klein J. 2014. Two laboratory activities using conventional or real-time pcr to simulate pathogenic e. coli detection † . 15(1):51-52 doi:10.1128/jmbe.v15i1.665
- DOI 10.1128/jmbe.v15i1.665
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In these lab activities, students perform conventional PCR and real-time PCR to simulate pathogenic E. coli detection. The labs were designed to complement a previously published virtual PCR classroom activity in which students are asked to design a PCR-based diagnostic test for a pathogenic strain of E. coli. In the virtual PCR activity, students use bioinformatics to discover that the Shiga toxin genes (stx1 and stx2) are unique to the Enterohemorrhagic E. coli (EHEC) strain O157:H7. Thus they come to the conclusion that doing PCR with primers designed for shiga toxin should be able to differentiate O157:H7 from other strains of E. coli. In the lab activity described here, students actually perform the PCR assay. Performing PCR enhanced student understanding of the technique beyond what was accomplished through the virtual PCR classroom activity and is recommended as an addition to the case study.
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Engaging Students in Authentic Microbiology Research in an Introductory Biology Laboratory Course is Correlated with Gains in Student Understanding of the Nature of Authentic Research and Critical Thinking †
- Authors: Brittany J. Gasper, Stephanie M. Gardner*
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Citation: Gasper B, Gardner S. 2013. Engaging students in authentic microbiology research in an introductory biology laboratory course is correlated with gains in student understanding of the nature of authentic research and critical thinking † . 14(1):25-34 doi:10.1128/jmbe.v14i1.460
- DOI 10.1128/jmbe.v14i1.460
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Abstract:
Recent recommendations for biology education highlight the role of authentic research experiences early in undergraduate education as a means of increasing the number and quality of biology majors. These experiences will inform students on the nature of science, increase their confidence in doing science, as well as foster critical thinking skills, an area that has been lacking despite it being one of the desired outcomes at undergraduate institutions and with future employers. With these things in mind, we have developed an introductory biology laboratory course where students design and execute an authentic microbiology research project. Students in this course are assimilated into the community of researchers by engaging in scholarly activities such as participating in inquiry, reading scientific literature, and communicating findings in written and oral formats. After three iterations of a semester-long laboratory course, we found that students who took the course showed a significant increase in their understanding of the nature of authentic research and their level of critical thinking skills.
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Research, Collaboration, and Open Science Using Web 2.0
- Authors: Kevin Shee*, Michael Strong*, Nicholas J. Guido, Robert A. Lue, George M. Church, Alain Viel
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Citation: Shee K, Strong M, Guido N, Lue R, Church G, Viel A. 2010. Research, collaboration, and open science using web 2.0. 11(2):130-134 doi:10.1128/jmbe.v11i2.219
- DOI 10.1128/jmbe.v11i2.219
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Abstract:
There is little doubt that the Internet has transformed the world in which we live. Information that was once archived in bricks and mortar libraries is now only a click away, and people across the globe have become connected in a manner inconceivable only 20 years ago. Although many scientists and educators have embraced the Internet as an invaluable tool for research, education and data sharing, some have been somewhat slower to take full advantage of emerging Web 2.0 technologies. Here we discuss the benefits and challenges of integrating Web 2.0 applications into undergraduate research and education programs, based on our experience utilizing these technologies in a summer undergraduate research program in synthetic biology at Harvard University. We discuss the use of applications including wiki-based documentation, digital brainstorming, and open data sharing via the Web, to facilitate the educational aspects and collaborative progress of undergraduate research projects. We hope to inspire others to integrate these technologies into their own coursework or research projects.
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Restriction Analysis Challenge
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Source: Molecular Biology and Biotechnology: A Guide for Students, Third Edition , pp 202-204
Publication Date :
January 2008
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Restriction analysis is especially important in checking the structures of recombinant DNA molecules and in analyzing an unknown DNA. This chapter shows some examples with some typical restriction analysis problems encountered by scientists in the laboratory. Observing the sizes of fragments produced, scientists determine restriction site locations that would give them the patterns they see. A linear (not circular) piece of DNA is digested with the restriction endonuclease EcoRI and gives fragments of the following sizes: 3,000, 3,600, and 3,400 bp. When digested with BamHI, the DNA molecule gives fragments of the following sizes: 4,500, 3,000, and 2,500 bp. A double digest with EcoRI and BamHI gives fragments of the following sizes: 2,500, 500, 3,600, 3,000, and 400 bp. The distances between the restriction sites in base pairs are indicated, and the sites EcoRI or BamHI are appropriately labeled.
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Molecular Typing of Legionella pneumophila by Pulsed-Field Gel Electrophoresis and Amplified Fragment Length Polymorphism Analysis
- Authors: W. J. B. Wannet, W. K. van der Zwaluw, M. E. O. C. Heck, C. E. Elzenaar, H. Μ. E. Maas, H. Brunings, J. F. P. Schellekens, A. M. C. Bergmans, A. van der Zee, E. Thijssen, M. F. Peeters
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Source: Legionella , pp 260-262
Publication Date :
January 2002
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When detailed epidemiologic characterization is required, standardized molecular typing methods are indispensable for the subtyping of Legionella pneumophila serogroups. Therefore, a comparison was made between pulsed-field gel electrophoresis (PFGE) and amplified fragment length polymorphism (AFLP) analysis for genotyping of L. pneumophila, using standardized protocols from the European Working Group on Legionella Infections (EWGLI). DNA fingerprint patterns derived from AFLP seem to be superior to the patterns derived from PFGE, in both speed and interpretability. AFLP and PFGE have similar discriminatory power. AFLP and PFGE are powerful tools for the epidemiological subtyping of L. pneumophila. The authors emphasize the importance of the standardization of data analysis and data exchange using the latest software (BioNumerics). Studies are under way to determine both the intralaboratory and interlaboratory reproducibility more extensively for further standardization of these powerful subtyping methods used by an increasing number of European countries.