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A Laboratory Project Investigating Phenotypic Diversity Among Soilborne Streptomycetes

  • Authors: Kim Mogen1, Linda Kinkel2
  • VIEW AFFILIATIONS HIDE AFFILIATIONS
    Affiliations: 1: University of Wisconsin-River Falls; 2: University of Minnesota
  • Citation: Kim Mogen, Linda Kinkel. 2006. A laboratory project investigating phenotypic diversity among soilborne streptomycetes.
  • Publication Date : October 2006
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In this project the phenotypic diversity (antibiotic resistance, inhibition, pigmentation, and hyphal structure) of antibiotic-producing streptomycetes isolated from varying soils is examined. As a team, students (junior- and senior-level undergraduates) prepare a dilution series and perform a plate count on their soil. As individuals, students identify, purify, and make spore suspensions of the four best antibiotic-producing streptomycete isolates from their soil sample. They challenge their streptomycete isolates against antibiotic-containing discs, gram-positive and gram-negative bacteria, and other streptomycetes isolated from their soil. As a team, students prepare short oral reports, collect data, and present their experimental results.

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