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Genetic and Physical Mapping of a Bacterial Genome

  • Authors: Brad Goodner1, Cathy Wheeler2
  • VIEW AFFILIATIONS HIDE AFFILIATIONS
    Affiliations: 1: Department of Biology, Hiram College; 2: Department of Biology, Hiram College
  • Citation: Brad Goodner, Cathy Wheeler. 2005. Genetic and physical mapping of a bacterial genome.
  • Publication Date : January 2005
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In this three-component project, students (i) use a transposon to generate mutants that they screen for auxotrophs and characterize through biochemical complementation, (ii) use rare cutting restriction enzymes and pulsed-field gel electrophoresis to physically map the transposon insertions, and (iii) recover the transposon insertions for restriction mapping and subsequent sequencing in order to generate a genetic and physical map of a bacterial chromosome. The focus is on student involvement in original research.

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