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HeLa Cell Morphology

  • Authors: Patrick J. Cummings 1, Kristina M. Obom 2
  • VIEW AFFILIATIONS HIDE AFFILIATIONS
    Affiliations: 1: Johns Hopkins University, Baltimore and Rockville, MD, 21218; 2: Johns Hopkins University, Baltimore and Rockville, Maryland, 21218
  • Citation: Patrick J. Cummings, Kristina M. Obom. 2007. Hela cell morphology.
  • Publication Date : June 2007
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Introduction



Figure shows a monolayer (~50% of plastic surface covered with cells) of HeLa cells with typical morphology under low magnification using phase-contrast microscopy (40X objective). Note the subconfluent monolayer of cells with the unoccupied surface and cell boundary and the condensed nuclear chromatin.




Methods



The phase-contrast microscopic images show the typical cellular morphology of HeLa cells grown in vitro in cell culture medium. The HeLa cells were cultivated at 37oC under 5% CO2 in Dulbecco modified Eagle medium containing 5% fetal calf serum.



Discussion



HeLa cells (1) are a human cervical cancer cell line used in research and industrial settings to investigate cell biology, cancer, and virus propagation.

Reference



1. Gey, G. O., et al.
1952. Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium. Cancer Res. 12:264ֲ65.  

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