The bacteria were grown at room temperature on low-energy medium after a serial dilution of an Iowa soil. The Azotobacter plates were prepared by adding 1% mannitol to a moist soil and incubating at room temperature for 2 weeks. The video was captured using bright-field microscopy and captions were added using Adobe Premiere.
Coyne, M. S. 1999. Soil microbiology: an exploratory approach. Delmar Publishers, Albany, NY.
Sylvia, D. M., J. J. Fuhrmann, P. G. Hartel, and D. A. Zuberer. 2005. Principles and applications of soil microbiology. Pearson Prentice Hall, Upper Saddle River, NJ.