1887

Gram Stain of Urethral Discharge with Intracellular Neisseria gonorrhoeae

  • Authors: Javier Gutierrez Jimenez 1, Carlos Alberto Balcazar-Reyes 2, Juan de Dios Flores-Hernandez 3
  • VIEW AFFILIATIONS HIDE AFFILIATIONS
    Affiliations: 1: Lab. de Biologia Molecular y Genetica, Facultad de Ciencias Biologicas, Universidad de Ciencias y Artes de Chiapas, Tuxtla Gutierrez, Chiapas, 29000; 2: Lab. de Biologia Molecular y Genetica, Facultad de Ciencias Biologicas, Universidad de Ciencias y Artes de Chiapas, Tuxtla Gutierrez, Chiapas, 29000; 3: Lab. de Biologia Molecular y Genetica, Facultad de Ciencias Biologicas, Universidad de Ciencias y Artes de Chiapas, Tuxtla Gutierrez, Chiapas, 29000
  • Citation: Javier Gutierrez Jimenez, Carlos Alberto Balcazar-Reyes, Juan de Dios Flores-Hernandez. 2009. Gram stain of urethral discharge with intracellular neisseria gonorrhoeae.
  • Publication Date : August 2009
MyBook is a cheap paperback edition of the original book and will be sold at uniform, low price.
Add to My Favorites
You must be logged in to use this functionality
Introduction



The direct Gram stain of a urethral discharge from males with urethritis is a rapid and sensitive screening test for Neisseria gonorrhoeae
infection. This image shows gram-negative diplococci within polymorphonuclear leukocytes. Cocci are 0.6 to 1.0 mm in diameter and can occur singly, but are more often found in pairs with adjacent sides flattened (diplococci). Bacterial culture and carbohydrate fermentation studies provide definitive identification of the organism. N. gonorrhoeae is the etiological agent of the sexually transmitted disease gonorrhea. Infections caused by this organism are localized to the mucosal surfaces in the area of initial exposure to the organism (e.g., cervix, conjunctiva, pharyngeal surface, anorectal area, or urethra of males). The organism may also be spread from infected mother to newborn during birth.


Methods

 
This image is of a Gram stain of urethral discharge from a symptomatic male with urethritis. A urethral discharge was collected by inserting a flexible swab 2 to 4 cm into the urethra and rotating for 2 seconds.  Then the specimen collected was rolled directly onto the surface of a clean, dry glass slide, heat fixed, stained using Gram stain technique, and examined under an oil immersion objective. After examination, stained slides should be discarded in an appropriate container.

References

1.  Forbes, B. A., D. F. Sahm, and A. S. Weissfeld.  2002.  Bailey & Scott´s diagnostic microbiology, p. 502–509.  Mosby, St. Louis, MO.

2.  Whiley, D. M., J. W. Tapsall, and T. P. Sloots.  2006.  Nucleic acid amplification testing for Neisseria gonorrhoeae: an ongoing challenge.  J. Mol. Diagn. 8:3–15.

Related Resources

This is a required field
Please enter a valid email address
Please check the format of the address you have entered.
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error