1887

Eosin-Methylene Blue Agar Plates Protocol

  • Authors: Archana Lal 1, Naowarat Cheeptham 2
  • VIEW AFFILIATIONS HIDE AFFILIATIONS
    Affiliations: 1: Independence Community College, Independence, KS, 67301; 2: Thompson Rivers University, Kamloops, BC V2C 5N3
  • Citation: Archana Lal, Naowarat Cheeptham. 2007. Eosin-methylene blue agar plates protocol.
  • Publication Date : September 2007
  • Category: Protocol
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Figure 1

FIG. 1. Nutrient agar inoculated with Escherichia coli (a gram-negative bacterium) demonstrating growth with whitish colonies.

Source: September 2007
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Figure 2

FIG. 2. Eosin-methylene blue ( EMB) agar plate inoculated with Escherichia coli (a gram-negative coliform bacterium) showing good growth of dark blue-black colonies with metallic green sheen indicating vigorous fermentation of lactose and acid production which precipitates the green metallic pigment. (Naowarat Cheeptham, Thompson Rivers University, Kamloops, BC, Canada)

Source: September 2007
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Figure 3

FIG. 3. Nutrient agar inoculated with Pseudomonas aeruginosa (a gram-negative bacterium) demonstrating growth with whitish colonies.

Source: September 2007
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Figure 4

FIG. 4. EMB agar plate inoculated with Pseudomonas aeruginosa (a gram-negative noncoliform bacterium) showing good growth but no fermentation of sugars or acid production. (Naowarat Cheeptham and Carolynne Fardy, Thompson Rivers University, Kamloops, BC, Canada)

Source: September 2007
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Figure 5

FIG. 5. Nutrient agar inoculated with Staphylococcus aureus (a gram-positive bacterium) demonstrating growth with whitish colonies.

Source: September 2007
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Figure 6

Source: September 2007
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