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Mannitol Salt Agar Plates Protocols

  • Authors: Patricia Shields 1, Anne Y. Tsang 2
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    Affiliations: 1: Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD, 20742; 2: formerly of Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD, 20742
  • Citation: Patricia Shields, Anne Y. Tsang. 2006. Mannitol salt agar plates protocols.
  • Publication Date : October 2006
  • Category: Protocol
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Figure 1

FIG. 1. Tryptic soy agar (TSA) inoculated with (A) Staphylococcus aureus, (B) Staphylococcus epidermidis, and (C) Escherichia coli demonstrating growth of all three organisms. TSA is a general purpose medium that will allow for the growth of all three organisms.

Source: October 2006
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Figure 2

FIG. 2. (A) Staphylococcus aureus, (B) Staphylococcus epidermidis, and (C) Escherichia coli streaked on a mannitol salt agar plate. The mannitol fermenting colony (yellow) is S. aureus, while the mannitol nonfermenting colony (pink) is S. epidermidis. The growth of E. coli was inhibited by the high salt concentration.

Source: October 2006
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