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Oxidative-Fermentative Test Protocol

  • Author: Anne Hanson 1
  • VIEW AFFILIATIONS HIDE AFFILIATIONS
    Affiliations: 1: Department of Biochemistry, Microbiology and Molecular Biology, University of Maine, Orono, ME, 04469
  • Citation: Anne Hanson. 2008. Oxidative-fermentative test protocol.
  • Publication Date : September 2008
  • Category: Protocol
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Source: September 2008
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FIG. 9. Oxidative-fermentative test with glucose: oxidative result for Pseudomonas aeruginosa. Acid production in the open (aerobic) tube and not the oil-covered (anaerobic) tube indicates an oxidative result.



(a). P. aeruginosa incubated for 24 hours at 37°C. Note pH change in the top of the open (aerobic) tube only.



(b). P. aeruginosa incubated for 48 hours at 37°C. Note the diffusion of the acid further down the open (aerobic) tube.



(c). P. aeruginosa incubated for 5 days. Note the diffusion of the acid throughout the open (aerobic) tube.



The result is best if read within 24 to 48 hours. (Anne Hanson, University of Maine, Orono, ME)

Source: September 2008
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FIG. 6. Oxidative-fermentative test with glucose: Alcaligenes faecalis after a 24-hour incubation at 37°C. This result indicates that the bacteria are nonsaccharolytic and unable to use glucose either oxidatively or fermentatively. Note the absence of color change in the oil-covered (anaerobic) tube. The alkaline conditions and growth at the top of the open (aerobic) tube are a result of an increase in pH due to amine production as the bacteria is using the peptones in the media and not the glucose.(Anne Hanson, University of Maine, Orono, ME)

Source: September 2008
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