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Coagulase Test Protocol

  • Author: D. Sue Katz1
  • VIEW AFFILIATIONS HIDE AFFILIATIONS
    Affiliations: 1: Biology Department, Rogers State University, Claremore, OK, 74017
  • Citation: D. Sue Katz. 2010. Coagulase test protocol.
  • Publication Date : November 2010
  • Category: Protocol
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Figures

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Figure 1

Slide coagulase test results. (Right) A positive slide coagulase test result for Staphylococcus aureus when mixed with coagulase plasma. A positive reaction is indicated by visible clumping of Staphylococcus aureus. (Left) A negative slide coagulase test result for Staphylococcus epidermidis when mixed with coagulase plasma. A negative reaction is indicated by no visible clumping. (D. Sue Katz, Rogers State University, Claremore, OK)

Source: November 2010
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Figure 2

Coagulase-negative staphylococci. Note the individual cells and lack of large clumps of cells. Cells on the slide coagulase test slide were dried, heat fixed, and stained with methylene blue. Photograph is at 1,000X magnification. (D. Sue Katz, Rogers State University, Claremore, OK)

Source: November 2010
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Figure 3

Coagulase-positive staphylococci. While some individual cells are observed, the majority of cells are gathered into a large clump, large enough to be visible to the naked eye. Cells on the slide coagulase test slide were dried, heat fixed, and stained with methylene blue. Photograph is at 1,000X magnification. (D. Sue Katz, Rogers State University, Claremore, OK)

Source: November 2010
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Figure 4

A negative tube coagulase test result given by Staphylococcus epidermidis after a 24-hour incubation in coagulase plasma. A negative reaction is indicated by a lack of clotting in the tilted tube. (D. Sue Katz, Rogers State University, Claremore, OK)

Source: November 2010
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Figure 5

A positive tube coagulase test result for Staphylococus aureus after a 24-hour incubation in coagulase plasma. A positive result is indicated by clotting in the tilted tube. (D. Sue Katz, Rogers State University, Claremore, OK)



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Source: November 2010
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