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Hektoen Enteric Agar Protocol

  • Author: Jan Hudzicki1
  • VIEW AFFILIATIONS HIDE AFFILIATIONS
    Affiliations: 1: University of Kansas Medical Center, Kansas City, KS, 66160
  • Citation: Jan Hudzicki. 2010. Hektoen enteric agar protocol.
  • Publication Date : November 2010
  • Category: Protocol
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Figures

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Figure 1

Salmonella enterica on Hektoen enteric agar. Note the black center of the transparent colonies, indicating H2S production in the absence of carbohydrate fermentation. All serotypes of Salmonella have this appearance on Hektoen enteric agar except for serotype Typhi, which is a weak H2S producer. (Jan Hudzicki, University of Kansas Medical Center, Kansas City, KS)

Source: November 2010
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Figure 2

Shigella flexneri on Hektoen enteric agar. Note the transparent colonies, indicating the absence of carbohydrate fermentation. The lack of black colonies indicates no H2S production. All species of Shigella have this appearance on Hektoen enteric agar. (Jan Hudzicki, University of Kansas Medical Center, Kansas City, KS)

Source: November 2010
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Figure 3

Enterobacter aerogenes on Hektoen enteric agar. Note the yellow-orange colonies, indicating the fermentation of at least one of the carbohydrates present in the medium. The lack of black colonies indicates no H2S production. The orange haze around the colonies is due to the precipitation of the bile salts by the organism. The appearance of E. aerogenes on Hektoen enteric agar is typical of most nonpathogenic enteric gram-negative rods. (Jan Hudzicki, University of Kansas Medical Center, Kansas City, KS)

Source: November 2010
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Figure 4

Proteus vulgaris on Hektoen enteric agar. Note the yellow-orange colonies, indicating the fermentation of at least one of the carbohydrates present in the medium. The lack of black colonies indicates no H2S production. The lack of orange haze around the colonies indicates there is no precipitation of the bile salts by this organism. (Jan Hudzicki, University of Kansas Medical Center, Kansas City, KS)

Source: November 2010
Download as Powerpoint
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