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Urease Test Protocol

  • Author: Benita Brink1
  • VIEW AFFILIATIONS HIDE AFFILIATIONS
    Affiliations: 1: Adams State College, Alamosa, CO, 81102
  • Citation: Benita Brink. 2010. Urease test protocol.
  • Publication Date : November 2010
  • Category: Protocol
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Figures

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Figure 1

Urease test using urea agar slants. A rapidly positive urease test by Proteus mirabilis (b) is indicated by a color change to bright pink (fuchsia) throughout the urea agar slant as compared to the uninoculated control (a). A delayed positive reaction by Klebsiella pneumoniae is indicated by a color change only along the slant (c). A negative reaction by Escherichia coli is indicated by the yellow coloration of the media (d). All samples were incubated at 37oC for 16 hours. (Benita A. Brink, Adams State College, Alamosa, CO)

Source: November 2010
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Figure 2

Urease test using urea broth. A positive urease test by Proteus vulgaris (left) is indicated by a color change to bright pink (fuchsia). A negative reaction by Escherichia coli (right) is indicated by the yellow coloration of the media. All samples were incubated at 37oC for 16 hours. (Anne Hanson, University of Maine, Orono, ME)

Source: November 2010
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